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Overview

Uniprot IDQ13769
Protein NameTHO complex subunit 5
Gene NameTHOC5
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
223 LKEIEVKKEYLSSLQ
24 DGAPAEGKRNRSDTE
78 DLKSRGGKDVAIEIE

Function

Component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA (PubMed:15833825, PubMed:15998806, PubMed:17190602). Plays a key structural role in the oligomerization of the THO-DDX39B complex (PubMed:33191911). TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway (PubMed:15833825, PubMed:15998806, PubMed:17190602). THOC5 in conjunction with ALYREF/THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in transcription elongation and genome stability (PubMed:18974867). Involved in alternative polyadenylation site choice by recruiting CPSF6 to 5' region of target genes; probably mediates association of the TREX and CFIm complexes (PubMed:23685434)

Protein Sequence

10 MSSESSKKRK 20 PKVIRSDGAP 30 AEGKRNRSDT 40 EQEGKYYSEE 50 AEVDLRDPGR 60 DYELYKYTCQ 70 ELQRLMAEIQ 80 DLKSRGGKDV 90 AIEIEERRIQ 100 SCVHFMTLKK 110 LNRLAHIRLK 120 KGRDQTHEAK 130 QKVDAYHLQL 140 QNLLYEVMHL 150 QKEITKCLEF 160 KSKHEEIDLV 170 SLEEFYKEAP 180 PDISKAEVTM 190 GDPHQQTLAR 200 LDWELEQRKR 210 LAEKYRECLS 220 NKEKILKEIE 230 VKKEYLSSLQ 240 PRLNSIMQAS 250 LPVQEYLFMP 260 FDQAHKQYET 270 ARHLPPPLYV 280 LFVQATAYGQ 290 ACDKTLSVAI 300 EGSVDEAKAL 310 FKPPEDSQDD 320 ESDSDAEEEQ 330 TTKRRRPTLG 340 VQLDDKRKEM 350 LKRHPLSVML 360 DLKCKDDSVL 370 HLTFYYLMNL 380 NIMTVKAKVT 390 TAMELITPIS 400 AGDLLSPDSV 410 LSCLYPGDHG 420 KKTPNPANQY 430 QFDKVGILTL 440 SDYVLELGHP 450 YLWVQKLGGL 460 HFPKEQPQQT 470 VIADHSLSAS 480 HMETTMKLLK 490 TRVQSRLALH 500 KQFASLEHGI 510 VPVTSDCQYL 520 FPAKVVSRLV 530 KWVTVAHEDY 540 MELHFTKDIV 550 DAGLAGDTNL 560 YYMALIERGT 570 AKLQAAVVLN 580 PGYSSIPPVF 590 QLCLNWKGEK 600 TNSNDDNIRA 610 MEGEVNVCYK 620 ELCGPWPSHQ 630 LLTNQLQRLC 640 VLLDVYLETE 650 SHDDSVEGPK 660 EFPQEKMCLR 670 LFRGPSRMKP 680 FKYNHPQGFF SHR

Gene Ontology

Classification GO ID Description
Cellular Component GO:0000445 THO complex part of transcription export complex
Cellular Component GO:0000346 transcription export complex
Molecular Function GO:0003729 mRNA binding
Biological Process GO:0030224 monocyte differentiation
Biological Process GO:0006406 mRNA export from nucleus
Biological Process GO:0006397 mRNA processing
Biological Process GO:0060215 primitive hemopoiesis
Biological Process GO:0008380 RNA splicing
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0000347 THO complex

Reference

[1] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[2] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.