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Overview

Uniprot IDQ13951
Protein NameCore-binding factor subunit beta
Gene NameCBFB
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
177 QLLAVTGKKTTRP**
178 LLAVTGKKTTRP***

Function

Forms the heterodimeric complex core-binding factor (CBF) with RUNX family proteins (RUNX1, RUNX2, and RUNX3). RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation

Protein Sequence

10 MPRVVPDQRS 20 KFENEEFFRK 30 LSRECEIKYT 40 GFRDRPHEER 50 QARFQNACRD 60 GRSEIAFVAT 70 GTNLSLQFFP 80 ASWQGEQRQT 90 PSREYVDLER 100 EAGKVYLKAP 110 MILNGVCVIW 120 KGWIDLQRLD 130 GMGCLEFDEE 140 RAQQEDALAQ 150 QAFEEARRRT 160 REFEDRDRSH 170 REEMEVRVSQ 180 LLAVTGKKTT RP

Gene Ontology

Classification GO ID Description
Cellular Component GO:0016513 core-binding factor complex
Cellular Component GO:0016020 membrane
Cellular Component GO:0005654 nucleoplasm
Molecular Function GO:0043565 sequence-specific DNA binding
Molecular Function GO:0003713 transcription coactivator activity
Biological Process GO:0043371 negative regulation of CD4-positive, alpha-beta T cell differentiation
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:0043378 positive regulation of CD8-positive, alpha-beta T cell differentiation
Biological Process GO:0000209 protein polyubiquitination
Biological Process GO:0006357 regulation of transcription by RNA polymerase II
Biological Process GO:0006366 transcription by RNA polymerase II

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[3] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[4] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.