Search Results

Overview

Uniprot IDQ14008
Protein NameCytoskeleton-associated protein 5
Gene NameCKAP5
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1126 EPKPDPKKAKAPGLS
1135 KAPGLSSKAKSAQGK
1843 IFKKIGSKENTKEGL
1981 STDMLHSKLSQLRES
544 APKPGPLKKAPAAKA
550 LKKAPAAKAGGPPKK
661 NFQVMQMKLHIVALI

Function

Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Acts as a processive microtubule polymerase. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles. In spindle formation protects kinetochore microtubules from depolymerization by KIF2C and has an essential role in centrosomal microtubule assembly independently of KIF2C activity. Contributes to centrosome integrity. Acts as a component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed:23532825). Enhances the strength of NDC80 complex-mediated kinetochore-tip microtubule attachments (PubMed:27156448)

Protein Sequence

10 MGDDSEWLKL 20 PVDQKCEHKL 30 WKARLSGYEE 40 ALKIFQKIKD 50 EKSPEWSKFL 60 GLIKKFVTDS 70 NAVVQLKGLE 80 AALVYVENAH 90 VAGKTTGEVV 100 SGVVSKVFNQ 110 PKAKAKELGI 120 EICLMYIEIE 130 KGEAVQEELL 140 KGLDNKNPKI 150 IVACIETLRK 160 ALSEFGSKII 170 LLKPIIKVLP 180 KLFESREKAV 190 RDEAKLIAVE 200 IYRWIRDALR 210 PPLQNINSVQ 220 LKELEEEWVK 230 LPTSAPRPTR 240 FLRSQQELEA 250 KLEQQQSAGG 260 DAEGGGDDGD 270 EVPQIDAYEL 280 LEAVEILSKL 290 PKDFYDKIEA 300 KKWQERKEAL 310 ESVEVLIKNP 320 KLEAGDYADL 330 VKALKKVVGK 340 DTNVMLVALA 350 AKCLTGLAVG 360 LRKKFGQYAG 370 HVVPTILEKF 380 KEKKPQVVQA 390 LQEAIDAIFL 400 TTTLQNISED 410 VLAVMDNKNP 420 TIKQQTSLFI 430 ARSFRHCTAS 440 TLPKSLLKPF 450 CAALLKHIND 460 SAPEVRDAAF 470 EALGTALKVV 480 GEKAVNPFLA 490 DVDKLKLDKI 500 KECSEKVELI 510 HGKKAGLAAD 520 KKEFKPLPGR 530 TAASGAAGDK 540 DTKDISAPKP 550 GPLKKAPAAK 560 AGGPPKKGKP 570 AAPGGAGNTG 580 TKNKKGLETK 590 EIVEPELSIE 600 VCEEKASAVL 610 PPTCIQLLDS 620 SNWKERLACM 630 EEFQKAVELM 640 DRTEMPCQAL 650 VRMLAKKPGW 660 KETNFQVMQM 670 KLHIVALIAQ 680 KGNFSKTSAQ 690 VVLDGLVDKI 700 GDVKCGNNAK 710 EAMTAIAEAC 720 MLPWTAEQVV 730 SMAFSQKNPK 740 NQSETLNWLS 750 NAIKEFGFSG 760 LNVKAFISNV 770 KTALAATNPA 780 VRTAAITLLG 790 VMYLYVGPSL 800 RMFFEDEKPA 810 LLSQIDAEFE 820 KMQGQSPPAP 830 TRGISKHSTS 840 GTDEGEDGDE 850 PDDGSNDVVD 860 LLPRTEISDK 870 ITSELVSKIG 880 DKNWKIRKEG 890 LDEVAGIIND 900 AKFIQPNIGE 910 LPTALKGRLN 920 DSNKILVQQT 930 LNILQQLAVA 940 MGPNIKQHVK 950 NLGIPIITVL 960 GDSKNNVRAA 970 ALATVNAWAE 980 QTGMKEWLEG 990 EDLSEELKKE 1000 NPFLRQELLG 1010 WLAEKLPTLR 1020 STPTDLILCV 1030 PHLYSCLEDR 1040 NGDVRKKAQD 1050 ALPFFMMHLG 1060 YEKMAKATGK 1070 LKPTSKDQVL 1080 AMLEKAKVNM 1090 PAKPAPPTKA 1100 TSKPMGGSAP 1110 AKFQPASAPA 1120 EDCISSSTEP 1130 KPDPKKAKAP 1140 GLSSKAKSAQ 1150 GKKMPSKTSL 1160 KEDEDKSGPI 1170 FIVVPNGKEQ 1180 RMKDEKGLKV 1190 LKWNFTTPRD 1200 EYIEQLKTQM 1210 SSCVAKWLQD 1220 EMFHSDFQHH 1230 NKALAVMVDH 1240 LESEKEGVIG 1250 CLDLILKWLT 1260 LRFFDTNTSV 1270 LMKALEYLKL 1280 LFTLLSEEEY 1290 HLTENEASSF 1300 IPYLVVKVGE 1310 PKDVIRKDVR 1320 AILNRMCLVY 1330 PASKMFPFIM 1340 EGTKSKNSKQ 1350 RAECLEELGC 1360 LVESYGMNVC 1370 QPTPGKALKE 1380 IAVHIGDRDN 1390 AVRNAALNTI 1400 VTVYNVHGDQ 1410 VFKLIGNLSE 1420 KDMSMLEERI 1430 KRSAKRPSAA 1440 PIKQVEEKPQ 1450 RAQNISSNAN 1460 MLRKGPAEDM 1470 SSKLNQARSM 1480 SGHPEAAQMV 1490 RREFQLDLDE 1500 IENDNGTVRC 1510 EMPELVQHKL 1520 DDIFEPVLIP 1530 EPKIRAVSPH 1540 FDDMHSNTAS 1550 TINFIISQVA 1560 SGDINTSIQA 1570 LTQIDEVLRQ 1580 EDKAEAMSGH 1590 IDQFLIATFM 1600 QLRLIYNTHM 1610 ADEKLEKDEI 1620 IKLYSCIIGN 1630 MISLFQIESL 1640 AREASTGVLK 1650 DLMHGLITLM 1660 LDSRIEDLEE 1670 GQQVIRSVNL 1680 LVVKVLEKSD 1690 QTNILSALLV 1700 LLQDSLLATA 1710 SSPKFSELVM 1720 KCLWRMVRLL 1730 PDTINSINLD 1740 RILLDIHIFM 1750 KVFPKEKLKQ 1760 CKSEFPIRTL 1770 KTLLHTLCKL 1780 KGPKILDHLT 1790 MIDNKNESEL 1800 EAHLCRMMKH 1810 SMDQTGSKSD 1820 KETEKGASRI 1830 DEKSSKAKVN 1840 DFLAEIFKKI 1850 GSKENTKEGL 1860 AELYEYKKKY 1870 SDADIEPFLK 1880 NSSQFFQSYV 1890 ERGLRVIEME 1900 REGKGRISTS 1910 TGISPQMEVT 1920 CVPTPTSTVS 1930 SIGNTNGEEV 1940 GPSVYLERLK 1950 ILRQRCGLDN 1960 TKQDDRPPLT 1970 SLLSKPAVPT 1980 VASSTDMLHS 1990 KLSQLRESRE 2000 QHQHSDLDSN 2010 QTHSSGTVTS 2020 SSSTANIDDL 2030 KKRLERIKSS RK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005813 centrosome
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0000776 kinetochore
Cellular Component GO:0016020 membrane
Cellular Component GO:0032991 protein-containing complex
Cellular Component GO:0000922 spindle pole
Molecular Function GO:0045296 cadherin binding
Molecular Function GO:0008017 microtubule binding
Molecular Function GO:0061863 microtubule plus end polymerase activity
Molecular Function GO:0051010 microtubule plus-end binding
Molecular Function GO:0043021 ribonucleoprotein complex binding
Biological Process GO:0051301 cell division
Biological Process GO:0007098 centrosome cycle
Biological Process GO:0051298 centrosome duplication
Biological Process GO:0030951 establishment or maintenance of microtubule cytoskeleton polarity
Biological Process GO:0007019 microtubule depolymerization
Biological Process GO:0046785 microtubule polymerization
Biological Process GO:0007052 mitotic spindle organization
Biological Process GO:0090063 positive regulation of microtubule nucleation
Biological Process GO:0050658 RNA transport
Biological Process GO:0007051 spindle organization

Reference

[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[2] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[3] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.