Search Results

Overview

Uniprot IDQ14152
Protein NameEukaryotic translation initiation factor 3 subunit A
Gene NameEIF3A
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1174 GPWRPLVKPGGWREK
1317 WRRADDRKDDRVEER
1369 RESLRRTKNETDEDG
308 HLSREMRKNLTQDEM
603 QREAELQKVRKAEEE
632 LQEHEQIKKKTVRER
633 QEHEQIKKKTVRERL
68 LRKSHLAKEGLYQYK
850 EYQERVKKLEEVERK
901 DSEGTWRKGPEADSE

Function

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:11169732, PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773, PubMed:27462815)

Protein Sequence

10 MPAYFQRPEN 20 ALKRANEFLE 30 VGKKQPALDV 40 LYDVMKSKKH 50 RTWQKIHEPI 60 MLKYLELCVD 70 LRKSHLAKEG 80 LYQYKNICQQ 90 VNIKSLEDVV 100 RAYLKMAEEK 110 TEAAKEESQQ 120 MVLDIEDLDN 130 IQTPESVLLS 140 AVSGEDTQDR 150 TDRLLLTPWV 160 KFLWESYRQC 170 LDLLRNNSRV 180 ERLYHDIAQQ 190 AFKFCLQYTR 200 KAEFRKLCDN 210 LRMHLSQIQR 220 HHNQSTAINL 230 NNPESQSMHL 240 ETRLVQLDSA 250 ISMELWQEAF 260 KAVEDIHGLF 270 SLSKKPPKPQ 280 LMANYYNKVS 290 TVFWKSGNAL 300 FHASTLHRLY 310 HLSREMRKNL 320 TQDEMQRMST 330 RVLLATLSIP 340 ITPERTDIAR 350 LLDMDGIIVE 360 KQRRLATLLG 370 LQAPPTRIGL 380 INDMVRFNVL 390 QYVVPEVKDL 400 YNWLEVEFNP 410 LKLCERVTKV 420 LNWVREQPEK 430 EPELQQYVPQ 440 LQNNTILRLL 450 QQVSQIYQSI 460 EFSRLTSLVP 470 FVDAFQLERA 480 IVDAARHCDL 490 QVRIDHTSRT 500 LSFGSDLNYA 510 TREDAPIGPH 520 LQSMPSEQIR 530 NQLTAMSSVL 540 AKALEVIKPA 550 HILQEKEEQH 560 QLAVTAYLKN 570 SRKEHQRILA 580 RRQTIEERKE 590 RLESLNIQRE 600 KEELEQREAE 610 LQKVRKAEEE 620 RLRQEAKERE 630 KERILQEHEQ 640 IKKKTVRERL 650 EQIKKTELGA 660 KAFKDIDIED 670 LEELDPDFIM 680 AKQVEQLEKE 690 KKELQERLKN 700 QEKKIDYFER 710 AKRLEEIPLI 720 KSAYEEQRIK 730 DMDLWEQQEE 740 ERITTMQLER 750 EKALEHKNRM 760 SRMLEDRDLF 770 VMRLKAARQS 780 VYEEKLKQFE 790 ERLAEERHNR 800 LEERKRQRKE 810 ERRITYYREK 820 EEEEQRRAEE 830 QMLKEREERE 840 RAERAKREEE 850 LREYQERVKK 860 LEEVERKKRQ 870 RELEIEERER 880 RREEERRLGD 890 SSLSRKDSRW 900 GDRDSEGTWR 910 KGPEADSEWR 920 RGPPEKEWRR 930 GEGRDEDRSH 940 RRDEERPRRL 950 GDDEDREPSL 960 RPDDDRVPRR 970 GMDDDRGPRR 980 GPEEDRFSRR 990 GADDDRPSWR 1000 NTDDDRPPRR 1010 IADEDRGNWR 1020 HADDDRPPRR 1030 GLDEDRGSWR 1040 TADEDRGPRR 1050 GMDDDRGPRR 1060 GGADDERSSW 1070 RNADDDRGPR 1080 RGLDDDRGPR 1090 RGMDDDRGPR 1100 RGMDDDRGPR 1110 RGMDDDRGPR 1120 RGLDDDRGPW 1130 RNADDDRIPR 1140 RGAEDDRGPW 1150 RNMDDDRLSR 1160 RADDDRFPRR 1170 GDDSRPGPWR 1180 PLVKPGGWRE 1190 KEKAREESWG 1200 PPRESRPSEE 1210 REWDREKERD 1220 RDNQDREEND 1230 KDPERERDRE 1240 RDVDREDRFR 1250 RPRDEGGWRR 1260 GPAEESSSWR 1270 DSSRRDDRDR 1280 DDRRRERDDR 1290 RDLRERRDLR 1300 DDRDRRGPPL 1310 RSEREEVSSW 1320 RRADDRKDDR 1330 VEERDPPRRV 1340 PPPALSRDRE 1350 RDRDREREGE 1360 KEKASWRAEK 1370 DRESLRRTKN 1380 ETDEDGWTTV RR

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0016282 eukaryotic 43S preinitiation complex
Cellular Component GO:0033290 eukaryotic 48S preinitiation complex
Cellular Component GO:0005852 eukaryotic translation initiation factor 3 complex
Cellular Component GO:0071540 eukaryotic translation initiation factor 3 complex, eIF3e
Cellular Component GO:0071541 eukaryotic translation initiation factor 3 complex, eIF3m
Cellular Component GO:0016020 membrane
Cellular Component GO:0005874 microtubule
Cellular Component GO:0043614 multi-eIF complex
Cellular Component GO:0014069 postsynaptic density
Molecular Function GO:0003729 mRNA binding
Molecular Function GO:0030971 receptor tyrosine kinase binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0005198 structural molecule activity
Molecular Function GO:0003743 translation initiation factor activity
Biological Process GO:0001732 formation of cytoplasmic translation initiation complex
Biological Process GO:0075522 IRES-dependent viral translational initiation
Biological Process GO:0070373 negative regulation of ERK1 and ERK2 cascade
Biological Process GO:0002188 translation reinitiation
Biological Process GO:0006413 translational initiation
Biological Process GO:0075525 viral translational termination-reinitiation

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[3] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[4] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.