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Overview

Uniprot ID	Q14181
Protein Name	DNA polymerase alpha subunit B
Gene Name	POLA2
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
134	TPETPLTKRSVSTRS
83	KARHSTCKDSGHAGA

Function

Accessory subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis (PubMed:9705292). During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1 (By similarity). The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands (By similarity). These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively (By similarity)

Protein Sequence

10 MSASAQQLAE 20 ELQIFGLDCE 30 EALIEKLVEL 40 CVQYGQNEEG 50 MVGELIAFCT 60 STHKVGLTSE 70 ILNSFEHEFL 80 SKRLSKARHS 90 TCKDSGHAGA 100 RDIVSIQELI 110 EVEEEEEILL 120 NSYTTPSKGS 130 QKRAISTPET 140 PLTKRSVSTR 150 SPHQLLSPSS 160 FSPSATPSQK 170 YNSRSNRGEV 180 VTSFGLAQGV 190 SWSGRGGAGN 200 ISLKVLGCPE 210 ALTGSYKSMF 220 QKLPDIREVL 230 TCKIEELGSE 240 LKEHYKIEAF 250 TPLLAPAQEP 260 VTLLGQIGCD 270 SNGKLNNKSV 280 ILEGDREHSS 290 GAQIPVDLSE 300 LKEYSLFPGQ 310 VVIMEGINTT 320 GRKLVATKLY 330 EGVPLPFYQP 340 TEEDADFEQS 350 MVLVACGPYT 360 TSDSITYDPL 370 LDLIAVINHD 380 RPDVCILFGP 390 FLDAKHEQVE 400 NCLLTSPFED 410 IFKQCLRTII 420 EGTRSSGSHL 430 VFVPSLRDVH 440 HEPVYPQPPF 450 SYSDLSREDK 460 KQVQFVSEPC 470 SLSINGVIFG 480 LTSTDLLFHL 490 GAEEISSSSG 500 TSDRFSRILK 510 HILTQRSYYP 520 LYPPQEDMAI 530 DYESFYVYAQ 540 LPVTPDVLII 550 PSELRYFVKD 560 VLGCVCVNPG 570 RLTKGQVGGT 580 FARLYLRRPA 590 ADGAERQSPC IAVQVVRI

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005658	alpha DNA polymerase:primase complex
Cellular Component	GO:0005654	nucleoplasm
Molecular Function	GO:0003677	DNA binding
Biological Process	GO:0006260	DNA replication
Biological Process	GO:0006270	DNA replication initiation
Biological Process	GO:0006269	DNA replication, synthesis of primer

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[3] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.