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Overview

Uniprot ID	Q14203
Protein Name	Dynactin subunit 1
Gene Name	DCTN1
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
68	ILDEAKGKNDGTVQG
962	LKKSLKIKGEELSEA

Function

Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity). Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. Essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Can also act as a brake to slow the dynein motor during motility along the microtubule (PubMed:25185702). Can regulate microtubule stability by promoting microtubule formation, nucleation and polymerization and by inhibiting microtubule catastrophe in neurons. Inhibits microtubule catastrophe by binding both to microtubules and to tubulin, leading to enhanced microtubule stability along the axon (PubMed:23874158). Plays a role in metaphase spindle orientation (PubMed:22327364). Plays a role in centriole cohesion and subdistal appendage organization and function. Its recruitment to the centriole in a KIF3A-dependent manner is essential for the maintenance of centriole cohesion and the formation of subdistal appendage. Also required for microtubule anchoring at the mother centriole (PubMed:23386061). Plays a role in primary cilia formation (PubMed:25774020)

Protein Sequence

10 MAQSKRHVYS 20 RTPSGSRMSA 30 EASARPLRVG 40 SRVEVIGKGH 50 RGTVAYVGAT 60 LFATGKWVGV 70 ILDEAKGKND 80 GTVQGRKYFT 90 CDEGHGIFVR 100 QSQIQVFEDG 110 ADTTSPETPD 120 SSASKVLKRE 130 GTDTTAKTSK 140 LRGLKPKKAP 150 TARKTTTRRP 160 KPTRPASTGV 170 AGASSSLGPS 180 GSASAGELSS 190 SEPSTPAQTP 200 LAAPIIPTPV 210 LTSPGAVPPL 220 PSPSKEEEGL 230 RAQVRDLEEK 240 LETLRLKRAE 250 DKAKLKELEK 260 HKIQLEQVQE 270 WKSKMQEQQA 280 DLQRRLKEAR 290 KEAKEALEAK 300 ERYMEEMADT 310 ADAIEMATLD 320 KEMAEERAES 330 LQQEVEALKE 340 RVDELTTDLE 350 ILKAEIEEKG 360 SDGAASSYQL 370 KQLEEQNARL 380 KDALVRMRDL 390 SSSEKQEHVK 400 LQKLMEKKNQ 410 ELEVVRQQRE 420 RLQEELSQAE 430 STIDELKEQV 440 DAALGAEEMV 450 EMLTDRNLNL 460 EEKVRELRET 470 VGDLEAMNEM 480 NDELQENARE 490 TELELREQLD 500 MAGARVREAQ 510 KRVEAAQETV 520 ADYQQTIKKY 530 RQLTAHLQDV 540 NRELTNQQEA 550 SVERQQQPPP 560 ETFDFKIKFA 570 ETKAHAKAIE 580 MELRQMEVAQ 590 ANRHMSLLTA 600 FMPDSFLRPG 610 GDHDCVLVLL 620 LMPRLICKAE 630 LIRKQAQEKF 640 ELSENCSERP 650 GLRGAAGEQL 660 SFAAGLVYSL 670 SLLQATLHRY 680 EHALSQCSVD 690 VYKKVGSLYP 700 EMSAHERSLD 710 FLIELLHKDQ 720 LDETVNVEPL 730 TKAIKYYQHL 740 YSIHLAEQPE 750 DCTMQLADHI 760 KFTQSALDCM 770 SVEVGRLRAF 780 LQGGQEATDI 790 ALLLRDLETS 800 CSDIRQFCKK 810 IRRRMPGTDA 820 PGIPAALAFG 830 PQVSDTLLDC 840 RKHLTWVVAV 850 LQEVAAAAAQ 860 LIAPLAENEG 870 LLVAALEELA 880 FKASEQIYGT 890 PSSSPYECLR 900 QSCNILISTM 910 NKLATAMQEG 920 EYDAERPPSK 930 PPPVELRAAA 940 LRAEITDAEG 950 LGLKLEDRET 960 VIKELKKSLK 970 IKGEELSEAN 980 VRLSLLEKKL 990 DSAAKDADER 1000 IEKVQTRLEE 1010 TQALLRKKEK 1020 EFEETMDALQ 1030 ADIDQLEAEK 1040 AELKQRLNSQ 1050 SKRTIEGLRG 1060 PPPSGIATLV 1070 SGIAGEEQQR 1080 GAIPGQAPGS 1090 VPGPGLVKDS 1100 PLLLQQISAM 1110 RLHISQLQHE 1120 NSILKGAQMK 1130 ASLASLPPLH 1140 VAKLSHEGPG 1150 SELPAGALYR 1160 KTSQLLETLN 1170 QLSTHTHVVD 1180 ITRTSPAAKS 1190 PSAQLMEQVA 1200 QLKSLSDTVE 1210 KLKDEVLKET 1220 VSQRPGATVP 1230 TDFATFPSSA 1240 FLRAKEEQQD 1250 DTVYMGKVTF 1260 SCAAGFGQRH 1270 RLVLTQEQLH QLHSRLIS

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0030424	axon
Cellular Component	GO:0005938	cell cortex
Cellular Component	GO:0099738	cell cortex region
Cellular Component	GO:0031252	cell leading edge
Cellular Component	GO:0120103	centriolar subdistal appendage
Cellular Component	GO:0005814	centriole
Cellular Component	GO:0005813	centrosome
Cellular Component	GO:0036064	ciliary basal body
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0030286	dynein complex
Cellular Component	GO:0010008	endosome membrane
Cellular Component	GO:0045171	intercellular bridge
Cellular Component	GO:0000776	kinetochore
Cellular Component	GO:0016020	membrane
Cellular Component	GO:0005874	microtubule
Cellular Component	GO:0005875	microtubule associated complex
Cellular Component	GO:0015630	microtubule cytoskeleton
Cellular Component	GO:0035371	microtubule plus-end
Cellular Component	GO:0043005	neuron projection
Cellular Component	GO:0043025	neuronal cell body
Cellular Component	GO:0005635	nuclear envelope
Cellular Component	GO:0032991	protein-containing complex
Cellular Component	GO:0005819	spindle
Cellular Component	GO:0000922	spindle pole
Molecular Function	GO:0008017	microtubule binding
Molecular Function	GO:0019901	protein kinase binding
Molecular Function	GO:0048156	tau protein binding
Molecular Function	GO:0015631	tubulin binding
Biological Process	GO:0051301	cell division
Biological Process	GO:0010457	centriole-centriole cohesion
Biological Process	GO:0042632	cholesterol homeostasis
Biological Process	GO:0000132	establishment of mitotic spindle orientation
Biological Process	GO:0090120	lysosome to ER cholesterol transport
Biological Process	GO:0099558	maintenance of synapse structure
Biological Process	GO:0034454	microtubule anchoring at centrosome
Biological Process	GO:0000278	mitotic cell cycle
Biological Process	GO:0007077	mitotic nuclear membrane disassembly
Biological Process	GO:0061744	motor behavior
Biological Process	GO:0007399	nervous system development
Biological Process	GO:0007528	neuromuscular junction development
Biological Process	GO:0050905	neuromuscular process
Biological Process	GO:0070050	neuron cellular homeostasis
Biological Process	GO:1990535	neuron projection maintenance
Biological Process	GO:1905515	non-motile cilium assembly
Biological Process	GO:0007097	nuclear migration
Biological Process	GO:0090063	positive regulation of microtubule nucleation
Biological Process	GO:0031116	positive regulation of microtubule polymerization
Biological Process	GO:1904398	positive regulation of neuromuscular junction development
Biological Process	GO:0060236	regulation of mitotic spindle organization
Biological Process	GO:0042147	retrograde transport, endosome to Golgi
Biological Process	GO:0021517	ventral spinal cord development

Reference

[1] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[2] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.