Overview
| Uniprot ID | Q14671 |
|---|
| Protein Name | Pumilio homolog 1 |
|---|
| Gene Name | PUM1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 10 |
VACVLKRKAVLWQDS |
| 794 |
AAPGAEAKYRSASSA |
Function
Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (PubMed:18328718, PubMed:21397187, PubMed:21572425, PubMed:21653694). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation (PubMed:22955276). Also mediates deadenylation-independent repression by promoting accessibility of miRNAs (PubMed:18776931, PubMed:20818387, PubMed:20860814, PubMed:22345517). Following growth factor stimulation, phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA-binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle (PubMed:20818387). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation (PubMed:22345517, PubMed:29474920). Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm (PubMed:26724866). Involved in neuronal functions by regulating ATXN1 mRNA levels: acts by binding to the 3'-UTR of ATXN1 transcripts, leading to their down-regulation independently of the miRNA machinery (PubMed:25768905, PubMed:29474920). Plays a role in cytoplasmic sensing of viral infection (PubMed:25340845). In testis, acts as a post-transcriptional regulator of spermatogenesis by binding to the 3'-UTR of mRNAs coding for regulators of p53/TP53. Involved in embryonic stem cell renewal by facilitating the exit from the ground state: acts by targeting mRNAs coding for naive pluripotency transcription factors and accelerates their down-regulation at the onset of differentiation (By similarity). Binds specifically to miRNA MIR199A precursor, with PUM2, regulates miRNA MIR199A expression at a postranscriptional level (PubMed:28431233)
Protein Sequence
10
MSVACVLKRK
20
AVLWQDSFSP
30
HLKHHPQEPA
40
NPNMPVVLTS
50
GTGSQAQPQP
60
AANQALAAGT
70
HSSPVPGSIG
80
VAGRSQDDAM
90
VDYFFQRQHG
100
EQLGGGGSGG
110
GGYNNSKHRW
120
PTGDNIHAEH
130
QVRSMDELNH
140
DFQALALEGR
150
AMGEQLLPGK
160
KFWETDESSK
170
DGPKGIFLGD
180
QWRDSAWGTS
190
DHSVSQPIMV
200
QRRPGQSFHV
210
NSEVNSVLSP
220
RSESGGLGVS
230
MVEYVLSSSP
240
GDSCLRKGGF
250
GPRDADSDEN
260
DKGEKKNKGT
270
FDGDKLGDLK
280
EEGDVMDKTN
290
GLPVQNGIDA
300
DVKDFSRTPG
310
NCQNSANEVD
320
LLGPNQNGSE
330
GLAQLTSTNG
340
AKPVEDFSNM
350
ESQSVPLDPM
360
EHVGMEPLQF
370
DYSGTQVPVD
380
SAAATVGLFD
390
YNSQQQLFQR
400
PNALAVQQLT
410
AAQQQQYALA
420
AAHQPHIGLA
430
PAAFVPNPYI
440
ISAAPPGTDP
450
YTAGLAAAAT
460
LGPAVVPHQY
470
YGVTPWGVYP
480
ASLFQQQAAA
490
AAAATNSANQ
500
QTTPQAQQGQ
510
QQVLRGGASQ
520
RPLTPNQNQQ
530
GQQTDPLVAA
540
AAVNSALAFG
550
QGLAAGMPGY
560
PVLAPAAYYD
570
QTGALVVNAG
580
ARNGLGAPVR
590
LVAPAPVIIS
600
SSAAQAAVAA
610
AAASANGAAG
620
GLAGTTNGPF
630
RPLGTQQPQP
640
QPQQQPNNNL
650
ASSSFYGNNS
660
LNSNSQSSSL
670
FSQGSAQPAN
680
TSLGFGSSSS
690
LGATLGSALG
700
GFGTAVANSN
710
TGSGSRRDSL
720
TGSSDLYKRT
730
SSSLTPIGHS
740
FYNGLSFSSS
750
PGPVGMPLPS
760
QGPGHSQTPP
770
PSLSSHGSSS
780
SLNLGGLTNG
790
SGRYISAAPG
800
AEAKYRSASS
810
ASSLFSPSST
820
LFSSSRLRYG
830
MSDVMPSGRS
840
RLLEDFRNNR
850
YPNLQLREIA
860
GHIMEFSQDQ
870
HGSRFIQLKL
880
ERATPAERQL
890
VFNEILQAAY
900
QLMVDVFGNY
910
VIQKFFEFGS
920
LEQKLALAER
930
IRGHVLSLAL
940
QMYGCRVIQK
950
ALEFIPSDQQ
960
NEMVRELDGH
970
VLKCVKDQNG
980
NHVVQKCIEC
990
VQPQSLQFII
1000
DAFKGQVFAL
1010
STHPYGCRVI
1020
QRILEHCLPD
1030
QTLPILEELH
1040
QHTEQLVQDQ
1050
YGNYVIQHVL
1060
EHGRPEDKSK
1070
IVAEIRGNVL
1080
VLSQHKFASN
1090
VVEKCVTHAS
1100
RTERAVLIDE
1110
VCTMNDGPHS
1120
ALYTMMKDQY
1130
ANYVVQKMID
1140
VAEPGQRKIV
1150
MHKIRPHIAT
1160
LRKYTYGKHI
1170
LAKLEKYYMK
1180
NGVDLGPICG
PPNGII
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0030424 |
axon |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0010494 |
cytoplasmic stress granule |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0000932 |
P-body |
| Molecular Function |
GO:0035198 |
miRNA binding |
| Molecular Function |
GO:0003730 |
mRNA 3'-UTR binding |
| Molecular Function |
GO:0003723 |
RNA binding |
| Biological Process |
GO:0061158 |
3'-UTR-mediated mRNA destabilization |
| Biological Process |
GO:0008344 |
adult locomotory behavior |
| Biological Process |
GO:0035196 |
miRNA processing |
| Biological Process |
GO:0061157 |
mRNA destabilization |
| Biological Process |
GO:2000637 |
positive regulation of miRNA-mediated gene silencing |
| Biological Process |
GO:1900246 |
positive regulation of RIG-I signaling pathway |
| Biological Process |
GO:0016441 |
post-transcriptional gene silencing |
| Biological Process |
GO:0010608 |
post-transcriptional regulation of gene expression |
| Biological Process |
GO:0051726 |
regulation of cell cycle |
| Biological Process |
GO:0051983 |
regulation of chromosome segregation |
| Biological Process |
GO:0060964 |
regulation of miRNA-mediated gene silencing |
| Biological Process |
GO:0043488 |
regulation of mRNA stability |
| Biological Process |
GO:0006417 |
regulation of translation |
| Biological Process |
GO:0007283 |
spermatogenesis |
| Biological Process |
GO:0048863 |
stem cell differentiation |
Reference
[1] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.