Search Results

Overview

Uniprot ID	Q14997
Protein Name	Proteasome activator complex subunit 4
Gene Name	PSME4
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
1261	KTIPRTKKEWESSCF
1741	TKLPKKRKRDPGSVG

Function

Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks

Protein Sequence

10 MEPAERAGVG 20 EPPEPGGRPE 30 PGPRGFVPQK 40 EIVYNKLLPY 50 AERLDAESDL 60 QLAQIKCNLG 70 RAVQLQELWP 80 GGLFWTRKLS 90 TYIRLYGRKF 100 SKEDHVLFIK 110 LLYELVSIPK 120 LEISMMQGFA 130 RLLINLLKKK 140 ELLSRADLEL 150 PWRPLYDMVE 160 RILYSKTEHL 170 GLNWFPNSVE 180 NILKTLVKSC 190 RPYFPADATA 200 EMLEEWRPLM 210 CPFDVTMQKA 220 ITYFEIFLPT 230 SLPPELHHKG 240 FKLWFDELIG 250 LWVSVQNLPQ 260 WEGQLVNLFA 270 RLATDNIGYI 280 DWDPYVPKIF 290 TRILRSLNLP 300 VGSSQVLVPR 310 FLTNAYDIGH 320 AVIWITAMMG 330 GPSKLVQKHL 340 AGLFNSITSF 350 YHPSNNGRWL 360 NKLMKLLQRL 370 PNSVVRRLHR 380 ERYKKPSWLT 390 PVPDSHKLTD 400 QDVTDFVQCI 410 IQPVLLAMFS 420 KTGSLEAAQA 430 LQNLALMRPE 440 LVIPPVLERT 450 YPALETLTEP 460 HQLTATLSCV 470 IGVARSLVSG 480 GRWFPEGPTH 490 MLPLLMRALP 500 GVDPNDFSKC 510 MITFQFIATF 520 STLVPLVDCS 530 SVLQERNDLT 540 EVERELCSAT 550 AEFEDFVLQF 560 MDRCFGLIES 570 STLEQTREET 580 ETEKMTHLES 590 LVELGLSSTF 600 STILTQCSKE 610 IFMVALQKVF 620 NFSTSHIFET 630 RVAGRMVADM 640 CRAAVKCCPE 650 ESLKLFVPHC 660 CSVITQLTMN 670 DDVLNDEELD 680 KELLWNLQLL 690 SEITRVDGRK 700 LLLYREQLVK 710 ILQRTLHLTC 720 KQGYTLSCNL 730 LHHLLRSTTL 740 IYPTEYCSVP 750 GGFDKPPSEY 760 FPIKDWGKPG 770 DLWNLGIQWH 780 VPSSEEVSFA 790 FYLLDSFLQP 800 ELVKLQHCGD 810 GKLEMSRDDI 820 LQSLTIVHNC 830 LIGSGNLLPP 840 LKGEPVTNLV 850 PSMVSLEETK 860 LYTGLEYDLS 870 RENHREVIAT 880 VIRKLLNHIL 890 DNSEDDTKSL 900 FLIIKIIGDL 910 LQFQGSHKHE 920 FDSRWKSFNL 930 VKKSMENRLH 940 GKKQHIRALL 950 IDRVMLQHEL 960 RTLTVEGCEY 970 KKIHQDMIRD 980 LLRLSTSSYS 990 QVRNKAQQTF 1000 FAALGAYNFC 1010 CRDIIPLVLE 1020 FLRPDRQGVT 1030 QQQFKGALYC 1040 LLGNHSGVCL 1050 ANLHDWDCIV 1060 QTWPAIVSSG 1070 LSQAMSLEKP 1080 SIVRLFDDLA 1090 EKIHRQYETI 1100 GLDFTIPKSC 1110 VEIAELLQQS 1120 KNPSINQILL 1130 SPEKIKEGIK 1140 RQQEKNADAL 1150 RNYENLVDTL 1160 LDGVEQRNLP 1170 WKFEHIGIGL 1180 LSLLLRDDRV 1190 LPLRAIRFFV 1200 ENLNHDAIVV 1210 RKMAISAVAG 1220 ILKQLKRTHK 1230 KLTINPCEIS 1240 GCPKPTQIIA 1250 GDRPDNHWLH 1260 YDSKTIPRTK 1270 KEWESSCFVE 1280 KTHWGYYTWP 1290 KNMVVYAGVE 1300 EQPKLGRSRE 1310 DMTEAEQIIF 1320 DHFSDPKFVE 1330 QLITFLSLED 1340 RKGKDKFNPR 1350 RFCLFKGIFR 1360 NFDDAFLPVL 1370 KPHLEHLVAD 1380 SHESTQRCVA 1390 EIIAGLIRGS 1400 KHWTFEKVEK 1410 LWELLCPLLR 1420 TALSNITVET 1430 YNDWGACIAT 1440 SCESRDPRKL 1450 HWLFELLLES 1460 PLSGEGGSFV 1470 DACRLYVLQG 1480 GLAQQEWRVP 1490 ELLHRLLKYL 1500 EPKLTQVYKN 1510 VRERIGSVLT 1520 YIFMIDVSLP 1530 NTTPTISPHV 1540 PEFTARILEK 1550 LKPLMDVDEE 1560 IQNHVMEENG 1570 IGEEDERTQG 1580 IKLLKTILKW 1590 LMASAGRSFS 1600 TAVTEQLQLL 1610 PLFFKIAPVE 1620 NDNSYDELKR 1630 DAKLCLSLMS 1640 QGLLYPHQVP 1650 LVLQVLKQTA 1660 RSSSWHARYT 1670 VLTYLQTMVF 1680 YNLFIFLNNE 1690 DAVKDIRWLV 1700 ISLLEDEQLE 1710 VREMAATTLS 1720 GLLQCNFLTM 1730 DSPMQIHFEQ 1740 LCKTKLPKKR 1750 KRDPGSVGDT 1760 IPSAELVKRH 1770 AGVLGLGACV 1780 LSSPYDVPTW 1790 MPQLLMNLSA 1800 HLNDPQPIEM 1810 TVKKTLSNFR 1820 RTHHDNWQEH 1830 KQQFTDDQLL 1840 VLTDLLVSPC YYA

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0016607	nuclear speck
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:1990111	spermatoproteasome complex
Molecular Function	GO:0016504	peptidase activator activity
Molecular Function	GO:0070628	proteasome binding
Biological Process	GO:0006974	DNA damage response
Biological Process	GO:0006281	DNA repair
Biological Process	GO:0030317	flagellated sperm motility
Biological Process	GO:0010499	proteasomal ubiquitin-independent protein catabolic process
Biological Process	GO:0035092	sperm DNA condensation

Reference

[1] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.