Search Results

Overview

Uniprot ID	Q14999
Protein Name	Cullin-7
Gene Name	CUL7
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
1349	VGLGASGKEHKSEKE

Function

Core component of the 3M and Cul7-RING(FBXW8) complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:12481031, PubMed:12904573, PubMed:21572988, PubMed:21737058, PubMed:24793695, PubMed:35982156). Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity (PubMed:21572988, PubMed:21737058, PubMed:24793695). It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer (PubMed:24793695). The Cul7-RING(FBXW8) complex alone lacks ubiquitination activity and does not promote polyubiquitination and proteasomal degradation of p53/TP53 (PubMed:16547496, PubMed:17332328, PubMed:35982156). However it mediates recruitment of p53/TP53 for ubiquitination by neddylated CUL1-RBX1 (PubMed:35982156). Interaction with CUL9 is required to inhibit CUL9 activity and ubiquitination of BIRC5 (PubMed:24793696). The Cul7-RING(FBXW8) complex also mediates ubiquitination and consequent degradation of target proteins such as GORASP1, IRS1 and MAP4K1/HPK1 (PubMed:21572988, PubMed:24362026). Ubiquitination of GORASP1 regulates Golgi morphogenesis and dendrite patterning in brain (PubMed:21572988). Mediates ubiquitination and degradation of IRS1 in a mTOR-dependent manner: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2) (PubMed:18498745). The Cul7-RING(FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradation, thereby affecting cell proliferation and differentiation (PubMed:24362026). Acts as a regulator in trophoblast cell epithelial-mesenchymal transition and placental development (PubMed:20139075). While the Cul7-RING(FBXW8) and the 3M complexes are associated and involved in common processes, CUL7 and the Cul7-RING(FBXW8) complex may have additional functions. Probably plays a role in the degradation of proteins involved in endothelial proliferation and/or differentiation

Protein Sequence

10 MVGELRYREF 20 RVPLGPGLHA 30 YPDELIRQRV 40 GHDGHPEYQI 50 RWLILRRGDE 60 GDGGSGQVDC 70 KAEHILLWMS 80 KDEIYANCHK 90 MLGEDGQVIG 100 PSQESAGEVG 110 ALDKSVLEEM 120 ETDVKSLIQR 130 ALRQLEECVG 140 TIPPAPLLHT 150 VHVLSAYASI 160 EPLTGVFKDP 170 RVLDLLMHML 180 SSPDYQIRWS 190 AGRMIQALSS 200 HDAGTRTQIL 210 LSLSQQEAIE 220 KHLDFDSRCA 230 LLALFAQATL 240 SEHPMSFEGI 250 QLPQVPGRVL 260 FSLVKRYLHV 270 TSLLDQLNDS 280 AAEPGAQNTS 290 APEELSGERG 300 QLELEFSMAM 310 GTLISELVQA 320 MRWDQASDRP 330 RSSARSPGSI 340 FQPQLADVSP 350 GLPAAQAQPS 360 FRRSRRFRPR 370 SEFASGNTYA 380 LYVRDTLQPG 390 MRVRMLDDYE 400 EISAGDEGEF 410 RQSNNGVPPV 420 QVFWESTGRT 430 YWVHWHMLEI 440 LGFEEDIEDM 450 VEADEYQGAV 460 ASRVLGRALP 470 AWRWRPMTEL 480 YAVPYVLPED 490 EDTEECEHLT 500 LAEWWELLFF 510 IKKLDGPDHQ 520 EVLQILQENL 530 DGEILDDEIL 540 AELAVPIELA 550 QDLLLTLPQR 560 LNDSALRDLI 570 NCHVYKKYGP 580 EALAGNQAYP 590 SLLEAQEDVL 600 LLDAQAQAKD 610 SEDAAKVEAK 620 EPPSQSPNTP 630 LQRLVEGYGP 640 AGKILLDLEQ 650 ALSSEGTQEN 660 KVKPLLLQLQ 670 RQPQPFLALM 680 QSLDTPETNR 690 TLHLTVLRIL 700 KQLVDFPEAL 710 LLPWHEAVDA 720 CMACLRSPNT 730 DREVLQELIF 740 FLHRLTSVSR 750 DYAVVLNQLG 760 ARDAISKALE 770 KHLGKLELAQ 780 ELRDMVFKCE 790 KHAHLYRKLI 800 TNILGGCIQM 810 VLGQIEDHRR 820 THQPINIPFF 830 DVFLRYLCQG 840 SSVEVKEDKC 850 WEKVEVSSNP 860 HRASKLTDHN 870 PKTYWESNGS 880 AGSHYITLHM 890 RRGILIRQLT 900 LLVASEDSSY 910 MPARVVVCGG 920 DSTSSLHTEL 930 NSVNVMPSAS 940 RVILLENLTR 950 FWPIIQIRIK 960 RCQQGGIDTR 970 IRGLEILGPK 980 PTFWPVFREQ 990 LCRHTRLFYM 1000 VRAQAWSQDM 1010 AEDRRSLLHL 1020 SSRLNGALRQ 1030 EQNFADRFLP 1040 DDEAAQALGK 1050 TCWEALVSPV 1060 VQNITSPDED 1070 GISPLGWLLD 1080 QYLECQEAVF 1090 NPQSRGPAFF 1100 SRVRRLTHLL 1110 VHVEPCEAPP 1120 PVVATPRPKG 1130 RNRSHDWSSL 1140 ATRGLPSSIM 1150 RNLTRCWRAV 1160 VEKQVNNFLT 1170 SSWRDDDFVP 1180 RYCEHFNILQ 1190 NSSSELFGPR 1200 AAFLLALQNG 1210 CAGALLKLPF 1220 LKAAHVSEQF 1230 ARHIDQQIQG 1240 SRIGGAQEME 1250 RLAQLQQCLQ 1260 AVLIFSGLEI 1270 ATTFEHYYQH 1280 YMADRLLGVV 1290 SSWLEGAVLE 1300 QIGPCFPNRL 1310 PQQMLQSLST 1320 SKELQRQFHV 1330 YQLQQLDQEL 1340 LKLEDTEKKI 1350 QVGLGASGKE 1360 HKSEKEEEAG 1370 AAAVVDVAEG 1380 EEEEEENEDL 1390 YYEGAMPEVS 1400 VLVLSRHSWP 1410 VASICHTLNP 1420 RTCLPSYLRG 1430 TLNRYSNFYN 1440 KSQSHPALER 1450 GSQRRLQWTW 1460 LGWAELQFGN 1470 QTLHVSTVQM 1480 WLLLYLNDLK 1490 AVSVESLLAF 1500 SGLSADMLNQ 1510 AIGPLTSSRG 1520 PLDLHEQKDI 1530 PGGVLKIRDG 1540 SKEPRSRWDI 1550 VRLIPPQTYL 1560 QAEGEDGQNL 1570 EKRRNLLNCL 1580 IVRILKAHGD 1590 EGLHIDQLVC 1600 LVLEAWQKGP 1610 CPPRGLVSSL 1620 GKGSACSSTD 1630 VLSCILHLLG 1640 KGTLRRHDDR 1650 PQVLSYAVPV 1660 TVMEPHTESL 1670 NPGSSGPNPP 1680 LTFHTLQIRS 1690 RGVPYASCTA TQSFSTFR

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:1990393	3M complex
Cellular Component	GO:0005680	anaphase-promoting complex
Cellular Component	GO:0005813	centrosome
Cellular Component	GO:0031467	Cul7-RING ubiquitin ligase complex
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0005794	Golgi apparatus
Cellular Component	GO:0048471	perinuclear region of cytoplasm
Cellular Component	GO:0005886	plasma membrane
Molecular Function	GO:0160072	ubiquitin ligase complex scaffold activity
Biological Process	GO:0001837	epithelial to mesenchymal transition
Biological Process	GO:0007030	Golgi organization
Biological Process	GO:0000226	microtubule cytoskeleton organization
Biological Process	GO:0000281	mitotic cytokinesis
Biological Process	GO:0046627	negative regulation of insulin receptor signaling pathway
Biological Process	GO:0001890	placenta development
Biological Process	GO:0050775	positive regulation of dendrite morphogenesis
Biological Process	GO:0016567	protein ubiquitination
Biological Process	GO:0006508	proteolysis
Biological Process	GO:0007088	regulation of mitotic nuclear division
Biological Process	GO:0031146	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
Biological Process	GO:0006511	ubiquitin-dependent protein catabolic process
Biological Process	GO:0001570	vasculogenesis

Reference

[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.