Overview
| Uniprot ID | Q15042 |
|---|
| Protein Name | Rab3 GTPase-activating protein catalytic subunit |
|---|
| Gene Name | RAB3GAP1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 366 |
DITHALSKLTEPASV |
Function
Catalytic subunit of the Rab3 GTPase-activating (Rab3GAP) complex composed of RAB3GAP1 and RAB3GAP2, which accelerates the otherwise slow GTP hydrolysis catalyzed by Rab proteins (PubMed:9030515, PubMed:10859313, PubMed:39779760). Has GTPase-activating protein (GAP) activity towards various Rab3 subfamily members (RAB3A, RAB3B, RAB3C and RAB3D), RAB5A and RAB43 (PubMed:10859313, PubMed:9030515, PubMed:39779760). Additionally, it has guanine nucleotide exchange factor (GEF) activity towards RAB18, promoting GDP release from RAB18 and the conversion of inactive RAB18-GDP to the active form RAB18-GTP (PubMed:24891604, PubMed:39779760). Recruits and stabilizes RAB18 at the cis-Golgi membrane in fibroblasts where RAB18 is most likely activated (PubMed:26063829). Also involved in RAB18 recruitment at the endoplasmic reticulum (ER) membrane where it maintains proper ER structure (PubMed:24891604). Required for normal eye and brain development (PubMed:15696165, PubMed:23420520). May participate in neurodevelopmental processes such as cell proliferation, migration and differentiation before synapse formation, and is involved in regulating non-synaptic vesicular release of neurotransmitters (PubMed:9030515, PubMed:9852129)
Protein Sequence
10
MAADSEPESE
20
VFEITDFTTA
30
SEWERFISKV
40
EEVLNDWKLI
50
GNSLGKPLEK
60
GIFTSGTWEE
70
KSDEISFADF
80
KFSVTHHYLV
90
QESTDKEGKD
100
ELLEDVVPQS
110
MQDLLGMNND
120
FPPRAHCLVR
130
WYGLREFVVI
140
APAAHSDAVL
150
SESKCNLLLS
160
SVSIALGNTG
170
CQVPLFVQIH
180
HKWRRMYVGE
190
CQGPGVRTDF
200
EMVHLRKVPN
210
QYTHLSGLLD
220
IFKSKIGCPL
230
TPLPPVSIAI
240
RFTYVLQDWQ
250
QYFWPQQPPD
260
IDALVGGEVG
270
GLEFGKLPFG
280
ACEDPISELH
290
LATTWPHLTE
300
GIIVDNDVYS
310
DLDPIQAPHW
320
SVRVRKAENP
330
QCLLGDFVTE
340
FFKICRRKES
350
TDEILGRSAF
360
EEEGKETADI
370
THALSKLTEP
380
ASVPIHKLSV
390
SNMVHTAKKK
400
IRKHRGVEES
410
PLNNDVLNTI
420
LLFLFPDAVS
430
EKPLDGTTST
440
DNNNPPSESE
450
DYNLYNQFKS
460
APSDSLTYKL
470
ALCLCMINFY
480
HGGLKGVAHL
490
WQEFVLEMRF
500
RWENNFLIPG
510
LASGPPDLRC
520
CLLHQKLQML
530
NCCIERKKAR
540
DEGKKTSASD
550
VTNIYPGDAG
560
KAGDQLVPDN
570
LKETDKEKGE
580
VGKSWDSWSD
590
SEEEFFECLS
600
DTEELKGNGQ
610
ESGKKGGPKE
620
MANLRPEGRL
630
YQHGKLTLLH
640
NGEPLYIPVT
650
QEPAPMTEDL
660
LEEQSEVLAK
670
LGTSAEGAHL
680
RARMQSACLL
690
SDMESFKAAN
700
PGCSLEDFVR
710
WYSPRDYIEE
720
EVIDEKGNVV
730
LKGELSARMK
740
IPSNMWVEAW
750
ETAKPIPARR
760
QRRLFDDTRE
770
AEKVLHYLAI
780
QKPADLARHL
790
LPCVIHAAVL
800
KVKEEESLEN
810
ISSVKKIIKQ
820
IISHSSKVLH
830
FPNPEDKKLE
840
EIIHQITNVE
850
ALIARARSLK
860
AKFGTEKCEQ
870
EEEKEDLERF
880
VSCLLEQPEV
890
LVTGAGRGHA
900
GRIIHKLFVN
910
AQRAAAMTPP
920
EEELKRMGSP
930
EERRQNSVSD
940
FPPPAGREFI
950
LRTTVPRPAP
960
YSKALPQRMY
970
SVLTKEDFRL
980
AGAFSSDTSF
F
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005801 |
cis-Golgi network |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005789 |
endoplasmic reticulum membrane |
| Cellular Component |
GO:0071782 |
endoplasmic reticulum tubular network |
| Cellular Component |
GO:0070062 |
extracellular exosome |
| Cellular Component |
GO:0005794 |
Golgi apparatus |
| Cellular Component |
GO:0005811 |
lipid droplet |
| Cellular Component |
GO:0098794 |
postsynapse |
| Cellular Component |
GO:0032991 |
protein-containing complex |
| Molecular Function |
GO:0005096 |
GTPase activator activity |
| Molecular Function |
GO:0005085 |
guanyl-nucleotide exchange factor activity |
| Molecular Function |
GO:0031267 |
small GTPase binding |
| Biological Process |
GO:0007420 |
brain development |
| Biological Process |
GO:0043010 |
camera-type eye development |
| Biological Process |
GO:0097051 |
establishment of protein localization to endoplasmic reticulum membrane |
| Biological Process |
GO:0060079 |
excitatory postsynaptic potential |
| Biological Process |
GO:0060325 |
face morphogenesis |
| Biological Process |
GO:0021854 |
hypothalamus development |
| Biological Process |
GO:0034389 |
lipid droplet organization |
| Biological Process |
GO:2000786 |
positive regulation of autophagosome assembly |
| Biological Process |
GO:1903373 |
positive regulation of endoplasmic reticulum tubular network organization |
| Biological Process |
GO:0061646 |
positive regulation of glutamate neurotransmitter secretion in response to membrane depolarization |
| Biological Process |
GO:1903061 |
positive regulation of protein lipidation |
| Biological Process |
GO:0032482 |
Rab protein signal transduction |
| Biological Process |
GO:1903233 |
regulation of calcium ion-dependent exocytosis of neurotransmitter |
| Biological Process |
GO:0048172 |
regulation of short-term neuronal synaptic plasticity |
Reference
[1] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.