Search Results

Overview

Uniprot IDQ15059
Protein NameBromodomain-containing protein 3
Gene NameBRD3
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
153 ELLPPAPKGKGRKPA
155 LPPAPKGKGRKPAAG
245 KKKGVKRKADTTTPT
304 EVPQHAGKKGKLSEH
305 VPQHAGKKGKLSEHL
522 AEEEKKAKVAPPAKQ
528 AKVAPPAKQAQQKKA
643 CLQKKQRKPFSASGK
683 SGQLSSSKKPARKEK
684 GQLSSSKKPARKEKP

Function

Chromatin reader that recognizes and binds acetylated histones, thereby controlling gene expression and remodeling chromatin structures (PubMed:18406326, PubMed:22464331, PubMed:27105114, PubMed:32895492). Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation (PubMed:29567837, PubMed:32895492). In vitro, binds acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4 (PubMed:18406326). Involved in endoderm differentiation via its association with long non-coding RNA (lncRNA) DIGIT: BRD3 undergoes liquid-liquid phase separation upon binding to lncRNA DIGIT, promoting binding to histone H3 acetylated at 'Lys-18' (H3K18ac) to induce endoderm gene expression (PubMed:32895492). Also binds non-histones acetylated proteins, such as GATA1 and GATA2: regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity)

Protein Sequence

10 MSTATTVAPA 20 GIPATPGPVN 30 PPPPEVSNPS 40 KPGRKTNQLQ 50 YMQNVVVKTL 60 WKHQFAWPFY 70 QPVDAIKLNL 80 PDYHKIIKNP 90 MDMGTIKKRL 100 ENNYYWSASE 110 CMQDFNTMFT 120 NCYIYNKPTD 130 DIVLMAQALE 140 KIFLQKVAQM 150 PQEEVELLPP 160 APKGKGRKPA 170 AGAQSAGTQQ 180 VAAVSSVSPA 190 TPFQSVPPTV 200 SQTPVIAATP 210 VPTITANVTS 220 VPVPPAAAPP 230 PPATPIVPVV 240 PPTPPVVKKK 250 GVKRKADTTT 260 PTTSAITASR 270 SESPPPLSDP 280 KQAKVVARRE 290 SGGRPIKPPK 300 KDLEDGEVPQ 310 HAGKKGKLSE 320 HLRYCDSILR 330 EMLSKKHAAY 340 AWPFYKPVDA 350 EALELHDYHD 360 IIKHPMDLST 370 VKRKMDGREY 380 PDAQGFAADV 390 RLMFSNCYKY 400 NPPDHEVVAM 410 ARKLQDVFEM 420 RFAKMPDEPV 430 EAPALPAPAA 440 PMVSKGAESS 450 RSSEESSSDS 460 GSSDSEEERA 470 TRLAELQEQL 480 KAVHEQLAAL 490 SQAPVNKPKK 500 KKEKKEKEKK 510 KKDKEKEKEK 520 HKVKAEEEKK 530 AKVAPPAKQA 540 QQKKAPAKKA 550 NSTTTAGRQL 560 KKGGKQASAS 570 YDSEEEEEGL 580 PMSYDEKRQL 590 SLDINRLPGE 600 KLGRVVHIIQ 610 SREPSLRDSN 620 PDEIEIDFET 630 LKPTTLRELE 640 RYVKSCLQKK 650 QRKPFSASGK 660 KQAAKSKEEL 670 AQEKKKELEK 680 RLQDVSGQLS 690 SSKKPARKEK 700 PGSAPSGGPS 710 RLSSSSSSES 720 GSSSSSGSSS DSSDSE

Gene Ontology

Classification GO ID Description
Cellular Component GO:0000785 chromatin
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0003682 chromatin binding
Molecular Function GO:0042393 histone binding
Molecular Function GO:0140017 histone H3K18cr reader activity
Molecular Function GO:0140038 histone H3K27cr reader activity
Molecular Function GO:0140019 histone H3K9cr reader activity
Molecular Function GO:0062072 histone H3K9me2/3 reader activity
Molecular Function GO:0140566 histone reader activity
Molecular Function GO:0106222 lncRNA binding
Molecular Function GO:0140693 molecular condensate scaffold activity
Molecular Function GO:0004674 protein serine/threonine kinase activity
Biological Process GO:0006338 chromatin remodeling
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0035987 endodermal cell differentiation
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0071168 protein localization to chromatin
Biological Process GO:0006357 regulation of transcription by RNA polymerase II

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[5] Chao L, Xu Y, Yang Y, Ao X, Liang J. Identification of lactylation-related biomarkers for diagnosis, prognosis, and treatment responsiveness in triple-negative breast cancer.. World J Surg Oncol 24(1):77. 2026 Jan 22. PMID: 41566505.

[6] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.