Search Results

Overview

Uniprot IDQ15061
Protein NameWD repeat-containing protein 43
Gene NameWDR43
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
391 KVETAITKVRTPVMN
430 EQVESKRKSGGNEVS

Function

Ribosome biogenesis factor that coordinates hyperactive transcription and ribogenesis (PubMed:17699751). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Involved in nucleolar processing of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I (PubMed:17699751, PubMed:34516797). Essential for stem cell pluripotency and embryonic development. In the nucleoplasm, recruited by promoter-associated/nascent transcripts and transcription to active promoters where it facilitates releases of elongation factor P-TEFb and paused RNA polymerase II to allow transcription elongation and maintain high-level expression of its targets genes (By similarity)

Protein Sequence

10 MAAGGGGSCD 20 PLAPAGVPCA 30 FSPHSQAYFA 40 LASTDGHLRV 50 WETANNRLHQ 60 EYVPSAHLSG 70 TCTCLAWAPA 80 RLQAKESPQR 90 KKRKSEAVGM 100 SNQTDLLALG 110 TAVGSILLYS 120 TVKGELHSKL 130 ISGGHDNRVN 140 CIQWHQDSGC 150 LYSCSDDKHI 160 VEWNVQTCKV 170 KCKWKGDNSS 180 VSSLCISPDG 190 KMLLSAGRTI 200 KLWVLETKEV 210 YRHFTGHATP 220 VSSLMFTTIR 230 PPNESQPFDG 240 ITGLYFLSGA 250 VHDRLLNVWQ 260 VRSENKEKSA 270 VMSFTVTDEP 280 VYIDLTLSEN 290 KEEPVKLAVV 300 CRDGQVHLFE 310 HILNGYCKKP 320 LTSNCTIQIA 330 TPGKGKKSTP 340 KPIPILAAGF 350 CSDKMSLLLV 360 YGSWFQPTIE 370 RVALNSREPH 380 MCLVRDISNC 390 WAPKVETAIT 400 KVRTPVMNSE 410 AKVLVPGIPG 420 HHAAIKPAPP 430 QTEQVESKRK 440 SGGNEVSIEE 450 RLGAMDIDTH 460 KKGKEDLQTN 470 SFPVLLTQGL 480 ESNDFEMLNK 490 VLQTRNVNLI 500 KKTVLRMPLH 510 TIIPLLQELT 520 KRLQGHPNSA 530 VLMVQWLKCV 540 LTVHASYLST 550 LPDLVPQLGT 560 LYQLMESRVK 570 TFQKLSHLHG 580 KLILLITQVT 590 ASEKTKGATS 600 PGQKAKLVYE 610 EESSEEESDD 620 EIADKDSEDN 630 WDEDEEESES 640 EKDEDVEEED 650 EDAEGKDEEN 660 GEDRDTASEK 670 ELNGDSDLDP ENESEEE

Gene Ontology

Classification GO ID Description
Cellular Component GO:0000785 chromatin
Cellular Component GO:0001650 fibrillar center
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0032040 small-subunit processome
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0000993 RNA polymerase II complex binding
Molecular Function GO:0003711 transcription elongation factor activity
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0030490 maturation of SSU-rRNA
Biological Process GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
Biological Process GO:2000234 positive regulation of rRNA processing
Biological Process GO:0045943 positive regulation of transcription by RNA polymerase I
Biological Process GO:2000036 regulation of stem cell population maintenance
Biological Process GO:0034243 regulation of transcription elongation by RNA polymerase II
Biological Process GO:0042274 ribosomal small subunit biogenesis

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[5] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[6] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.