Search Results
Overview
| Uniprot ID | Q15233 |
|---|---|
| Protein Name | Non-POU domain-containing octamer-binding protein |
| Gene Name | NONO |
| Organism | Homo sapiens |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 107 | AGEVFIHKDKGFGFI |
| 109 | EVFIHKDKGFGFIRL |
| 11 | NKTFNLEKQNHTPRK |
| 198 | GIVEFSGKPAARKAL |
| 243 | LPEKLVIKNQQFHKE |
| 249 | IKNQQFHKEREQPPR |
| 272 | YEYAMRWKALIEMEK |
| 295 | NIKEAREKLEMEMEA |
| 336 | LHNQEVQKRKQLELR |
| 338 | NQEVQKRKQLELRQE |
| 371 | RRQQEGFKGTFPDAR |
| 467 | GAEFAPNKRRRY*** |
| 5 | ***MQSNKTFNLEKQ |
| 68 | NFRKPGEKTFTQRSR |
| 96 | EMRKLFEKYGKAGEV |
Function
DNA- and RNA binding protein, involved in several nuclear processes (PubMed:11525732, PubMed:12403470, PubMed:26571461). Binds the conventional octamer sequence in double-stranded DNA (PubMed:11525732, PubMed:12403470, PubMed:26571461). Also binds single-stranded DNA and RNA at a site independent of the duplex site (PubMed:11525732, PubMed:12403470, PubMed:26571461). Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ (PubMed:11525732, PubMed:12403470, PubMed:26571461). Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b (PubMed:12403470). Together with PSPC1, required for the formation of nuclear paraspeckles (PubMed:22416126). The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs (PubMed:11525732). The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 (PubMed:10858305). The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends (PubMed:15590677). In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex (PubMed:15590677). NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity (PubMed:11897684). NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription (By similarity). Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer (By similarity). Important for the functional organization of GABAergic synapses (By similarity). Plays a specific and important role in the regulation of synaptic RNAs and GPHN/gephyrin scaffold structure, through the regulation of GABRA2 transcript (By similarity). Plays a key role during neuronal differentiation by recruiting TET1 to genomic loci and thereby regulating 5-hydroxymethylcytosine levels (By similarity). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed:28712728, PubMed:30270045). Promotes activation of the cGAS-STING pathway in response to HIV-2 infection: acts by interacting with HIV-2 Capsid protein p24, thereby promoting detection of viral DNA by CGAS, leading to CGAS-mediated inmmune activation (PubMed:30270045). In contrast, the weak interaction with HIV-1 Capsid protein p24 does not allow activation of the cGAS-STING pathway (PubMed:30270045)
Protein Sequence
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Cellular Component | GO:0005694 | chromosome |
| Cellular Component | GO:0001650 | fibrillar center |
| Cellular Component | GO:0016020 | membrane |
| Cellular Component | GO:0016363 | nuclear matrix |
| Cellular Component | GO:0016607 | nuclear speck |
| Cellular Component | GO:0005730 | nucleolus |
| Cellular Component | GO:0005654 | nucleoplasm |
| Cellular Component | GO:0005634 | nucleus |
| Cellular Component | GO:0042382 | paraspeckles |
| Cellular Component | GO:0090575 | RNA polymerase II transcription regulator complex |
| Molecular Function | GO:0003682 | chromatin binding |
| Molecular Function | GO:0003677 | DNA binding |
| Molecular Function | GO:0042802 | identical protein binding |
| Molecular Function | GO:0106222 | lncRNA binding |
| Molecular Function | GO:0003723 | RNA binding |
| Biological Process | GO:0002218 | activation of innate immune response |
| Biological Process | GO:1904385 | cellular response to angiotensin |
| Biological Process | GO:0071456 | cellular response to hypoxia |
| Biological Process | GO:0007623 | circadian rhythm |
| Biological Process | GO:0006310 | DNA recombination |
| Biological Process | GO:0006281 | DNA repair |
| Biological Process | GO:0006351 | DNA-templated transcription |
| Biological Process | GO:0045087 | innate immune response |
| Biological Process | GO:0006397 | mRNA processing |
| Biological Process | GO:0045892 | negative regulation of DNA-templated transcription |
| Biological Process | GO:1903377 | negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway |
| Biological Process | GO:0042752 | regulation of circadian rhythm |
| Biological Process | GO:0006355 | regulation of DNA-templated transcription |
| Biological Process | GO:0008380 | RNA splicing |
Reference
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