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Overview

Uniprot IDQ15386
Protein NameUbiquitin-protein ligase E3C
Gene NameUBE3C
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
13 GDFKTRPKVSLGGAS
25 GASRKEEKASLLHRT

Function

E3 ubiquitin-protein ligase that specifically catalyzes 'Lys-29'- and 'Lys-48'-linked polyubiquitin chains (PubMed:11278995, PubMed:12692129, PubMed:16341092, PubMed:16601690, PubMed:24158444, PubMed:24811749, PubMed:25752573, PubMed:25752577, PubMed:32039437, PubMed:33637724, PubMed:34239127). Accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:32039437, PubMed:9575161). Associates with the proteasome and promotes elongation of ubiquitin chains on substrates bound to the 26S proteasome (PubMed:24158444, PubMed:28396413, PubMed:31375563). Also catalyzes 'Lys-29'- and 'Lys-48'-linked ubiquitination of 26S proteasome subunit ADRM1/RPN13 in response to proteotoxic stress, impairing the ability of the proteasome to bind and degrade ubiquitin-conjugated proteins (PubMed:24811749, PubMed:31375563). Acts as a negative regulator of autophagy by mediating 'Lys-29'- and 'Lys-48'-linked ubiquitination of PIK3C3/VPS34, promoting its degradation (PubMed:33637724). Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains; with some preference for 'Lys-48' linkages (PubMed:11278995, PubMed:16601690, PubMed:25752577). Acts as a negative regulator of type I interferon by mediating 'Lys-48'-linked ubiquitination of IRF3 and IRF7, leading to their degradation by the proteasome (PubMed:21167755). Catalyzes ubiquitination and degradation of CAND2 (PubMed:12692129)

Protein Sequence

10 MFSFEGDFKT 20 RPKVSLGGAS 30 RKEEKASLLH 40 RTQEERRKRE 50 EERRRLKNAI 60 IIQSFIRGYR 70 DRKQQYSIQR 80 SAFDRCATLS 90 QSGGAFPIAN 100 GPNLTLLVRQ 110 LLFFYKQNED 120 SKRLIWLYQN 130 LIKHSSLFVK 140 QLDGSERLTC 150 LFQIKRLMSL 160 CCRLLQNCND 170 DSLNVALPMR 180 MLEVFSSENT 190 YLPVLQDASY 200 VVSVIEQILH 210 YMIHNGYYRS 220 LYLLINSKLP 230 SSIEYSDLSR 240 VPIAKILLEN 250 VLKPLHFTYN 260 SCPEGARQQV 270 FTAFTEEFLA 280 APFTDQIFHF 290 IIPALADAQT 300 VFPYEPFLNA 310 LLLIESRCSR 320 KSGGAPWLFY 330 FVLTVGENYL 340 GALSEEGLLV 350 YLRVLQTFLS 360 QLPVSPASAS 370 CHDSASDSEE 380 ESEEADKPSS 390 PEDGRLSVSY 400 ITEECLKKLD 410 TKQQTNTLLN 420 LVWRDSASEE 430 VFTTMASVCH 440 TLMVQHRMMV 450 PKVRLLYSLA 460 FNARFLRHLW 470 FLISSMSTRM 480 ITGSMVPLLQ 490 VISRGSPMSF 500 EDSSRIIPLF 510 YLFSSLFSHS 520 LISIHDNEFF 530 GDPIEVVGQR 540 QSSMMPFTLE 550 ELIMLSRCLR 560 DACLGIIKLA 570 YPETKPEVRE 580 EYITAFQSIG 590 VTTSSEMQQC 600 IQMEQKRWIQ 610 LFKVITNLVK 620 MLKSRDTRRN 630 FCPPNHWLSE 640 QEDIKADKVT 650 QLYVPASRHV 660 WRFRRMGRIG 670 PLQSTLDVGL 680 ESPPLSVSEE 690 RQLAVLTELP 700 FVVPFEERVK 710 IFQRLIYADK 720 QEVQGDGPFL 730 DGINVTIRRN 740 YIYEDAYDKL 750 SPENEPDLKK 760 RIRVHLLNAH 770 GLDEAGIDGG 780 GIFREFLNEL 790 LKSGFNPNQG 800 FFKTTNEGLL 810 YPNPAAQMLV 820 GDSFARHYYF 830 LGRMLGKALY 840 ENMLVELPFA 850 GFFLSKLLGT 860 SADVDIHHLA 870 SLDPEVYKNL 880 LFLKSYEDDV 890 EELGLNFTVV 900 NNDLGEAQVV 910 ELKFGGKDIP 920 VTSANRIAYI 930 HLVADYRLNR 940 QIRQHCLAFR 950 QGLANVVSLE 960 WLRMFDQQEI 970 QVLISGAQVP 980 ISLEDLKSFT 990 NYSGGYSADH 1000 PVIKVFWRVV 1010 EGFTDEEKRK 1020 LLKFVTSCSR 1030 PPLLGFKELY 1040 PAFCIHNGGS 1050 DLERLPTAST 1060 CMNLLKLPEF 1070 YDETLLRSKL 1080 LYAIECAAGF ELS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0000502 proteasome complex
Molecular Function GO:0061630 ubiquitin protein ligase activity
Biological Process GO:0035519 protein K29-linked ubiquitination
Biological Process GO:0070936 protein K48-linked ubiquitination
Biological Process GO:0000209 protein polyubiquitination
Biological Process GO:0006511 ubiquitin-dependent protein catabolic process

Reference

PMID: N/A