Search Results

Overview

Uniprot IDQ15393
Protein NameSplicing factor 3B subunit 3
Gene NameSF3B3
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1049 DTVAGADKFGNICVV
109 IHQETFGKSGCRRIV
137 VMISAIEKQKLVYIL
139 ISAIEKQKLVYILNR
929 GGFVYTYKLVNNGEK

Function

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed:10490618, PubMed:10882114, PubMed:12234937, PubMed:27720643, PubMed:28781166, PubMed:32494006, PubMed:34822310). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed:12234937, PubMed:32494006, PubMed:34822310). Within the 17S U2 SnRNP complex, SF3B3 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA (PubMed:12234937, PubMed:27720643). Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937). May also be involved in the assembly of the 'E' complex (PubMed:10882114). Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs (PubMed:15146077, PubMed:33509932)

Protein Sequence

10 MFLYNLTLQR 20 ATGISFAIHG 30 NFSGTKQQEI 40 VVSRGKILEL 50 LRPDPNTGKV 60 HTLLTVEVFG 70 VIRSLMAFRL 80 TGGTKDYIVV 90 GSDSGRIVIL 100 EYQPSKNMFE 110 KIHQETFGKS 120 GCRRIVPGQF 130 LAVDPKGRAV 140 MISAIEKQKL 150 VYILNRDAAA 160 RLTISSPLEA 170 HKANTLVYHV 180 VGVDVGFENP 190 MFACLEMDYE 200 EADNDPTGEA 210 AANTQQTLTF 220 YELDLGLNHV 230 VRKYSEPLEE 240 HGNFLITVPG 250 GSDGPSGVLI 260 CSENYITYKN 270 FGDQPDIRCP 280 IPRRRNDLDD 290 PERGMIFVCS 300 ATHKTKSMFF 310 FLAQTEQGDI 320 FKITLETDED 330 MVTEIRLKYF 340 DTVPVAAAMC 350 VLKTGFLFVA 360 SEFGNHYLYQ 370 IAHLGDDDEE 380 PEFSSAMPLE 390 EGDTFFFQPR 400 PLKNLVLVDE 410 LDSLSPILFC 420 QIADLANEDT 430 PQLYVACGRG 440 PRSSLRVLRH 450 GLEVSEMAVS 460 ELPGNPNAVW 470 TVRRHIEDEF 480 DAYIIVSFVN 490 ATLVLSIGET 500 VEEVTDSGFL 510 GTTPTLSCSL 520 LGDDALVQVY 530 PDGIRHIRAD 540 KRVNEWKTPG 550 KKTIVKCAVN 560 QRQVVIALTG 570 GELVYFEMDP 580 SGQLNEYTER 590 KEMSADVVCM 600 SLANVPPGEQ 610 RSRFLAVGLV 620 DNTVRIISLD 630 PSDCLQPLSM 640 QALPAQPESL 650 CIVEMGGTEK 660 QDELGERGSI 670 GFLYLNIGLQ 680 NGVLLRTVLD 690 PVTGDLSDTR 700 TRYLGSRPVK 710 LFRVRMQGQE 720 AVLAMSSRSW 730 LSYSYQSRFH 740 LTPLSYETLE 750 FASGFASEQC 760 PEGIVAISTN 770 TLRILALEKL 780 GAVFNQVAFP 790 LQYTPRKFVI 800 HPESNNLIII 810 ETDHNAYTEA 820 TKAQRKQQMA 830 EEMVEAAGED 840 ERELAAEMAA 850 AFLNENLPES 860 IFGAPKAGNG 870 QWASVIRVMN 880 PIQGNTLDLV 890 QLEQNEAAFS 900 VAVCRFSNTG 910 EDWYVLVGVA 920 KDLILNPRSV 930 AGGFVYTYKL 940 VNNGEKLEFL 950 HKTPVEEVPA 960 AIAPFQGRVL 970 IGVGKLLRVY 980 DLGKKKLLRK 990 CENKHIANYI 1000 SGIQTIGHRV 1010 IVSDVQESFI 1020 WVRYKRNENQ 1030 LIIFADDTYP 1040 RWVTTASLLD 1050 YDTVAGADKF 1060 GNICVVRLPP 1070 NTNDEVDEDP 1080 TGNKALWDRG 1090 LLNGASQKAE 1100 VIMNYHVGET 1110 VLSLQKTTLI 1120 PGGSESLVYT 1130 TLSGGIGILV 1140 PFTSHEDHDF 1150 FQHVEMHLRS 1160 EHPPLCGRDH 1170 LSFRSYYFPV 1180 KNVIDGDLCE 1190 QFNSMEPNKQ 1200 KNVSEELDRT 1210 PPEVSKKLED IRTRYAF

Gene Ontology

Classification GO ID Description
Cellular Component GO:0071013 catalytic step 2 spliceosome
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005689 U12-type spliceosomal complex
Cellular Component GO:0005686 U2 snRNP
Cellular Component GO:0071005 U2-type precatalytic spliceosome
Cellular Component GO:0005684 U2-type spliceosomal complex
Molecular Function GO:0044877 protein-containing complex binding
Molecular Function GO:0030620 U2 snRNA binding
Biological Process GO:0000398 mRNA splicing, via spliceosome
Biological Process GO:0042177 negative regulation of protein catabolic process
Biological Process GO:0045893 positive regulation of DNA-templated transcription
Biological Process GO:0006282 regulation of DNA repair
Biological Process GO:0043484 regulation of RNA splicing
Biological Process GO:0008380 RNA splicing
Biological Process GO:0000375 RNA splicing, via transesterification reactions
Biological Process GO:0000387 spliceosomal snRNP assembly
Biological Process GO:0000388 spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)
Biological Process GO:1903241 U2-type prespliceosome assembly

Reference

[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[2] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.