Overview
| Uniprot ID | Q15427 |
|---|
| Protein Name | Splicing factor 3B subunit 4 |
|---|
| Gene Name | SF3B4 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 82 |
NMIKLYGKPIRVNKA |
Function
Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed:10882114, PubMed:12234937, PubMed:27720643, PubMed:32494006). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed:12234937, PubMed:32494006). Within the 17S U2 SnRNP complex, SF3B4 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA (PubMed:12234937, PubMed:27720643). Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937). May also be involved in the assembly of the 'E' complex (PubMed:10882114). Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs (PubMed:15146077, PubMed:33509932)
Protein Sequence
10
MAAGPISERN
20
QDATVYVGGL
30
DEKVSEPLLW
40
ELFLQAGPVV
50
NTHMPKDRVT
60
GQHQGYGFVE
70
FLSEEDADYA
80
IKIMNMIKLY
90
GKPIRVNKAS
100
AHNKNLDVGA
110
NIFIGNLDPE
120
IDEKLLYDTF
130
SAFGVILQTP
140
KIMRDPDTGN
150
SKGYAFINFA
160
SFDASDAAIE
170
AMNGQYLCNR
180
PITVSYAFKK
190
DSKGERHGSA
200
AERLLAAQNP
210
LSQADRPHQL
220
FADAPPPPSA
230
PNPVVSSLGS
240
GLPPPGMPPP
250
GSFPPPVPPP
260
GALPPGIPPA
270
MPPPPMPPGA
280
AGHGPPSAGT
290
PGAGHPGHGH
300
SHPHPFPPGG
310
MPHPGMSQMQ
320
LAHHGPHGLG
330
HPHAGPPGSG
340
GQPPPRPPPG
350
MPHPGPPPMG
360
MPPRGPPFGS
370
PMGHPGPMPP
380
HGMRGPPPLM
390
PPHGYTGPPR
400
PPPYGYQRGP
410
LPPPRPTPRP
420
PVPPRGPLRG
PLPQ
Gene Ontology
| Classification |
GO ID |
Description |
| Molecular Function |
GO:0003723 |
RNA binding |
| Molecular Function |
GO:1990935 |
splicing factor binding |
| Biological Process |
GO:0006397 |
mRNA processing |
| Biological Process |
GO:0000398 |
mRNA splicing, via spliceosome |
| Biological Process |
GO:0008380 |
RNA splicing |
| Biological Process |
GO:0000375 |
RNA splicing, via transesterification reactions |
| Biological Process |
GO:0000387 |
spliceosomal snRNP assembly |
| Biological Process |
GO:0000388 |
spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) |
| Biological Process |
GO:1903241 |
U2-type prespliceosome assembly |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0071011 |
precatalytic spliceosome |
| Cellular Component |
GO:0005681 |
spliceosomal complex |
| Cellular Component |
GO:0005689 |
U12-type spliceosomal complex |
| Cellular Component |
GO:0005686 |
U2 snRNP |
| Cellular Component |
GO:0071005 |
U2-type precatalytic spliceosome |
| Cellular Component |
GO:0005684 |
U2-type spliceosomal complex |
Reference
[1] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.