Search Results

Overview

Uniprot IDQ15477
Protein NameSuperkiller complex protein 2
Gene NameSKIC2
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
531 AKKERMSKHAQTFGA

Function

Helicase component of the SKI complex, a multiprotein complex that assists the RNA-degrading exosome during the mRNA decay and quality-control pathways (PubMed:16024656, PubMed:32006463, PubMed:35120588). The SKI complex catalyzes mRNA extraction from 80S ribosomal complexes in the 3'-5' direction and channels mRNA to the cytosolic exosome for degradation (PubMed:32006463, PubMed:35120588). SKI-mediated extraction of mRNA from stalled ribosomes allow binding of the Pelota-HBS1L complex and subsequent ribosome disassembly by ABCE1 for ribosome recycling (PubMed:32006463). In the nucleus, the SKI complex associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) (PubMed:16024656). May play a role in the non-stop mRNA decay pathway (NSD), which specifically targets and degrades mRNAs that lack a proper stop codon (PubMed:40441874)

Protein Sequence

10 MMETERLVLP 20 PPDPLDLPLR 30 AVELGCTGHW 40 ELLNLPGAPE 50 SSLPHGLPPC 60 APDLQQEAEQ 70 LFLSSPAWLP 80 LHGVEHSARK 90 WQRKTDPWSL 100 LAVLGAPVPS 110 DLQAQRHPTT 120 GQILGYKEVL 130 LENTNLSATT 140 SLSLRRPPGP 150 ASQSLWGNPT 160 QYPFWPGGMD 170 EPTITDLNTR 180 EEAEEEIDFE 190 KDLLTIPPGF 200 KKGMDFAPKD 210 CPTPAPGLLS 220 LSCMLEPLDL 230 GGGDEDENEA 240 VGQPGGPRGD 250 TVSASPCSAP 260 LARASSLEDL 270 VLKEASTAVS 280 TPEAPEPPSQ 290 EQWAIPVDAT 300 SPVGDFYRLI 310 PQPAFQWAFE 320 PDVFQKQAIL 330 HLERHDSVFV 340 AAHTSAGKTV 350 VAEYAIALAQ 360 KHMTRTIYTS 370 PIKALSNQKF 380 RDFRNTFGDV 390 GLLTGDVQLH 400 PEASCLIMTT 410 EILRSMLYSG 420 SDVIRDLEWV 430 IFDEVHYIND 440 VERGVVWEEV 450 LIMLPDHVSI 460 ILLSATVPNA 470 LEFADWIGRL 480 KRRQIYVIST 490 VTRPVPLEHY 500 LFTGNSSKTQ 510 GELFLLLDSR 520 GAFHTKGYYA 530 AVEAKKERMS 540 KHAQTFGAKQ 550 PTHQGGPAQD 560 RGVYLSLLAS 570 LRTRAQLPVV 580 VFTFSRGRCD 590 EQASGLTSLD 600 LTTSSEKSEI 610 HLFLQRCLAR 620 LRGSDRQLPQ 630 VLHMSELLNR 640 GLGVHHSGIL 650 PILKEIVEML 660 FSRGLVKVLF 670 ATETFAMGVN 680 MPARTVVFDS 690 MRKHDGSTFR 700 DLLPGEYVQM 710 AGRAGRRGLD 720 PTGTVILLCK 730 GRVPEMADLH 740 RMMMGKPSQL 750 QSQFRLTYTM 760 ILNLLRVDAL 770 RVEDMMKRSF 780 SEFPSRKDSK 790 AHEQALAELT 800 KRLGALEEPD 810 MTGQLVDLPE 820 YYSWGEELTE 830 TQHMIQRRIM 840 ESVNGLKSLS 850 AGRVVVVKNQ 860 EHHNALGVIL 870 QVSSNSTSRV 880 FTTLVLCDKP 890 LSQDPQDRGP 900 ATAEVPYPDD 910 LVGFKLFLPE 920 GPCDHTVVKL 930 QPGDMAAITT 940 KVLRVNGEKI 950 LEDFSKRQQP 960 KFKKDPPLAA 970 VTTAVQELLR 980 LAQAHPAGPP 990 TLDPVNDLQL 1000 KDMSVVEGGL 1010 RARKLEELIQ 1020 GAQCVHSPRF 1030 PAQYLKLRER 1040 MQIQKEMERL 1050 RFLLSDQSLL 1060 LLPEYHQRVE 1070 VLRTLGYVDE 1080 AGTVKLAGRV 1090 ACAMSSHELL 1100 LTELMFDNAL 1110 STLRPEEIAA 1120 LLSGLVCQSP 1130 GDAGDQLPNT 1140 LKQGIERVRA 1150 VAKRIGEVQV 1160 ACGLNQTVEE 1170 FVGELNFGLV 1180 EVVYEWARGM 1190 PFSELAGLSG 1200 TPEGLVVRCI 1210 QRLAEMCRSL 1220 RGAARLVGEP 1230 VLGAKMETAA 1240 TLLRRDIVFA ASLYTQ

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005634 nucleus
Cellular Component GO:0055087 Ski complex
Molecular Function GO:0034458 3'-5' RNA helicase activity
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0003724 RNA helicase activity
Biological Process GO:0000956 nuclear-transcribed mRNA catabolic process
Biological Process GO:0070478 nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay
Biological Process GO:0070481 nuclear-transcribed mRNA catabolic process, non-stop decay
Biological Process GO:0072344 rescue of stalled cytosolic ribosome

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.