Overview
| Uniprot ID | Q15629 |
|---|
| Protein Name | Translocating chain-associated membrane protein 1 |
|---|
| Gene Name | TRAM1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 335 |
AFQAPAVKKKPTVTK |
Function
Involved in the translocation of nascent protein chains into or through the endoplasmic reticulum (ER) membrane by facilitating the proper chain positioning at the SEC61 channel (PubMed:12475939, PubMed:1315422, PubMed:32013668, PubMed:8616892, PubMed:9506517). Regulates the exposure of nascent secretory protein chain to the cytosol during translocation into the ER (PubMed:9506517). May affect the phospholipid bilayer in the vicinity of the lateral gate of the SEC61 channel, thereby facilitating ER protein transport (PubMed:32013668). Intimately associates with transmembrane (TM) domain of nascent membrane proteins during the entire integration process into the ER membrane (PubMed:8616892). Associates with the second TM domain of G protein-coupled receptor opsin/OPSD nascent chain in the ER membrane, which may facilitate its integration into the membrane (PubMed:12475939). Under conditions of ER stress, participates in the disposal of misfolded ER membrane proteins during the unfolded protein response (UPR), an integrated stress response (ISR) pathway, by selectively retrotranslocating misfolded ER-membrane proteins from the ER into the cytosol where they are ubiquitinated and degraded by the proteasome (PubMed:20430023)
Protein Sequence
10
MAIRKKSTKS
20
PPVLSHEFVL
30
QNHADIVSCV
40
AMVFLLGLMF
50
EITAKASIIF
60
VTLQYNVTLP
70
ATEEQATESV
80
SLYYYGIKDL
90
ATVFFYMLVA
100
IIIHAVIQEY
110
MLDKINRRMH
120
FSKTKHSKFN
130
ESGQLSAFYL
140
FACVWGTFIL
150
ISENYISDPT
160
ILWRAYPHNL
170
MTFQMKFFYI
180
SQLAYWLHAF
190
PELYFQKTKK
200
EDIPRQLVYI
210
GLYLFHIAGA
220
YLLNLNHLGL
230
VLLVLHYFVE
240
FLFHISRLFY
250
FSNEKYQKGF
260
SLWAVLFVLG
270
RLLTLILSVL
280
TVGFGLARAE
290
NQKLDFSTGN
300
FNVLAVRIAV
310
LASICVTQAF
320
MMWKFINFQL
330
RRWREHSAFQ
340
APAVKKKPTV
350
TKGRSSKKGT
360
ENGVNGTLTS
370
NVADSPRNKK
EKSS
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005783 |
endoplasmic reticulum |
| Cellular Component |
GO:0005789 |
endoplasmic reticulum membrane |
| Cellular Component |
GO:0016020 |
membrane |
| Molecular Function |
GO:0038023 |
signaling receptor activity |
| Biological Process |
GO:0006613 |
cotranslational protein targeting to membrane |
| Biological Process |
GO:0045048 |
protein insertion into ER membrane |
| Biological Process |
GO:0006986 |
response to unfolded protein |
| Biological Process |
GO:0006616 |
SRP-dependent cotranslational protein targeting to membrane, translocation |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.