Search Results

Overview

Uniprot IDQ15637
Protein NameSplicing factor 1
Gene NameSF1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
15 TPLDFPSKKRKRSRW
16 PLDFPSKKRKRSRWN
174 IEKECNAKIMIRGKG
191 KEGKVGRKDGQMLPG
293 GHIASDCKFQRPGDP
306 DPQSAQDKARMDKEY

Function

Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor

Protein Sequence

10 MATGANATPL 20 DFPSKKRKRS 30 RWNQDTMEQK 40 TVIPGMPTVI 50 PPGLTREQER 60 AYIVQLQIED 70 LTRKLRTGDL 80 GIPPNPEDRS 90 PSPEPIYNSE 100 GKRLNTREFR 110 TRKKLEEERH 120 NLITEMVALN 130 PDFKPPADYK 140 PPATRVSDKV 150 MIPQDEYPEI 160 NFVGLLIGPR 170 GNTLKNIEKE 180 CNAKIMIRGK 190 GSVKEGKVGR 200 KDGQMLPGED 210 EPLHALVTAN 220 TMENVKKAVE 230 QIRNILKQGI 240 ETPEDQNDLR 250 KMQLRELARL 260 NGTLREDDNR 270 ILRPWQSSET 280 RSITNTTVCT 290 KCGGAGHIAS 300 DCKFQRPGDP 310 QSAQDKARMD 320 KEYLSLMAEL 330 GEAPVPASVG 340 STSGPATTPL 350 ASAPRPAAPA 360 NNPPPPSLMS 370 TTQSRPPWMN 380 SGPSESRPYH 390 GMHGGGPGGP 400 GGGPHSFPHP 410 LPSLTGGHGG 420 HPMQHNPNGP 430 PPPWMQPPPP 440 PMNQGPHPPG 450 HHGPPPMDQY 460 LGSTPVGSGV 470 YRLHQGKGMM 480 PPPPMGMMPP 490 PPPPPSGQPP 500 PPPSGPLPPW 510 QQQQQQPPPP 520 PPPSSSMASS 530 TPLPWQQNTT 540 TTTTSAGTGS 550 IPPWQQQQAA 560 AAASPGAPQM 570 QGNPTMVPLP 580 PGVQPPLPPG 590 APPPPPPPPP 600 GSAGMMYAPP 610 PPPPPPMDPS 620 NFVTMMGMGV 630 AGMPPFGMPP APPPPPPQN

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005840 ribosome
Cellular Component GO:0005681 spliceosomal complex
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0003729 mRNA binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0003714 transcription corepressor activity
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0000389 mRNA 3'-splice site recognition
Biological Process GO:0045292 mRNA cis splicing, via spliceosome
Biological Process GO:0000398 mRNA splicing, via spliceosome
Biological Process GO:0000245 spliceosomal complex assembly

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[5] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[6] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[7] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[8] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[9] Chao L, Xu Y, Yang Y, Ao X, Liang J. Identification of lactylation-related biomarkers for diagnosis, prognosis, and treatment responsiveness in triple-negative breast cancer.. World J Surg Oncol 24(1):77. 2026 Jan 22. PMID: 41566505.

[10] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.