Search Results
Overview
| Uniprot ID | Q15691 |
|---|---|
| Protein Name | Microtubule-associated protein RP/EB family member 1 |
| Gene Name | MAPRE1 |
| Organism | Homo sapiens |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 113 | EFVQWFKKFFDANYD |
| 148 | LVAPALNKPKKPLTS |
| 150 | APALNKPKKPLTSSS |
| 151 | PALNKPKKPLTSSSA |
| 174 | QRTAAAPKAGPGVVR |
| 182 | AGPGVVRKNPGVGNG |
| 83 | KILQAGFKRMGVDKI |
Function
Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton (PubMed:12388762, PubMed:16109370, PubMed:19632184, PubMed:21646404, PubMed:23001180, PubMed:28726242, PubMed:28814570, PubMed:34608293). Recruits other +TIP proteins to microtubules by binding to a conserved Ser-X-Leu-Pro (SXLP) motif in their polypeptide chains (PubMed:19632184, PubMed:36592928). Promotes cytoplasmic microtubule nucleation and elongation (PubMed:12388762, PubMed:16109370, PubMed:19632184, PubMed:21646404, PubMed:28726242, PubMed:28814570). Involved in mitotic spindle positioning by stabilizing microtubules and promoting dynamic connection between astral microtubules and the cortex during mitotic chromosome segregation (PubMed:12388762, PubMed:34608293). Assists chromosome alignment in metaphase by recruiting the SKA complex to the spindle and stabilizing its interactions with microtubule bundles (K-fibers) (PubMed:27225956, PubMed:36592928). Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:28814570). Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (PubMed:28814570). Acts as a regulator of autophagosome transport via interaction with CAMSAP2 (PubMed:28726242). Functions downstream of Rho GTPases and DIAPH1 in stable microtubule formation (By similarity). Promotes microtubule-mediated GJA1/CX43 trafficking to cell membranes via its interaction with DSP, thereby facilitating gap junction intracellular communication (PubMed:25225338). May play a role in cell migration (By similarity)
Protein Sequence
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Cellular Component | GO:0036064 | ciliary basal body |
| Cellular Component | GO:0031253 | cell projection membrane |
| Cellular Component | GO:0005813 | centrosome |
| Cellular Component | GO:0030981 | cortical microtubule cytoskeleton |
| Cellular Component | GO:0005881 | cytoplasmic microtubule |
| Cellular Component | GO:0005829 | cytosol |
| Cellular Component | GO:0005925 | focal adhesion |
| Cellular Component | GO:0005794 | Golgi apparatus |
| Cellular Component | GO:0005874 | microtubule |
| Cellular Component | GO:0005815 | microtubule organizing center |
| Cellular Component | GO:0035371 | microtubule plus-end |
| Cellular Component | GO:1905721 | mitotic spindle astral microtubule end |
| Cellular Component | GO:1990498 | mitotic spindle microtubule |
| Cellular Component | GO:0097431 | mitotic spindle pole |
| Cellular Component | GO:0051233 | spindle midzone |
| Molecular Function | GO:0045296 | cadherin binding |
| Molecular Function | GO:0042802 | identical protein binding |
| Molecular Function | GO:0051010 | microtubule plus-end binding |
| Molecular Function | GO:0120283 | protein serine/threonine kinase binding |
| Molecular Function | GO:0003723 | RNA binding |
| Biological Process | GO:0051315 | attachment of mitotic spindle microtubules to kinetochore |
| Biological Process | GO:0051301 | cell division |
| Biological Process | GO:0016477 | cell migration |
| Biological Process | GO:0000132 | establishment of mitotic spindle orientation |
| Biological Process | GO:0008104 | intracellular protein localization |
| Biological Process | GO:0001578 | microtubule bundle formation |
| Biological Process | GO:0046785 | microtubule polymerization |
| Biological Process | GO:0031115 | negative regulation of microtubule polymerization |
| Biological Process | GO:0031116 | positive regulation of microtubule polymerization |
| Biological Process | GO:1902888 | protein localization to astral microtubule |
| Biological Process | GO:0035372 | protein localization to microtubule |
| Biological Process | GO:1902480 | protein localization to mitotic spindle |
| Biological Process | GO:0031110 | regulation of microtubule polymerization or depolymerization |
| Biological Process | GO:0051225 | spindle assembly |
Reference
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[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.
[4] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.
[5] Yang YH, Wang QC, Kong J, Yang JT, Liu JF. Global profiling of lysine lactylation in human lungs.. Proteomics 23(15):e2200437. 2023 Aug. PMID: 37170646.
[6] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.
[7] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.
[8] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.
[9] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.
[10] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.