Search Results

Overview

Uniprot ID	Q15811
Protein Name	Intersectin-1
Gene Name	ITSN1
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
197	PGSQLNTKLQKAQSF
704	GKQEAQDKLGRLFHQ

Function

Adapter protein that provides a link between the endocytic membrane traffic and the actin assembly machinery (PubMed:11584276, PubMed:29887380). Acts as a guanine nucleotide exchange factor (GEF) for CDC42, and thereby stimulates actin nucleation mediated by WASL and the ARP2/3 complex (PubMed:11584276). Plays a role in the assembly and maturation of clathrin-coated vesicles (By similarity). Recruits FCHSD2 to clathrin-coated pits (PubMed:29887380). Involved in endocytosis of activated EGFR, and probably also other growth factor receptors (By similarity). Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2 (PubMed:22648170). Promotes ubiquitination and subsequent degradation of EGFR, and thereby contributes to the down-regulation of EGFR-dependent signaling pathways. In chromaffin cells, required for normal exocytosis of catecholamines. Required for rapid replenishment of release-ready synaptic vesicles at presynaptic active zones (By similarity). Inhibits ARHGAP31 activity toward RAC1 (PubMed:11744688)

Protein Sequence

10 MAQFPTPFGG 20 SLDIWAITVE 30 ERAKHDQQFH 40 SLKPISGFIT 50 GDQARNFFFQ 60 SGLPQPVLAQ 70 IWALADMNND 80 GRMDQVEFSI 90 AMKLIKLKLQ 100 GYQLPSALPP 110 VMKQQPVAIS 120 SAPAFGMGGI 130 ASMPPLTAVA 140 PVPMGSIPVV 150 GMSPTLVSSV 160 PTAAVPPLAN 170 GAPPVIQPLP 180 AFAHPAATLP 190 KSSSFSRSGP 200 GSQLNTKLQK 210 AQSFDVASVP 220 PVAEWAVPQS 230 SRLKYRQLFN 240 SHDKTMSGHL 250 TGPQARTILM 260 QSSLPQAQLA 270 SIWNLSDIDQ 280 DGKLTAEEFI 290 LAMHLIDVAM 300 SGQPLPPVLP 310 PEYIPPSFRR 320 VRSGSGISVI 330 SSTSVDQRLP 340 EEPVLEDEQQ 350 QLEKKLPVTF 360 EDKKRENFER 370 GNLELEKRRQ 380 ALLEQQRKEQ 390 ERLAQLERAE 400 QERKERERQE 410 QERKRQLELE 420 KQLEKQRELE 430 RQREEERRKE 440 IERREAAKRE 450 LERQRQLEWE 460 RNRRQELLNQ 470 RNKEQEDIVV 480 LKAKKKTLEF 490 ELEALNDKKH 500 QLEGKLQDIR 510 CRLTTQRQEI 520 ESTNKSRELR 530 IAEITHLQQQ 540 LQESQQMLGR 550 LIPEKQILND 560 QLKQVQQNSL 570 HRDSLVTLKR 580 ALEAKELARQ 590 HLRDQLDEVE 600 KETRSKLQEI 610 DIFNNQLKEL 620 REIHNKQQLQ 630 KQKSMEAERL 640 KQKEQERKII 650 ELEKQKEEAQ 660 RRAQERDKQW 670 LEHVQQEDEH 680 QRPRKLHEEE 690 KLKREESVKK 700 KDGEEKGKQE 710 AQDKLGRLFH 720 QHQEPAKPAV 730 QAPWSTAEKG 740 PLTISAQENV 750 KVVYYRALYP 760 FESRSHDEIT 770 IQPGDIVMVK 780 GEWVDESQTG 790 EPGWLGGELK 800 GKTGWFPANY 810 AEKIPENEVP 820 APVKPVTDST 830 SAPAPKLALR 840 ETPAPLAVTS 850 SEPSTTPNNW 860 ADFSSTWPTS 870 TNEKPETDNW 880 DAWAAQPSLT 890 VPSAGQLRQR 900 SAFTPATATG 910 SSPSPVLGQG 920 EKVEGLQAQA 930 LYPWRAKKDN 940 HLNFNKNDVI 950 TVLEQQDMWW 960 FGEVQGQKGW 970 FPKSYVKLIS 980 GPIRKSTSMD 990 SGSSESPASL 1000 KRVASPAAKP 1010 VVSGEEFIAM 1020 YTYESSEQGD 1030 LTFQQGDVIL 1040 VTKKDGDWWT 1050 GTVGDKAGVF 1060 PSNYVRLKDS 1070 EGSGTAGKTG 1080 SLGKKPEIAQ 1090 VIASYTATGP 1100 EQLTLAPGQL 1110 ILIRKKNPGG 1120 WWEGELQARG 1130 KKRQIGWFPA 1140 NYVKLLSPGT 1150 SKITPTEPPK 1160 STALAAVCQV 1170 IGMYDYTAQN 1180 DDELAFNKGQ 1190 IINVLNKEDP 1200 DWWKGEVNGQ 1210 VGLFPSNYVK 1220 LTTDMDPSQQ 1230 WCSDLHLLDM 1240 LTPTERKRQG 1250 YIHELIVTEE 1260 NYVNDLQLVT 1270 EIFQKPLMES 1280 ELLTEKEVAM 1290 IFVNWKELIM 1300 CNIKLLKALR 1310 VRKKMSGEKM 1320 PVKMIGDILS 1330 AQLPHMQPYI 1340 RFCSRQLNGA 1350 ALIQQKTDEA 1360 PDFKEFVKRL 1370 AMDPRCKGMP 1380 LSSFILKPMQ 1390 RVTRYPLIIK 1400 NILENTPENH 1410 PDHSHLKHAL 1420 EKAEELCSQV 1430 NEGVREKENS 1440 DRLEWIQAHV 1450 QCEGLSEQLV 1460 FNSVTNCLGP 1470 RKFLHSGKLY 1480 KAKSNKELYG 1490 FLFNDFLLLT 1500 QITKPLGSSG 1510 TDKVFSPKSN 1520 LQYKMYKTPI 1530 FLNEVLVKLP 1540 TDPSGDEPIF 1550 HISHIDRVYT 1560 LRAESINERT 1570 AWVQKIKAAS 1580 ELYIETEKKK 1590 REKAYLVRSQ 1600 RATGIGRLMV 1610 NVVEGIELKP 1620 CRSHGKSNPY 1630 CEVTMGSQCH 1640 ITKTIQDTLN 1650 PKWNSNCQFF 1660 IRDLEQEVLC 1670 ITVFERDQFS 1680 PDDFLGRTEI 1690 RVADIKKDQG 1700 SKGPVTKCLL 1710 LHEVPTGEIV 1720 VRLDLQLFDE P

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0097440	apical dendrite
Cellular Component	GO:0098978	glutamatergic synapse
Cellular Component	GO:0097708	intracellular vesicle
Cellular Component	GO:0030027	lamellipodium
Cellular Component	GO:0043025	neuronal cell body
Cellular Component	GO:0005635	nuclear envelope
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005886	plasma membrane
Cellular Component	GO:0098871	postsynaptic actin cytoskeleton
Cellular Component	GO:0098843	postsynaptic endocytic zone
Cellular Component	GO:0098793	presynapse
Cellular Component	GO:0055037	recycling endosome
Molecular Function	GO:0005509	calcium ion binding
Molecular Function	GO:0005085	guanyl-nucleotide exchange factor activity
Molecular Function	GO:0060090	molecular adaptor activity
Molecular Function	GO:0070064	proline-rich region binding
Molecular Function	GO:0030674	protein-macromolecule adaptor activity
Biological Process	GO:0150007	clathrin-dependent synaptic vesicle endocytosis
Biological Process	GO:0016197	endosomal transport
Biological Process	GO:0006887	exocytosis
Biological Process	GO:0008104	intracellular protein localization
Biological Process	GO:0035556	intracellular signal transduction
Biological Process	GO:2001288	positive regulation of caveolin-mediated endocytosis
Biological Process	GO:0060999	positive regulation of dendritic spine development
Biological Process	GO:0060124	positive regulation of growth hormone secretion
Biological Process	GO:0015031	protein transport
Biological Process	GO:1905274	regulation of modification of postsynaptic actin cytoskeleton
Biological Process	GO:0099175	regulation of postsynapse organization
Biological Process	GO:0051056	regulation of small GTPase mediated signal transduction
Cellular Component	GO:0005905	clathrin-coated pit
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0043197	dendritic spine

Reference

[1] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[2] Chao L, Xu Y, Yang Y, Ao X, Liang J. Identification of lactylation-related biomarkers for diagnosis, prognosis, and treatment responsiveness in triple-negative breast cancer.. World J Surg Oncol 24(1):77. 2026 Jan 22. PMID: 41566505.