Overview
| Uniprot ID | Q16531 |
|---|
| Protein Name | DNA damage-binding protein 1 |
|---|
| Gene Name | DDB1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 11 |
NYVVTAQKPTAVNGC |
| 383 |
VTCSGAFKEGSLRII |
| 723 |
PLYESPRKICYQEVS |
Function
Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively (PubMed:14739464, PubMed:15448697, PubMed:16260596, PubMed:16407242, PubMed:16407252, PubMed:16482215, PubMed:16940174, PubMed:17079684, PubMed:25970626). Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair (PubMed:15448697, PubMed:16260596, PubMed:16407242, PubMed:16940174). The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches (PubMed:15448697, PubMed:16260596, PubMed:16407242, PubMed:16940174). Also functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:14739464, PubMed:16407252, PubMed:16482215, PubMed:17079684, PubMed:18332868, PubMed:18381890, PubMed:19966799, PubMed:22118460, PubMed:25043012, PubMed:25108355, PubMed:28886238). The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1 (PubMed:14739464, PubMed:16407252, PubMed:16482215, PubMed:17079684, PubMed:18332868, PubMed:18381890, PubMed:19966799, PubMed:22118460, PubMed:25043012, PubMed:25108355). DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage (PubMed:16473935, PubMed:16678110, PubMed:17041588, PubMed:18593899). The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair (PubMed:16473935, PubMed:16678110, PubMed:17041588, PubMed:18593899). DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER (PubMed:15882621). DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication (PubMed:17041588). DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR) (PubMed:12732143, PubMed:32355176, PubMed:38316879). The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (PubMed:26431207). DDB1-mediated CRY1 degradation promotes FOXO1 protein stability and FOXO1-mediated gluconeogenesis in the liver (By similarity). By acting on TET dioxygenses, essential for oocyte maintenance at the primordial follicle stage, hence essential for female fertility (By similarity). Maternal factor required for proper zygotic genome activation and genome reprogramming (By similarity)
Protein Sequence
10
MSYNYVVTAQ
20
KPTAVNGCVT
30
GHFTSAEDLN
40
LLIAKNTRLE
50
IYVVTAEGLR
60
PVKEVGMYGK
70
IAVMELFRPK
80
GESKDLLFIL
90
TAKYNACILE
100
YKQSGESIDI
110
ITRAHGNVQD
120
RIGRPSETGI
130
IGIIDPECRM
140
IGLRLYDGLF
150
KVIPLDRDNK
160
ELKAFNIRLE
170
ELHVIDVKFL
180
YGCQAPTICF
190
VYQDPQGRHV
200
KTYEVSLREK
210
EFNKGPWKQE
220
NVEAEASMVI
230
AVPEPFGGAI
240
IIGQESITYH
250
NGDKYLAIAP
260
PIIKQSTIVC
270
HNRVDPNGSR
280
YLLGDMEGRL
290
FMLLLEKEEQ
300
MDGTVTLKDL
310
RVELLGETSI
320
AECLTYLDNG
330
VVFVGSRLGD
340
SQLVKLNVDS
350
NEQGSYVVAM
360
ETFTNLGPIV
370
DMCVVDLERQ
380
GQGQLVTCSG
390
AFKEGSLRII
400
RNGIGIHEHA
410
SIDLPGIKGL
420
WPLRSDPNRE
430
TDDTLVLSFV
440
GQTRVLMLNG
450
EEVEETELMG
460
FVDDQQTFFC
470
GNVAHQQLIQ
480
ITSASVRLVS
490
QEPKALVSEW
500
KEPQAKNISV
510
ASCNSSQVVV
520
AVGRALYYLQ
530
IHPQELRQIS
540
HTEMEHEVAC
550
LDITPLGDSN
560
GLSPLCAIGL
570
WTDISARILK
580
LPSFELLHKE
590
MLGGEIIPRS
600
ILMTTFESSH
610
YLLCALGDGA
620
LFYFGLNIET
630
GLLSDRKKVT
640
LGTQPTVLRT
650
FRSLSTTNVF
660
ACSDRPTVIY
670
SSNHKLVFSN
680
VNLKEVNYMC
690
PLNSDGYPDS
700
LALANNSTLT
710
IGTIDEIQKL
720
HIRTVPLYES
730
PRKICYQEVS
740
QCFGVLSSRI
750
EVQDTSGGTT
760
ALRPSASTQA
770
LSSSVSSSKL
780
FSSSTAPHET
790
SFGEEVEVHN
800
LLIIDQHTFE
810
VLHAHQFLQN
820
EYALSLVSCK
830
LGKDPNTYFI
840
VGTAMVYPEE
850
AEPKQGRIVV
860
FQYSDGKLQT
870
VAEKEVKGAV
880
YSMVEFNGKL
890
LASINSTVRL
900
YEWTTEKELR
910
TECNHYNNIM
920
ALYLKTKGDF
930
ILVGDLMRSV
940
LLLAYKPMEG
950
NFEEIARDFN
960
PNWMSAVEIL
970
DDDNFLGAEN
980
AFNLFVCQKD
990
SAATTDEERQ
1000
HLQEVGLFHL
1010
GEFVNVFCHG
1020
SLVMQNLGET
1030
STPTQGSVLF
1040
GTVNGMIGLV
1050
TSLSESWYNL
1060
LLDMQNRLNK
1070
VIKSVGKIEH
1080
SFWRSFHTER
1090
KTEPATGFID
1100
GDLIESFLDI
1110
SRPKMQEVVA
1120
NLQYDDGSGM
1130
KREATADDLI
1140
KVVEELTRIH
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0000781 |
chromosome, telomeric region |
| Molecular Function |
GO:0030674 |
protein-macromolecule adaptor activity |
| Molecular Function |
GO:0160072 |
ubiquitin ligase complex scaffold activity |
| Molecular Function |
GO:0071987 |
WD40-repeat domain binding |
| Biological Process |
GO:0051702 |
biological process involved in interaction with symbiont |
| Biological Process |
GO:0008283 |
cell population proliferation |
| Biological Process |
GO:0034644 |
cellular response to UV |
| Biological Process |
GO:0006974 |
DNA damage response |
| Biological Process |
GO:0006281 |
DNA repair |
| Biological Process |
GO:0044725 |
epigenetic programming in the zygotic pronuclei |
| Biological Process |
GO:0040029 |
epigenetic regulation of gene expression |
| Biological Process |
GO:1904178 |
negative regulation of adipose tissue development |
| Biological Process |
GO:0006289 |
nucleotide-excision repair |
| Biological Process |
GO:0046726 |
positive regulation by virus of viral protein levels in host cell |
| Biological Process |
GO:0045722 |
positive regulation of gluconeogenesis |
| Biological Process |
GO:0045732 |
positive regulation of protein catabolic process |
| Biological Process |
GO:0045070 |
positive regulation of viral genome replication |
| Biological Process |
GO:0010498 |
proteasomal protein catabolic process |
| Biological Process |
GO:0043161 |
proteasome-mediated ubiquitin-dependent protein catabolic process |
| Biological Process |
GO:0016567 |
protein ubiquitination |
| Biological Process |
GO:0042981 |
regulation of apoptotic process |
| Biological Process |
GO:0010506 |
regulation of autophagy |
| Biological Process |
GO:1901987 |
regulation of cell cycle phase transition |
| Biological Process |
GO:0042127 |
regulation of cell population proliferation |
| Biological Process |
GO:0080135 |
regulation of cellular response to stress |
| Biological Process |
GO:0042752 |
regulation of circadian rhythm |
| Biological Process |
GO:0030174 |
regulation of DNA-templated DNA replication initiation |
| Biological Process |
GO:0045995 |
regulation of embryonic development |
| Biological Process |
GO:0060964 |
regulation of miRNA-mediated gene silencing |
| Biological Process |
GO:1901990 |
regulation of mitotic cell cycle phase transition |
| Biological Process |
GO:1902412 |
regulation of mitotic cytokinesis |
| Biological Process |
GO:0032814 |
regulation of natural killer cell activation |
| Biological Process |
GO:2000036 |
regulation of stem cell population maintenance |
| Biological Process |
GO:0031297 |
replication fork processing |
| Biological Process |
GO:0048511 |
rhythmic process |
| Biological Process |
GO:0007283 |
spermatogenesis |
| Biological Process |
GO:0007056 |
spindle assembly involved in female meiosis |
| Biological Process |
GO:0006511 |
ubiquitin-dependent protein catabolic process |
| Biological Process |
GO:0070914 |
UV-damage excision repair |
| Biological Process |
GO:0019076 |
viral release from host cell |
| Cellular Component |
GO:0080008 |
Cul4-RING E3 ubiquitin ligase complex |
| Cellular Component |
GO:0031464 |
Cul4A-RING E3 ubiquitin ligase complex |
| Cellular Component |
GO:0031465 |
Cul4B-RING E3 ubiquitin ligase complex |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0070062 |
extracellular exosome |
| Cellular Component |
GO:0005615 |
extracellular space |
| Cellular Component |
GO:0005730 |
nucleolus |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0032991 |
protein-containing complex |
| Cellular Component |
GO:0035861 |
site of double-strand break |
| Molecular Function |
GO:0097602 |
cullin family protein binding |
| Molecular Function |
GO:0003684 |
damaged DNA binding |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:0044877 |
protein-containing complex binding |
Reference
[1] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.