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Overview

Uniprot IDQ16649
Protein NameNuclear factor interleukin-3-regulated protein
Gene NameNFIL3
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
412 GKTQNSFKTGVVEMK
419 KTGVVEMKDSGYKVS
448 SAEVVSLKRLIATQP
71 REFIPDEKKDAMYWE
72 EFIPDEKKDAMYWEK

Function

Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts (By similarity). Represses transcriptional activity of PER1 (By similarity). Represses transcriptional activity of PER2 via the B-site on the promoter (By similarity). Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF) (By similarity). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock (By similarity). Protects pro-B cells from programmed cell death (By similarity). Represses the transcription of CYP2A5 (By similarity). Positively regulates the expression and activity of CES2 by antagonizing the repressive action of NR1D1 on CES2 (By similarity). Required for the development of natural killer cell precursors (By similarity)

Protein Sequence

10 MQLRKMQTVK 20 KEQASLDASS 30 NVDKMMVLNS 40 ALTEVSEDST 50 TGEELLLSEG 60 SVGKNKSSAC 70 RRKREFIPDE 80 KKDAMYWEKR 90 RKNNEAAKRS 100 REKRRLNDLV 110 LENKLIALGE 120 ENATLKAELL 130 SLKLKFGLIS 140 STAYAQEIQK 150 LSNSTAVYFQ 160 DYQTSKSNVS 170 SFVDEHEPSM 180 VSSSCISVIK 190 HSPQSSLSDV 200 SEVSSVEHTQ 210 ESSVQGSCRS 220 PENKFQIIKQ 230 EPMELESYTR 240 EPRDDRGSYT 250 ASIYQNYMGN 260 SFSGYSHSPP 270 LLQVNRSSSN 280 SPRTSETDDG 290 VVGKSSDGED 300 EQQVPKGPIH 310 SPVELKHVHA 320 TVVKVPEVNS 330 SALPHKLRIK 340 AKAMQIKVEA 350 FDNEFEATQK 360 LSSPIDMTSK 370 RHFELEKHSA 380 PSMVHSSLTP 390 FSVQVTNIQD 400 WSLKSEHWHQ 410 KELSGKTQNS 420 FKTGVVEMKD 430 SGYKVSDPEN 440 LYLKQGIANL 450 SAEVVSLKRL 460 IATQPISASD SG

Gene Ontology

Classification GO ID Description
Cellular Component GO:0000785 chromatin
Cellular Component GO:0005634 nucleus
Molecular Function GO:0003700 DNA-binding transcription factor activity
Molecular Function GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
Molecular Function GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
Molecular Function GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding
Biological Process GO:0007623 circadian rhythm
Biological Process GO:0006955 immune response
Biological Process GO:0001779 natural killer cell differentiation
Biological Process GO:0045892 negative regulation of DNA-templated transcription
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:0045893 positive regulation of DNA-templated transcription
Biological Process GO:0006355 regulation of DNA-templated transcription
Biological Process GO:0006357 regulation of transcription by RNA polymerase II
Biological Process GO:0006366 transcription by RNA polymerase II

Reference

[1] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[2] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.