Overview
| Uniprot ID | Q16763 |
|---|
| Protein Name | Ubiquitin-conjugating enzyme E2 S |
|---|
| Gene Name | UBE2S |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 197 |
GAEGPMAKKHAGERD |
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:19820702, PubMed:19822757, PubMed:22496338, PubMed:27259151). Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis (PubMed:19820702, PubMed:19822757, PubMed:27259151, PubMed:27910872). Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit (PubMed:19820702, PubMed:19822757, PubMed:27259151). Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A (PubMed:16819549). In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination (PubMed:20061386, PubMed:20622874)
Protein Sequence
10
MNSNVENLPP
20
HIIRLVYKEV
30
TTLTADPPDG
40
IKVFPNEEDL
50
TDLQVTIEGP
60
EGTPYAGGLF
70
RMKLLLGKDF
80
PASPPKGYFL
90
TKIFHPNVGA
100
NGEICVNVLK
110
RDWTAELGIR
120
HVLLTIKCLL
130
IHPNPESALN
140
EEAGRLLLEN
150
YEEYAARARL
160
LTEIHGGAGG
170
PSGRAEAGRA
180
LASGTEASST
190
DPGAPGGPGG
200
AEGPMAKKHA
210
GERDKKLAAK
220
KKTDKKRALR
RL
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005680 |
anaphase-promoting complex |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0010997 |
anaphase-promoting complex binding |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0061631 |
ubiquitin conjugating enzyme activity |
| Molecular Function |
GO:0004842 |
ubiquitin-protein transferase activity |
| Biological Process |
GO:0031145 |
anaphase-promoting complex-dependent catabolic process |
| Biological Process |
GO:0051301 |
cell division |
| Biological Process |
GO:0010458 |
exit from mitosis |
| Biological Process |
GO:0010994 |
free ubiquitin chain polymerization |
| Biological Process |
GO:1904668 |
positive regulation of ubiquitin protein ligase activity |
| Biological Process |
GO:0043161 |
proteasome-mediated ubiquitin-dependent protein catabolic process |
| Biological Process |
GO:0070979 |
protein K11-linked ubiquitination |
| Biological Process |
GO:0044314 |
protein K27-linked ubiquitination |
| Biological Process |
GO:0035519 |
protein K29-linked ubiquitination |
| Biological Process |
GO:0085020 |
protein K6-linked ubiquitination |
| Biological Process |
GO:0070534 |
protein K63-linked ubiquitination |
| Biological Process |
GO:0036211 |
protein modification process |
| Biological Process |
GO:0000209 |
protein polyubiquitination |
| Biological Process |
GO:0006511 |
ubiquitin-dependent protein catabolic process |
Reference
PMID: N/A