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Overview

Uniprot IDQ16798
Protein NameNADP-dependent malic enzyme, mitochondrial
Gene NameME3
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
254 KHQRVHGKAYDDLLD
372 KIWMVDSKGLIVKGR
377 DSKGLIVKGRSHLNH
386 RSHLNHEKEMFAQDH
447 ALSNPTSKAECTAEK
454 KAECTAEKCYRVTEG
472 FASGSPFKSVTLEDG
558 KVLDYAYKHNLASYY
569 ASYYPEPKDKEAFVR

Function

Catalyzes the oxidative decarboxylation of (S)-malate to pyruvate using NADP(+), and is able to reverse the reaction but only with significantly lower efficiency (PubMed:7818469). Can also catalyze the decarboxylation of oxaloacetate into pyruvate (By similarity)

Protein Sequence

10 MGAALGTGTR 20 LAPWPGRACG 30 ALPRWTPTAP 40 AQGCHSKPGP 50 ARPVPLKKRG 60 YDVTRNPHLN 70 KGMAFTLEER 80 LQLGIHGLIP 90 PCFLSQDVQL 100 LRIMRYYERQ 110 QSDLDKYIIL 120 MTLQDRNEKL 130 FYRVLTSDVE 140 KFMPIVYTPT 150 VGLACQHYGL 160 TFRRPRGLFI 170 TIHDKGHLAT 180 MLNSWPEDNI 190 KAVVVTDGER 200 ILGLGDLGCY 210 GMGIPVGKLA 220 LYTACGGVNP 230 QQCLPVLLDV 240 GTNNEELLRD 250 PLYIGLKHQR 260 VHGKAYDDLL 270 DEFMQAVTDK 280 FGINCLIQFE 290 DFANANAFRL 300 LNKYRNKYCM 310 FNDDIQGTAS 320 VAVAGILAAL 330 RITKNKLSNH 340 VFVFQGAGEA 350 AMGIAHLLVM 360 ALEKEGVPKA 370 EATRKIWMVD 380 SKGLIVKGRS 390 HLNHEKEMFA 400 QDHPEVNSLE 410 EVVRLVKPTA 420 IIGVAAIAGA 430 FTEQILRDMA 440 SFHERPIIFA 450 LSNPTSKAEC 460 TAEKCYRVTE 470 GRGIFASGSP 480 FKSVTLEDGK 490 TFIPGQGNNA 500 YVFPGVALGV 510 IAGGIRHIPD 520 EIFLLTAEQI 530 AQEVSEQHLS 540 QGRLYPPLST 550 IRDVSLRIAI 560 KVLDYAYKHN 570 LASYYPEPKD 580 KEAFVRSLVY 590 TPDYDSFTLD 600 SYTWPKEAMN VQTV

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0004473 malate dehydrogenase (decarboxylating) (NADP+) activity
Molecular Function GO:0004470 malic enzyme activity
Molecular Function GO:0046872 metal ion binding
Molecular Function GO:0051287 NAD binding
Molecular Function GO:0070401 NADP+ binding
Molecular Function GO:0008948 oxaloacetate decarboxylase activity
Biological Process GO:0006108 malate metabolic process
Biological Process GO:0042866 pyruvate biosynthetic process

Reference

[1] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.