Search Results

Overview

Uniprot IDQ16822
Protein NamePhosphoenolpyruvate carboxykinase [GTP], mitochondrial
Gene NamePCK2
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
100 WLARTDPKDVARVES
108 DVARVESKTVIVTPS
261 GGNSLLGKKCFALRI
294 GITSPAGKKRYVAAA
410 SWLGKPWKPGDKEPC
414 KPWKPGDKEPCAHPN
457 IFGGRRPKGVPLVYE
489 STAAAEHKGKIIMHD
491 AAAEHKGKIIMHDPF
522 WLSMEGRKGAQLPRI
85 EQQGLIRKLPKYNNC
88 GLIRKLPKYNNCWLA

Function

Mitochondrial phosphoenolpyruvate carboxykinase that catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle (PubMed:28955899). Can play an active role in glyceroneogenesis and gluconeogenesis (PubMed:28955899). Also acts as a serine/threonine-protein kinase: phosphorylates and activates ACSL4, thereby promoting ferroptosis (PubMed:38720107)

Protein Sequence

10 MAALYRPGLR 20 LNWHGLSPLG 30 WPSCRSIQTL 40 RVLSGDLGQL 50 PTGIRDFVEH 60 SARLCQPEGI 70 HICDGTEAEN 80 TATLTLLEQQ 90 GLIRKLPKYN 100 NCWLARTDPK 110 DVARVESKTV 120 IVTPSQRDTV 130 PLPPGGARGQ 140 LGNWMSPADF 150 QRAVDERFPG 160 CMQGRTMYVL 170 PFSMGPVGSP 180 LSRIGVQLTD 190 SAYVVASMRI 200 MTRLGTPVLQ 210 ALGDGDFVKC 220 LHSVGQPLTG 230 QGEPVSQWPC 240 NPEKTLIGHV 250 PDQREIISFG 260 SGYGGNSLLG 270 KKCFALRIAS 280 RLARDEGWLA 290 EHMLILGITS 300 PAGKKRYVAA 310 AFPSACGKTN 320 LAMMRPALPG 330 WKVECVGDDI 340 AWMRFDSEGR 350 LRAINPENGF 360 FGVAPGTSAT 370 TNPNAMATIQ 380 SNTIFTNVAE 390 TSDGGVYWEG 400 IDQPLPPGVT 410 VTSWLGKPWK 420 PGDKEPCAHP 430 NSRFCAPARQ 440 CPIMDPAWEA 450 PEGVPIDAII 460 FGGRRPKGVP 470 LVYEAFNWRH 480 GVFVGSAMRS 490 ESTAAAEHKG 500 KIIMHDPFAM 510 RPFFGYNFGH 520 YLEHWLSMEG 530 RKGAQLPRIF 540 HVNWFRRDEA 550 GHFLWPGFGE 560 NARVLDWICR 570 RLEGEDSARE 580 TPIGLVPKEG 590 ALDLSGLRAI 600 DTTQLFSLPK 610 DFWEQEVRDI 620 RSYLTEQVNQ 630 DLPKEVLAEL 640 EALERRVHKM

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0005525 GTP binding
Molecular Function GO:0030145 manganese ion binding
Molecular Function GO:0004613 phosphoenolpyruvate carboxykinase (GTP) activity
Molecular Function GO:0004611 phosphoenolpyruvate carboxykinase activity
Molecular Function GO:0004674 protein serine/threonine kinase activity
Biological Process GO:0071549 cellular response to dexamethasone stimulus
Biological Process GO:0071333 cellular response to glucose stimulus
Biological Process GO:0032869 cellular response to insulin stimulus
Biological Process GO:0006094 gluconeogenesis
Biological Process GO:0046327 glycerol biosynthetic process from pyruvate
Biological Process GO:0070365 hepatocyte differentiation
Biological Process GO:0006107 oxaloacetate metabolic process
Biological Process GO:0160020 positive regulation of ferroptosis
Biological Process GO:0042594 response to starvation

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.