Search Results

Overview

Uniprot IDQ1JU68
Protein NameEukaryotic translation initiation factor 3 subunit A
Gene NameEif3a
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
100 YLKLAEEKTEAAKEE
1341 RESLRRTKNETDEDG
308 HLSREMRKNLTQDEM
632 LQEHEQIKKKTVRER
651 KKTELGAKAFKDIDI
901 DSEGTWRKGPEADSE

Function

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression

Protein Sequence

10 MPAYFQRPEN 20 ALKRANEFLE 30 VGKKQPALDV 40 LYDVMKSKKH 50 RTWQKIHEPI 60 MLKYLELCVD 70 LRKSHLAKEG 80 LYQYKNICQQ 90 VNIKSLEDVV 100 RAYLKLAEEK 110 TEAAKEESQQ 120 MVLDIEDLDN 130 IQTPESVLLS 140 AVSGEDTQDR 150 TDRLLLTPWV 160 KFLWESYRQC 170 LDLLRNNSRV 180 ERLYHDIAQQ 190 AFKFCLQYTR 200 KAEFRKLCDN 210 LRMHLSQIQR 220 HHNQSTAINL 230 NNPESQSMHL 240 ETRLVQLDSA 250 ISMELWQEAF 260 KAVEDIHGLF 270 SLSKKPPKPQ 280 LMANYYNKVS 290 TVFWKSGNAL 300 FHASTLHRLY 310 HLSREMRKNL 320 TQDEMQRMST 330 RVLLATLSIP 340 ITPERTDIAR 350 LLDMDGIIVE 360 KQRRLATLLG 370 LQAPPTRIGL 380 INDMVRFNVL 390 QYVVPEVKDL 400 YNWLEVEFNP 410 LKLCERVTKV 420 LNWVREQPEK 430 EPELQQYVPQ 440 LQNNTILRLL 450 QQVAQIYQSI 460 EFSRLTSLVP 470 FVDAFQLERA 480 IVDAARHCDL 490 QVRIDHTSRT 500 LSFGSDLNYA 510 TREDAPIGPH 520 LQSMPSEQIR 530 NQLTAMSSVL 540 AKALEVIKPA 550 HILQEKEEQH 560 QLAVTAYIKN 570 SRKEHQRILA 580 RRQTIEERKE 590 RLESLNIQRE 600 KEELEQREAE 610 LQKVRKAEEE 620 RLRQEAKERE 630 KERILQEHEQ 640 IKKKTVRERL 650 EQIKKTELGA 660 KAFKDIDIED 670 LEELDPDFIM 680 AKQVEQLEKE 690 KKELQERLKN 700 QEKKIDYFER 710 AKRLEEIPLI 720 KSAYEEQRVK 730 DMDLWEQQEE 740 ERITTMQLER 750 EKALEHKNRM 760 SRMLEDRDLF 770 VMRLKAARQS 780 VYEEKLKQFE 790 ERLAEERHNR 800 LEERKRQRKE 810 ERKITYYREK 820 EEEEQRRAEE 830 QMLKEREERE 840 RAERAKREEE 850 LREYQERVKK 860 LEEVERKKRQ 870 RELEIEERER 880 RREEERRLGE 890 DPLSRKDSRW 900 GDRDSEGTWR 910 KGPEADSEWR 920 RGPPEKEWRR 930 ETRDDERPHR 940 RDEDRLRRLG 950 GDDEERESSL 960 RPDDDRIPRR 970 GLDDDRGPRR 980 GPDEDRFSRR 990 GTDDDRPSWR 1000 NADDDRPPRR 1010 IGDDDRGSWR 1020 HTDDDRPPRR 1030 GLDDDRPPRR 1040 GLDDERGSWR 1050 TAEEDRGPRR 1060 GMDDDRGPRR 1070 GGADDERSSW 1080 RNADDDRGPR 1090 RGMDDDRGPR 1100 RGLDDDRGPW 1110 RNAAEDRISR 1120 RGADDDRGPW 1130 RNMDDDRVPR 1140 RGDDARPGPW 1150 RPFVKPGGWR 1160 EKEKAREESW 1170 GPPRESRPPE 1180 EREWDRDKEK 1190 DRDNQDREEN 1200 DKDLERDRDR 1210 ERDGDREDRF 1220 RRPRDEGGWR 1230 RGPAEESSSW 1240 RDSSRRDDRD 1250 RDDRRRDRDD 1260 RRDLRDLRDR 1270 RDLRDDRDRR 1280 GPPLRSEREE 1290 ASSWRRTDDR 1300 KDDRTEERDP 1310 PRRVPPPTLS 1320 RERERERDRE 1330 GEKEKASWRA 1340 EKDRESLRRT 1350 KNETDEDGWT TVRR

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0016282 eukaryotic 43S preinitiation complex
Cellular Component GO:0033290 eukaryotic 48S preinitiation complex
Cellular Component GO:0005852 eukaryotic translation initiation factor 3 complex
Cellular Component GO:0071540 eukaryotic translation initiation factor 3 complex, eIF3e
Cellular Component GO:0071541 eukaryotic translation initiation factor 3 complex, eIF3m
Cellular Component GO:0005874 microtubule
Cellular Component GO:0043614 multi-eIF complex
Cellular Component GO:0014069 postsynaptic density
Molecular Function GO:0003729 mRNA binding
Molecular Function GO:0030971 receptor tyrosine kinase binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0003743 translation initiation factor activity
Biological Process GO:0001732 formation of cytoplasmic translation initiation complex
Biological Process GO:0075522 IRES-dependent viral translational initiation
Biological Process GO:0070373 negative regulation of ERK1 and ERK2 cascade
Biological Process GO:0002188 translation reinitiation
Biological Process GO:0075525 viral translational termination-reinitiation

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.