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Overview

Uniprot IDQ1XH17
Protein NameTripartite motif-containing protein 72
Gene NameTrim72
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
232 VLEEVADKPQTEFLM
317 RVECSDQKAPPAGED
398 LEAHVEAKEPRALRT

Function

Muscle-specific E3 ubiquitin-protein ligase that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites (PubMed:19043407, PubMed:37770719). Its ubiquitination activity is mediated by E2 ubiquitin-conjugating enzymes UBE2D1, UBE2D2 and UBE2D3 (PubMed:37770719). Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site (PubMed:19043407, PubMed:19202355). This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation (PubMed:19043407). Probably acts upstream of the Ca(2+)-dependent membrane resealing process (PubMed:19043407). Required for transport of DYSF to sites of cell injury during repair patch formation (PubMed:19043407). Regulates membrane budding and exocytosis (PubMed:19029292). May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (PubMed:19202355)

Protein Sequence

10 MSAAPGLLRQ 20 ELSCPLCLQL 30 FDAPVTAECG 40 HSFCRACLIR 50 VAGEPAADGT 60 VACPCCQAPT 70 RPQALSTNLQ 80 LSRLVEGLAQ 90 VPQGHCEEHL 100 DPLSIYCEQD 110 RTLVCGVCAS 120 LGSHRGHRLL 130 PAAEAQARLK 140 TQLPQQKMQL 150 QEACMRKEKT 160 VAVLEHQLVE 170 VEETVRQFRG 180 AVGEQLGKMR 190 MFLAALESSL 200 DREAERVRGD 210 AGVALRRELS 220 SLNSYLEQLR 230 QMEKVLEEVA 240 DKPQTEFLMK 250 FCLVTSRLQK 260 ILSESPPPAR 270 LDIQLPVISD 280 DFKFQVWKKM 290 FRALMPALEE 300 LTFDPSSAHP 310 SLVVSSSGRR 320 VECSDQKAPP 330 AGEDTRQFDK 340 AVAVVAQQLL 350 SQGEHYWEVE 360 VGDKPRWALG 370 VMAADASRRG 380 RLHAVPSQGL 390 WLLGLRDGKI 400 LEAHVEAKEP 410 RALRTPERPP 420 ARIGLYLSFA 430 DGVLAFYDAS 440 NPDVLTPIFS 450 FHERLPGPVY 460 PIFDVCWHDK 470 GKNAQPLLLV GPEQEQA

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Molecular Function GO:0061630 ubiquitin protein ligase activity
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0006887 exocytosis
Biological Process GO:0045087 innate immune response
Biological Process GO:0007517 muscle organ development
Biological Process GO:0003012 muscle system process
Biological Process GO:0046627 negative regulation of insulin receptor signaling pathway
Biological Process GO:0043569 negative regulation of insulin-like growth factor receptor signaling pathway
Biological Process GO:0010832 negative regulation of myotube differentiation
Biological Process GO:0001778 plasma membrane repair
Biological Process GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
Biological Process GO:0051260 protein homooligomerization
Biological Process GO:0006900 vesicle budding from membrane
Cellular Component GO:0030659 cytoplasmic vesicle membrane
Cellular Component GO:0042383 sarcolemma
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0031435 mitogen-activated protein kinase kinase kinase binding
Molecular Function GO:0001786 phosphatidylserine binding
Molecular Function GO:0031624 ubiquitin conjugating enzyme binding

Reference

[1] Chang J, Wu W, Qian P, Lu Z, He X et al.. Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue.. Front Cell Dev Biol 12:1472338. 2024. PMID: 39935788.

[2] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[3] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.