Search Results

Overview

Uniprot IDQ27HV0
Protein NameUDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit
Gene NameOGT
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
16 ADSTEPTKRMLSFQG
908 IFSPVAPKEEHVRRG

Function

Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, AMPK, ATG4B, CAPRIN1, EZH2, FNIP1, GSDMD, KRT7, LMNA, LMNB1, LMNB2, RPTOR, HOXA1, PFKL, KMT2E/MLL5, MAPT/TAU, TET2, RBL2, RET, NOD2 and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing (By similarity). Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling (By similarity). Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity. Plays a key role in chromatin structure by mediating O-GlcNAcylation of 'Ser-112' of histone H2B: recruited to CpG-rich transcription start sites of active genes via its interaction with TET proteins (TET1, TET2 or TET3). As part of the NSL complex indirectly involved in acetylation of nucleosomal histone H4 on several lysine residues. O-GlcNAcylation of 'Ser-75' of EZH2 increases its stability, and facilitating the formation of H3K27me3 by the PRC2/EED-EZH2 complex. Stabilizes KMT2E/MLL5 by mediating its glycosylation, thereby preventing KMT2E/MLL5 ubiquitination (By similarity). Regulates circadian oscillation of the clock genes and glucose homeostasis in the liver (By similarity). Stabilizes clock proteins BMAL1 and CLOCK through O-glycosylation, which prevents their ubiquitination and subsequent degradation (By similarity). Promotes the CLOCK-BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2 and CRY1/2. O-glycosylates HCFC1 and regulates its proteolytic processing and transcriptional activity. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1 (By similarity). Regulates mitochondrial motility in neurons by mediating glycosylation of TRAK1 (By similarity). Promotes autophagy by mediating O-glycosylation of ATG4B. Acts as a regulator of mTORC1 signaling by mediating O-glycosylation of RPTOR and FNIP1: O-GlcNAcylation of RPTOR in response to glucose sufficiency promotes activation of the mTORC1 complex (By similarity)

Protein Sequence

10 MASSVGNVAD 20 STEPTKRMLS 30 FQGLAELAHR 40 EYQAGDFEAA 50 ERHCMQLWRQ 60 EPDNTGVLLL 70 LSSIHFQCRR 80 LDRSAHFSTL 90 AIKQNPLLAE 100 AYSNLGNVYK 110 ERGQLQEAIE 120 HYRHALRLKP 130 DFIDGYINLA 140 AALVAAGDME 150 GAVQAYVSAL 160 QYNPDLYCVR 170 SDLGNLLKAL 180 GRLEEAKACY 190 LKAIETQPNF 200 AVAWSNLGCV 210 FNAQGEIWLA 220 IHHFEKAVTL 230 DPNFLDAYIN 240 LGNVLKEARI 250 FDRAVAAYLR 260 ALSLSPNHAV 270 VHGNLACVYY 280 EQGLIDLAID 290 TYRRAIELQP 300 HFPDAYCNLA 310 NALKEKGSVA 320 EAEDCYNTAL 330 RLCPTHADSL 340 NNLANIKREQ 350 GNIEEAVRLY 360 RKALEVFPEF 370 AAAHSNLASV 380 LQQQGKLQEA 390 LMHYKEAIRI 400 SPTFADAYSN 410 MGNTLKEMQD 420 VQGALQCYTR 430 AIQINPAFAD 440 AHSNLASIHK 450 DSGNIPEAIA 460 SYRTALKLKP 470 DFPDAYCNLA 480 HCLQIVCDWT 490 DYDERMKKLV 500 SIVADQLEKN 510 RLPSVHPHHS 520 MLYPLSHGFR 530 KAIAERHGNL 540 CLDKINVLHK 550 PPYEHPKDLK 560 LSDGRLRVGY 570 VSSDFGNHPT 580 SHLMQSIPGM 590 HNPDKFEVFC 600 YALSPDDGTN 610 FRVKVMAEAN 620 HFIDLSQIPC 630 NGKAADRIHQ 640 DGIHILVNMN 650 GYTKGARNEL 660 FALRPAPIQA 670 MWLGYPGTSG 680 ALFMDYIITD 690 QETSPAEVAE 700 QYSEKLAYMP 710 HTFFIGDHAN 720 MFPHLKKKAV 730 IDFKSNGHIY 740 DNRIVLNGID 750 LKAFLDSLPD 760 VKIVKMKCPD 770 GGDNADSSNT 780 ALNMPVIPMN 790 TIAEAVIEMI 800 NRGQIQITIN 810 GFSISNGLAT 820 TQINNKAATG 830 EEVPRTIIVT 840 TRSQYGLPED 850 AIVYCNFNQL 860 YKIDPSTLQM 870 WANILKRVPN 880 SVLWLLRFPA 890 VGEPNIQQYA 900 QNMGLPQNRI 910 IFSPVAPKEE 920 HVRRGQLADV 930 CLDTPLCNGH 940 TTGMDVLWAG 950 TPMVTMPGET 960 LASRVAASQL 970 TCLGCLELIA 980 KNRQEFEDIA 990 VKLGTDLEYL 1000 KKIRGKVWKQ 1010 RISSPLFNTK 1020 QYTMELERLY 1030 LQMWEHYAAG 1040 NKPDHMIKPV EVTESA

Gene Ontology

Classification GO ID Description
Molecular Function GO:0016757 glycosyltransferase activity
Cellular Component GO:0005829 cytosol
Cellular Component GO:0000123 histone acetyltransferase complex
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005886 plasma membrane
Molecular Function GO:0005547 phosphatidylinositol-3,4,5-trisphosphate binding
Molecular Function GO:0097363 protein O-acetylglucosaminyltransferase activity
Biological Process GO:0006915 apoptotic process
Biological Process GO:0071333 cellular response to glucose stimulus
Biological Process GO:0006325 chromatin organization
Biological Process GO:0032922 circadian regulation of gene expression
Biological Process GO:0160076 negative regulation of non-canonical inflammasome complex assembly
Biological Process GO:0031397 negative regulation of protein ubiquitination
Biological Process GO:0045862 positive regulation of proteolysis
Biological Process GO:1904263 positive regulation of TORC1 signaling
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0006493 protein O-linked glycosylation
Biological Process GO:0016485 protein processing
Biological Process GO:0006111 regulation of gluconeogenesis
Biological Process GO:0006110 regulation of glycolytic process

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.