Overview
| Uniprot ID | Q28944 |
|---|
| Protein Name | Procathepsin L |
|---|
| Gene Name | CTSL |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 123 |
KSVDWREKGYVTAVK |
Function
Thiol protease important for the overall degradation of proteins in lysosomes (By similarity). Plays a critical for normal cellular functions such as general protein turnover, antigen processing and bone remodeling. Involved in the solubilization of cross-linked TG/thyroglobulin and in the subsequent release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen (By similarity). In neuroendocrine chromaffin cells secretory vesicles, catalyzes the prohormone proenkephalin processing to the active enkephalin peptide neurotransmitter (By similarity). In thymus, regulates CD4(+) T cell positive selection by generating the major histocompatibility complex class II (MHCII) bound peptide ligands presented by cortical thymic epithelial cells. Also mediates invariant chain processing in cortical thymic epithelial cells. Major elastin-degrading enzyme at neutral pH. Accumulates as a mature and active enzyme in the extracellular space of antigen presenting cells (APCs) to regulate degradation of the extracellular matrix in the course of inflammation (By similarity). Secreted form generates endostatin from COL18A1 (By similarity). Critical for cardiac morphology and function. Plays an important role in hair follicle morphogenesis and cycling, as well as epidermal differentiation (By similarity). Required for maximal stimulation of steroidogenesis by TIMP1 (By similarity)
Protein Sequence
10
MKPSLFLTAL
20
CLGIASAAPK
30
LDQNLDADWY
40
KWKATHGRLY
50
GMNEEGWRRA
60
VWEKNMKMIE
70
LHNQEYSQGK
80
HGFSMAMNAF
90
GDMTNEEFRQ
100
VMNGFQNQKH
110
KKGKVFHESL
120
VLEVPKSVDW
130
REKGYVTAVK
140
NQGQCGSCWA
150
FSATGALEGQ
160
MFRKTGKLVS
170
LSEQNLVDCS
180
RPQGNQGCNG
190
GLMDNAFQYV
200
KDNGGLDTEE
210
SYPYLGRETN
220
SCTYKPECSA
230
ANDTGFVDIP
240
QREKALMKAV
250
ATVGPISVAI
260
DAGHSSFQFY
270
KSGIYYDPDC
280
SSKDLDHGVL
290
VVGYGFEGTD
300
SNSSKFWIVK
310
NSWGPEWGWN
320
GYVKMAKDQN
330
NHCGISTAAS
YPTV
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0016324 |
apical plasma membrane |
| Cellular Component |
GO:0042583 |
chromaffin granule |
| Cellular Component |
GO:0005615 |
extracellular space |
| Cellular Component |
GO:0005764 |
lysosome |
| Molecular Function |
GO:0004197 |
cysteine-type endopeptidase activity |
| Molecular Function |
GO:0004175 |
endopeptidase activity |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Biological Process |
GO:0048002 |
antigen processing and presentation of peptide antigen |
| Biological Process |
GO:0043373 |
CD4-positive, alpha-beta T cell lineage commitment |
| Biological Process |
GO:0030574 |
collagen catabolic process |
| Biological Process |
GO:0060309 |
elastin catabolic process |
| Biological Process |
GO:0034230 |
enkephalin processing |
| Biological Process |
GO:0016540 |
protein autoprocessing |
| Biological Process |
GO:0051603 |
proteolysis involved in protein catabolic process |
| Biological Process |
GO:0031638 |
zymogen activation |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.