Overview
| Uniprot ID | Q29099 |
|---|
| Protein Name | Polypyrimidine tract-binding protein 1 |
|---|
| Gene Name | PTBP1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 134 |
YIQFSNHKELKTDSS |
| 137 |
FSNHKELKTDSSPNQ |
| 259 |
TLRIDFSKLTSLNVK |
| 266 |
KLTSLNVKYNNDKSR |
| 394 |
YGDVQRVKILFNKKE |
| 436 |
PVRITLSKHQNVQLP |
| 454 |
QEDQGLTKDYGNSPL |
| 511 |
GGIVKGFKFFQKDRK |
Function
Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10. Binds to polypyrimidine-rich controlling element (PCE) of CFTR and promotes exon skipping of CFTR exon 9, thereby antagonizing TIA1 and its role in exon inclusion of CFTR exon 9. Plays a role in the splicing of pyruvate kinase PKM by binding repressively to a polypyrimidine tract flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform
Protein Sequence
10
MDGIVPDIAV
20
GTKRGSDELF
30
SACVTNGPFI
40
MSSNSASTAN
50
GNDSKKFKGD
60
NRSAGVPSRV
70
IHIRKLPSDV
80
TEGEVISLGL
90
PFGKVTNLLM
100
LKGKNQAFIE
110
MNTEEAANTM
120
VNYYTSVTPV
130
LRGQPIYIQF
140
SNHKELKTDS
150
SPNQARAQAA
160
LQAVNSVQSG
170
NLALAASAAA
180
VDAGMAMAGQ
190
SPVLRIIVEN
200
LFYPVTLDVL
210
HQIFSKFGTV
220
LKIITFTKNN
230
QFQALLQYAD
240
PVSAQHAKLS
250
LDGQNIYNAC
260
CTLRIDFSKL
270
TSLNVKYNND
280
KSRDYTRPDL
290
PSGDNQPSLD
300
QTMAAAFGAP
310
GIMSASPYAG
320
AGFPPTFAIP
330
QAAGLSVPNV
340
HGALAPLAIP
350
SAAAAAAAAG
360
RIAIPGLAGA
370
GNSVLLVSNL
380
NPERVTPQSL
390
FILFGVYGDV
400
QRVKILFNKK
410
ENALVQMADG
420
SQAQLAMSHL
430
NGHKLHGKPV
440
RITLSKHQNV
450
QLPREGQEDQ
460
GLTKDYGNSP
470
LHRFKKPGSK
480
NFQNIFPPSA
490
TLHLSNIPPS
500
ISEEDLKILF
510
SSNGGIVKGF
520
KFFQKDRKMA
530
LIQMGSVEEA
540
IQALIDLHNH
550
DLGENHHLRV
SFSKSTI
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0003729 |
mRNA binding |
| Biological Process |
GO:0006397 |
mRNA processing |
| Biological Process |
GO:0048025 |
negative regulation of mRNA splicing, via spliceosome |
| Biological Process |
GO:0051148 |
negative regulation of muscle cell differentiation |
| Biological Process |
GO:0000381 |
regulation of alternative mRNA splicing, via spliceosome |
| Biological Process |
GO:0045595 |
regulation of cell differentiation |
| Biological Process |
GO:0043484 |
regulation of RNA splicing |
| Biological Process |
GO:0008380 |
RNA splicing |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.