Overview
| Uniprot ID | Q29307 |
|---|
| Protein Name | ATPase inhibitor, mitochondrial |
|---|
| Gene Name | ATP5IF1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 49 |
DAGGAFGKKEQADEE |
| 50 |
AGGAFGKKEQADEER |
| 71 |
REQLAALKKHHENEI |
| 83 |
NEISHHVKEIERLQK |
| 90 |
KEIERLQKEIERHKQ |
Function
Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase (By similarity). Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme (By similarity)
Protein Sequence
10
MAATALAVRS
20
RIGAWSVWAM
30
QSRGFSSDTP
40
EGVRSGAGAV
50
RDAGGAFGKK
60
EQADEERYFR
70
ARAREQLAAL
80
KKHHENEISH
90
HVKEIERLQK
100
EIERHKQSIK
KLKNDDDD
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0009986 |
cell surface |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Cellular Component |
GO:0032991 |
protein-containing complex |
| Molecular Function |
GO:0043532 |
angiostatin binding |
| Molecular Function |
GO:0051117 |
ATPase binding |
| Molecular Function |
GO:0042030 |
ATPase inhibitor activity |
| Molecular Function |
GO:0005516 |
calmodulin binding |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0140260 |
mitochondrial proton-transporting ATP synthase complex binding |
| Biological Process |
GO:0030218 |
erythrocyte differentiation |
| Biological Process |
GO:0006783 |
heme biosynthetic process |
| Biological Process |
GO:0051882 |
mitochondrial depolarization |
| Biological Process |
GO:0010667 |
negative regulation of cardiac muscle cell apoptotic process |
| Biological Process |
GO:0001937 |
negative regulation of endothelial cell proliferation |
| Biological Process |
GO:0051346 |
negative regulation of hydrolase activity |
| Biological Process |
GO:1905707 |
negative regulation of mitochondrial ATP synthesis coupled proton transport |
| Biological Process |
GO:1903052 |
positive regulation of proteolysis involved in protein catabolic process |
| Biological Process |
GO:1905091 |
positive regulation of type 2 mitophagy |
| Biological Process |
GO:1903214 |
regulation of protein targeting to mitochondrion |
| Biological Process |
GO:0002931 |
response to ischemia |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.