Overview
| Uniprot ID | Q2TAA5 |
|---|
| Protein Name | GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase |
|---|
| Gene Name | ALG11 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 12 |
ERSWCLCKLLRFFYS |
Function
GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes, on the cytoplasmic face of the endoplasmic reticulum, the addition of the fourth and fifth mannose residues to the dolichol-linked oligosaccharide chain, to produce Man(5)GlcNAc(2)-PP-dolichol core oligosaccharide (PubMed:20080937). Man(5)GlcNAc(2)-PP-dolichol is a substrate for ALG3, the following enzyme in the biosynthetic pathway (PubMed:10581255)
Protein Sequence
10
MAAGERSWCL
20
CKLLRFFYSL
30
FFPGLIVCGT
40
LCVCLVIVLW
50
GIRLLLQRKK
60
KLVSTSKNGK
70
NQMVIAFFHP
80
YCNAGGGGER
90
VLWCALRALQ
100
KKYPEAVYVV
110
YTGDVNVNGQ
120
QILEGAFRRF
130
NIRLIHPVQF
140
VFLRKRYLVE
150
DSLYPHFTLL
160
GQSLGSIFLG
170
WEALMQCVPD
180
VYIDSMGYAF
190
TLPLFKYIGG
200
CQVGSYVHYP
210
TISTDMLSVV
220
KNQNIGFNNA
230
AFITRNPFLS
240
KVKLIYYYLF
250
AFIYGLVGSC
260
SDVVMVNSSW
270
TLNHILSLWK
280
VGNCTNIVYP
290
PCDVQTFLDI
300
PLHEKKMTPG
310
HLLVSVGQFR
320
PEKNHPLQIR
330
AFAKLLNKKM
340
VESPPSLKLV
350
LIGGCRNKDD
360
ELRVNQLRRL
370
SEDLGVQEYV
380
EFKINIPFDE
390
LKNYLSEATI
400
GLHTMWNEHF
410
GIGVVECMAA
420
GTIILAHNSG
430
GPKLDIVVPH
440
EGDITGFLAE
450
SEEDYAETIA
460
HILSMSAEKR
470
LQIRKSARAS
480
VSRFSDQEFE
490
VTFLSSVEKL
FK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005789 |
endoplasmic reticulum membrane |
| Cellular Component |
GO:0016020 |
membrane |
| Molecular Function |
GO:0000026 |
alpha-1,2-mannosyltransferase activity |
| Molecular Function |
GO:0004377 |
GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase activity |
| Biological Process |
GO:0006488 |
dolichol-linked oligosaccharide biosynthetic process |
| Biological Process |
GO:0006487 |
protein N-linked glycosylation |
Reference
[1] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.