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Overview

Uniprot IDQ3B8Q2
Protein NameEukaryotic initiation factor 4A-III
Gene NameEif4a3
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
19 SARKRLLKEEDMTKV
289 IFCNTKRKVDWLTEK

Function

ATP-dependent RNA helicase. Involved in pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms; the function is different from the established EJC assembly. Involved in craniofacial development

Protein Sequence

10 MAATATMATS 20 GSARKRLLKE 30 EDMTKVEFET 40 SEEVDVTPTF 50 DTMGLREDLL 60 RGIYAYGFEK 70 PSAIQQRAIK 80 QIIKGRDVIA 90 QSQSGTGKTA 100 TFSISVLQCL 110 DIQVRETQAL 120 ILAPTRELAV 130 QIQKGLLALG 140 DYMNVQCHAC 150 IGGTNVGEDI 160 RKLDYGQHVV 170 AGTPGRVFDM 180 IRRRSLRTRA 190 IKMLVLDEAD 200 EMLNKGFKEQ 210 IYDVYRYLPP 220 ATQVVLISAT 230 LPHEILEMTN 240 KFMTDPIRIL 250 VKRDELTLEG 260 IKQFFVAVER 270 EEWKFDTLCD 280 LYDTLTITQA 290 VIFCNTKRKV 300 DWLTEKMREA 310 NFTVSSMHGD 320 MPQKERESIM 330 KEFRSGASRV 340 LISTDVWARG 350 LDVPQVSLII 360 NYDLPNNREL 370 YIHRIGRSGR 380 YGRKGVAINF 390 VKNDDIRILR 400 DIEQYYSTQI 410 DEMPMNVADL I

Gene Ontology

Classification GO ID Description
Biological Process GO:0006364 rRNA processing
Cellular Component GO:0071013 catalytic step 2 spliceosome
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0030425 dendrite
Cellular Component GO:0035145 exon-exon junction complex
Cellular Component GO:0098978 glutamatergic synapse
Cellular Component GO:0043025 neuronal cell body
Cellular Component GO:0016607 nuclear speck
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0071020 post-spliceosomal complex
Cellular Component GO:0098794 postsynapse
Cellular Component GO:1990904 ribonucleoprotein complex
Cellular Component GO:0005681 spliceosomal complex
Cellular Component GO:0071006 U2-type catalytic step 1 spliceosome
Cellular Component GO:0071007 U2-type catalytic step 2 spliceosome
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0003729 mRNA binding
Molecular Function GO:0008143 poly(A) binding
Molecular Function GO:0043021 ribonucleoprotein complex binding
Molecular Function GO:0003724 RNA helicase activity
Molecular Function GO:0035613 RNA stem-loop binding
Molecular Function GO:0035368 selenocysteine insertion sequence binding
Biological Process GO:0008306 associative learning
Biological Process GO:1990416 cellular response to brain-derived neurotrophic factor stimulus
Biological Process GO:0072715 cellular response to selenite ion
Biological Process GO:0048701 embryonic cranial skeleton morphogenesis
Biological Process GO:0035640 exploration behavior
Biological Process GO:0006406 mRNA export from nucleus
Biological Process GO:0016071 mRNA metabolic process
Biological Process GO:0000398 mRNA splicing, via spliceosome
Biological Process GO:0090394 negative regulation of excitatory postsynaptic potential
Biological Process GO:0010629 negative regulation of gene expression
Biological Process GO:1904570 negative regulation of selenocysteine incorporation
Biological Process GO:0017148 negative regulation of translation
Biological Process GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
Biological Process GO:0048026 positive regulation of mRNA splicing, via spliceosome
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0045727 positive regulation of translation
Biological Process GO:0000381 regulation of alternative mRNA splicing, via spliceosome
Biological Process GO:2000622 regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
Biological Process GO:0099578 regulation of translation at postsynapse, modulating synaptic transmission

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.