Overview
| Uniprot ID | Q3T1J1 |
|---|
| Protein Name | Eukaryotic translation initiation factor 5A-1 |
|---|
| Gene Name | Eif5a |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 121 |
LPEGDLGKEIEQKYD |
| 39 |
VLKGRPCKIVEMSTS |
| 67 |
GIDIFTGKKYEDICP |
Function
Translation factor that promotes translation elongation and termination, particularly upon ribosome stalling at specific amino acid sequence contexts (By similarity). Binds between the exit (E) and peptidyl (P) site of the ribosome and promotes rescue of stalled ribosome: specifically required for efficient translation of polyproline-containing peptides as well as other motifs that stall the ribosome. Acts as a ribosome quality control (RQC) cofactor by joining the RQC complex to facilitate peptidyl transfer during CAT tailing step (By similarity). Also involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Also regulates TNF-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival (By similarity). May play an important role in brain development and function, and in skeletal muscle stem cell differentiation (PubMed:18606156, PubMed:19006180). Is required for autophagy by assisting the ribosome in translating the ATG3 protein at a specific amino acid sequence, the 'ASP-ASP-Gly' motif, leading to the increase of the efficiency of ATG3 translation and facilitation of LC3B lipidation and autophagosome formation (By similarity)
Protein Sequence
10
MADDLDFETG
20
DAGASATFPM
30
QCSALRKNGF
40
VVLKGRPCKI
50
VEMSTSKTGK
60
HGHAKVHLVG
70
IDIFTGKKYE
80
DICPSTHNMD
90
VPNIKRNDFQ
100
LIGIQDGYLS
110
LLQDSGEVRE
120
DLRLPEGDLG
130
KEIEQKYDCG
140
EEILITVLSA
150
MTEEAAVAIK
AMAK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005642 |
annulate lamellae |
| Biological Process |
GO:1902255 |
positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator |
| Biological Process |
GO:0051149 |
positive regulation of muscle cell differentiation |
| Biological Process |
GO:2000379 |
positive regulation of reactive oxygen species metabolic process |
| Biological Process |
GO:0045944 |
positive regulation of transcription by RNA polymerase II |
| Biological Process |
GO:0006414 |
translational elongation |
| Biological Process |
GO:0033209 |
tumor necrosis factor-mediated signaling pathway |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0030425 |
dendrite |
| Cellular Component |
GO:0005789 |
endoplasmic reticulum membrane |
| Cellular Component |
GO:0043025 |
neuronal cell body |
| Cellular Component |
GO:0005643 |
nuclear pore |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0045202 |
synapse |
| Molecular Function |
GO:0043022 |
ribosome binding |
| Molecular Function |
GO:0003723 |
RNA binding |
| Molecular Function |
GO:0003746 |
translation elongation factor activity |
| Molecular Function |
GO:0017070 |
U6 snRNA binding |
| Biological Process |
GO:0097067 |
cellular response to thyroid hormone stimulus |
| Biological Process |
GO:0098586 |
cellular response to virus |
| Biological Process |
GO:0043066 |
negative regulation of apoptotic process |
| Biological Process |
GO:0043065 |
positive regulation of apoptotic process |
| Biological Process |
GO:0010666 |
positive regulation of cardiac muscle cell apoptotic process |
| Biological Process |
GO:0007204 |
positive regulation of cytosolic calcium ion concentration |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.