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Overview

Uniprot IDQ3UUI3
Protein NameAcyl-coenzyme A thioesterase THEM4
Gene NameThem4
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
98 KKEEQMSKAQQFTRS

Function

Has acyl-CoA thioesterase activity towards medium and long-chain (C14 to C18) fatty acyl-CoA substrates, and probably plays a role in mitochondrial fatty acid metabolism (By similarity). Plays a role in the apoptotic process, possibly via its regulation of AKT1 activity (PubMed:19421406)

Protein Sequence

10 MLRNCAMRLR 20 TLGATPARRP 30 GAARRLFSSE 40 KVIRKDYALP 50 NPSWTKDLRL 60 LFDQFMKKCE 70 DGSWKRMPSH 80 RQNPTRAIQE 90 FQTLFVDSKF 100 KKEEQMSKAQ 110 QFTRSFEEGL 120 GFEYAMFYNK 130 VEKRTVSLFQ 140 GGLHLQGVPG 150 FVHGGAIATI 160 IDVTTGTCAI 170 SEGVAMTANL 180 NITYKKPIPL 190 LSVVVVNSQL 200 QKIEGRKLFV 210 SCTIQSIDEK 220 TLYTEATALF 230 IKLDPEKPLT

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005743 mitochondrial inner membrane
Cellular Component GO:0005758 mitochondrial intermembrane space
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0032587 ruffle membrane
Molecular Function GO:0052816 long-chain fatty acyl-CoA hydrolase activity
Biological Process GO:0006631 fatty acid metabolic process
Biological Process GO:0051898 negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
Biological Process GO:1902108 regulation of mitochondrial membrane permeability involved in apoptotic process

Reference

[1] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.