Search Results

Overview

Uniprot ID	Q4G061
Protein Name	Eukaryotic translation initiation factor 3 subunit B
Gene Name	Eif3b
Organism	Rattus norvegicus

Kla Sites from experimental identification

Position	Flanking peptide
240	ADGYKLDKQHTFRVN

Function

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression

Protein Sequence

10 MQDAENVAAP 20 EAAEERAEPA 30 RQQPVSESPP 40 TDEAAGSGGS 50 EVGRTEDAEE 60 DAEARPEPEV 70 RAKPAAQSEE 80 ETAASPAASP 90 TPQSAQEPSA 100 PGKAEAGGEQ 110 ARHPSARAEE 120 EGGSDGSAAE 130 AEPRALENGE 140 ADEPSFSDPE 150 DFVDDVSEEE 160 LLGDVLKDRP 170 QEADGIDSVI 180 VVDNVPQVGP 190 DRLEKLKNVI 200 HKIFSKFGKI 210 INDYYPEEDG 220 KTKGYIFLEY 230 ASPAHAVDAV 240 KNADGYKLDK 250 QHTFRVNLFT 260 DFDKYMTISD 270 EWDIPEKQPF 280 KDLGNLRYWL 290 EEAECRDQYS 300 VIFESGDRTS 310 IFWNDVKDPV 320 SIEERARWTE 330 TYVRWSPKGT 340 YLATFHQRGI 350 ALWGGDKFKQ 360 IQRFSHQGVQ 370 LIDFSPCERY 380 LVTFSPLMDT 390 QDDPQAIIIW 400 DILTGHKKRG 410 FHCESSAHWP 420 IFKWSHDGKF 430 FARMTLDTLS 440 IYETPSMGLL 450 DKKSLKISGI 460 KDFSWSPGGN 470 IIAFWVPEDK 480 DIPARVTLMQ 490 LPTRQEIRVR 500 NLFNVVDCKL 510 HWQKNGDYLC 520 VKVDRTPKGT 530 QGVVTNFEIF 540 RMREKQVPVD 550 VVEMKETIIA 560 FAWEPNGSKF 570 AVLHGEAPRI 580 SVSFYHVKSN 590 GKIELIKMFD 600 KQQANTIFWS 610 PQGQFVVLAG 620 LRSMNGALAF 630 VDTSDCTVMN 640 IAEHYMASDV 650 EWDPTGRYVV 660 TSVSWWSHKV 670 DNAYWLWTFQ 680 GRLLQKNNKD 690 RFCQLLWRPR 700 PPTLLSQDQI 710 KQIKKDLKKY 720 SKIFEQKDRL 730 SQSKASKELV 740 ERRRTMMEDF 750 RQYRKMAQEL 760 YMKQKNERLE 770 LRGGVDTDEL 780 DSNVDDWEEE 790 TIEFFVTEEV IPLGSQE

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0010494	cytoplasmic stress granule
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0016282	eukaryotic 43S preinitiation complex
Cellular Component	GO:0033290	eukaryotic 48S preinitiation complex
Cellular Component	GO:0005852	eukaryotic translation initiation factor 3 complex
Cellular Component	GO:0071541	eukaryotic translation initiation factor 3 complex, eIF3m
Cellular Component	GO:0045202	synapse
Molecular Function	GO:0003723	RNA binding
Molecular Function	GO:0003743	translation initiation factor activity
Molecular Function	GO:0031369	translation initiation factor binding
Biological Process	GO:0001732	formation of cytoplasmic translation initiation complex
Biological Process	GO:0075522	IRES-dependent viral translational initiation
Biological Process	GO:0006446	regulation of translational initiation
Biological Process	GO:0006413	translational initiation
Biological Process	GO:0075525	viral translational termination-reinitiation

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.