Search Results

Overview

Uniprot ID	Q4G176
Protein Name	Malonate--CoA ligase ACSF3, mitochondrial
Gene Name	ACSF3
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
478	RTSVDIIKTGGYKVS
563	IPRNQMGKIDKKALI

Function

Catalyzes the initial reaction in intramitochondrial fatty acid synthesis, by activating malonate and methylmalonate, but not acetate, into their respective CoA thioester (PubMed:21841779, PubMed:21846720). May have some preference toward very-long-chain substrates (PubMed:17762044)

Protein Sequence

10 MLPHVVLTFR 20 RLGCALASCR 30 LAPARHRGSG 40 LLHTAPVARS 50 DRSAPVFTRA 60 LAFGDRIALV 70 DQHGRHTYRE 80 LYSRSLRLSQ 90 EICRLCGCVG 100 GDLREERVSF 110 LCANDASYVV 120 AQWASWMSGG 130 VAVPLYRKHP 140 AAQLEYVICD 150 SQSSVVLASQ 160 EYLELLSPVV 170 RKLGVPLLPL 180 TPAIYTGAVE 190 EPAEVPVPEQ 200 GWRNKGAMII 210 YTSGTTGRPK 220 GVLSTHQNIR 230 AVVTGLVHKW 240 AWTKDDVILH 250 VLPLHHVHGV 260 VNALLCPLWV 270 GATCVMMPEF 280 SPQQVWEKFL 290 SSETPRINVF 300 MAVPTIYTKL 310 MEYYDRHFTQ 320 PHAQDFLRAV 330 CEEKIRLMVS 340 GSAALPLPVL 350 EKWKNITGHT 360 LLERYGMTEI 370 GMALSGPLTT 380 AVRLPGSVGT 390 PLPGVQVRIV 400 SENPQREACS 410 YTIHAEGDER 420 GTKVTPGFEE 430 KEGELLVRGP 440 SVFREYWNKP 450 EETKSAFTLD 460 GWFKTGDTVV 470 FKDGQYWIRG 480 RTSVDIIKTG 490 GYKVSALEVE 500 WHLLAHPSIT 510 DVAVIGVPDM 520 TWGQRVTAVV 530 TLREGHSLSH 540 RELKEWARNV 550 LAPYAVPSEL 560 VLVEEIPRNQ 570 MGKIDKKALI RHFHPS

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005759	mitochondrial matrix
Cellular Component	GO:0005739	mitochondrion
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0016405	CoA-ligase activity
Molecular Function	GO:0090409	malonyl-CoA synthetase activity
Molecular Function	GO:0031957	very long-chain fatty acid-CoA ligase activity
Biological Process	GO:0006633	fatty acid biosynthetic process
Biological Process	GO:0006631	fatty acid metabolic process
Biological Process	GO:0035338	long-chain fatty-acyl-CoA biosynthetic process
Biological Process	GO:0090410	malonate catabolic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[3] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[4] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[5] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.