Overview
| Uniprot ID | Q4V886 |
|---|
| Protein Name | RNA polymerase II-associated factor 1 homolog |
|---|
| Gene Name | Paf1 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 119 |
IQAPTSSKRSQQHAK |
| 62 |
QNRFVQYKATSLEKQ |
Function
Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Connects PAF1C with the RNF20/40 E3 ubiquitin-protein ligase complex. Involved in polyadenylation of mRNA precursors (By similarity)
Protein Sequence
10
MAPTIQTQAQ
20
REDGHRPNSH
30
RTLPERSGVV
40
CRVKYCNSLP
50
DIPFDPKFIT
60
YPFDQNRFVQ
70
YKATSLEKQH
80
KHDLLTEPDL
90
GVTIDLINPD
100
TYRIDPNVLL
110
DPADEKLLEE
120
EIQAPTSSKR
130
SQQHAKVVPW
140
MRKTEYISTE
150
FNRYGISNEK
160
PEVKIGVSVK
170
QQFTEEEIYK
180
DRDSQITAIE
190
KTFEDAQKSI
200
SQHYSKPRVT
210
PVEVMPVFPD
220
FKMWINPCAQ
230
VIFDSDPAPK
240
DTSGAAALEM
250
MSQAMIRGMM
260
DEEGNQFVAY
270
FLPVEETLKK
280
RKRDQEEEMD
290
YAPDDVYDYK
300
IAREYNWNVK
310
NKASKGYEEN
320
YFFIFREGDG
330
VYYNELETRV
340
RLSKRRAKAG
350
VQSGTNALLV
360
VKHRDMNEKE
370
LEAQEARKAQ
380
LENHEPEEEE
390
EEEMEAEEKE
400
AGGSDEEHEK
410
GSSSEKEGSE
420
DERSGSESDR
430
EEGDRDEASD
440
KSGSGEDESS
450
EDEARAARDK
460
EEIFGSDADS
470
EDDADSDDED
480
RGQAHRGSDN
490
DSDSGSDGGG
500
QRSRSQSRSR
510
SRSASPFPSG
520
SEHSAQEDGS
530
EAAASDSSEA
DSDSD
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0016593 |
Cdc73/Paf1 complex |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0001650 |
fibrillar center |
| Cellular Component |
GO:0016020 |
membrane |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0003682 |
chromatin binding |
| Molecular Function |
GO:0000993 |
RNA polymerase II complex binding |
| Biological Process |
GO:0071222 |
cellular response to lipopolysaccharide |
| Biological Process |
GO:0001711 |
endodermal cell fate commitment |
| Biological Process |
GO:0010467 |
gene expression |
| Biological Process |
GO:0031124 |
mRNA 3'-end processing |
| Biological Process |
GO:0045638 |
negative regulation of myeloid cell differentiation |
| Biological Process |
GO:0000122 |
negative regulation of transcription by RNA polymerase II |
| Biological Process |
GO:1902808 |
positive regulation of cell cycle G1/S phase transition |
| Biological Process |
GO:0045944 |
positive regulation of transcription by RNA polymerase II |
| Biological Process |
GO:0034504 |
protein localization to nucleus |
| Biological Process |
GO:0019827 |
stem cell population maintenance |
| Biological Process |
GO:0006368 |
transcription elongation by RNA polymerase II |
| Biological Process |
GO:0016055 |
Wnt signaling pathway |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.