Overview
| Uniprot ID | Q569Z6 |
|---|
| Protein Name | Thyroid hormone receptor-associated protein 3 |
|---|
| Gene Name | Thrap3 |
|---|
| Organism | Mus musculus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 452 |
DEPKFMSKVIAGASK |
Function
Involved in pre-mRNA splicing. Remains associated with spliced mRNA after splicing which probably involves interactions with the exon junction complex (EJC). Can trigger mRNA decay which seems to be independent of nonsense-mediated decay involving premature stop codons (PTC) recognition. May be involved in nuclear mRNA decay. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45 is proposed to sequester phosphorylated SFPQ from PTPRC/CD45 pre-mRNA in resting T-cells. Involved in cyclin-D1/CCND1 mRNA stability probably by acting as component of the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. Involved in response to DNA damage. Is excluced from DNA damage sites in a manner that parallels transcription inhibition; the function may involve the SNARP complex. Initially thought to play a role in transcriptional coactivation through its association with the TRAP complex; however, it is not regarded as a stable Mediator complex subunit. Cooperatively with HELZ2, enhances the transcriptional activation mediated by PPARG, maybe through the stabilization of the PPARG binding to DNA in presence of ligand. May play a role in the terminal stage of adipocyte differentiation. Plays a role in the positive regulation of the circadian clock. Acts as a coactivator of the CLOCK-BMAL1 heterodimer and promotes its transcriptional activator activity and binding to circadian target genes (PubMed:24043798)
Protein Sequence
10
MSKTNKSKSG
20
SRSSRSRSAS
30
RSRSRSFSKS
40
RSRSRSVSRS
50
RKRRLSSRSR
60
SRSYSPAHNR
70
ERNHPRVYQN
80
RDFRGHNRGY
90
RRPYYFRGRN
100
RGFYPWGQYN
110
RGGYGNYRSN
120
WQNYRQAYSP
130
RRGRSRSRSP
140
KRRSPSPRSR
150
SHSRNSDKSS
160
SDRSRRSSSS
170
RSSSNHSRVE
180
SSKRKSTKEK
190
KSSSKDSRPS
200
QAAGDNQGDE
210
AKEQTFSGGT
220
SQDIKGSESS
230
KPWPDATTYG
240
AGSASRASVS
250
DLSPRERSPA
260
LKSPLQSVVV
270
RRRSPRPSPV
280
PKPSPPLSNA
290
SQMGSSMSGG
300
AGYQSGAHQG
310
QFDHGSGSLS
320
PSKKSPVGKS
330
PPATGSAYGS
340
SQKEESAASG
350
GAAYSKRYLE
360
EQKTENGKDK
370
EQKQTNADKE
380
KLKEKGGFSD
390
ADVKMKSDPF
400
APKTDSEKPF
410
RGSQSPKRYK
420
LRDDFEKKMA
430
DFHKEELDEH
440
DKDKSKGRKE
450
PEFDDEPKFM
460
SKVIAGASKN
470
QEEEKSGKWE
480
SLHTGKEKQR
490
KAEEMEDEPF
500
TERSRKEERG
510
GSKRSESGHR
520
GFVPEKNFRV
530
TAYKAVQEKS
540
SSPPPRKTSE
550
SRDKLGSKGD
560
FSSGKSSFSI
570
TREAQVNVRM
580
DSFDEDLARP
590
SGLLAQERKL
600
CRDLVHSNKK
610
EQEFRSIFQH
620
IQSAQSQRSP
630
SELFAQHIVT
640
IVHHVKEHHF
650
GSSGMTLHER
660
FTKYLKRGNE
670
QEAAKNKKSP
680
EIHRRIDISP
690
STFRKHGLTH
700
EELKSPREPG
710
YKAEGKYKDD
720
PVDLRLDIER
730
RKKHKERDLK
740
RGKSRESVDS
750
RDSSHSRERS
760
TEKTEKTHKG
770
SKKQKKHRRA
780
RDRSRSSSSS
790
SQSSHSYKAE
800
EYPEEAEERE
810
ESTSGFDKSR
820
LGTKDFVGPN
830
ERGGRARGTF
840
QFRARGRGWG
850
RGNYSGNNNN
860
NSNNDFQKRS
870
REEEWDPEYT
880
PKSKKYYLHD
890
DREGEGSDKW
900
MGRGRGRGAF
910
PRGRGRFMFR
920
KSSTSPKWAH
930
DKFSGEEGEI
940
EDDESGTENR
950
EEKDSLQPSA
E
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0035145 |
exon-exon junction complex |
| Biological Process |
GO:0000956 |
nuclear-transcribed mRNA catabolic process |
| Biological Process |
GO:0042753 |
positive regulation of circadian rhythm |
| Biological Process |
GO:0045893 |
positive regulation of DNA-templated transcription |
| Biological Process |
GO:0048026 |
positive regulation of mRNA splicing, via spliceosome |
| Biological Process |
GO:0045944 |
positive regulation of transcription by RNA polymerase II |
| Biological Process |
GO:0000381 |
regulation of alternative mRNA splicing, via spliceosome |
| Biological Process |
GO:0008380 |
RNA splicing |
| Biological Process |
GO:0006366 |
transcription by RNA polymerase II |
| Cellular Component |
GO:0016592 |
mediator complex |
| Cellular Component |
GO:0016607 |
nuclear speck |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:0046966 |
nuclear thyroid hormone receptor binding |
| Molecular Function |
GO:0051219 |
phosphoprotein binding |
| Molecular Function |
GO:0000978 |
RNA polymerase II cis-regulatory region sequence-specific DNA binding |
| Molecular Function |
GO:0003713 |
transcription coactivator activity |
| Molecular Function |
GO:0003712 |
transcription coregulator activity |
| Biological Process |
GO:0007623 |
circadian rhythm |
| Biological Process |
GO:0006397 |
mRNA processing |
| Biological Process |
GO:0048255 |
mRNA stabilization |
Reference
[1] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.