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Overview

Uniprot IDQ570Y9
Protein NameDEP domain-containing mTOR-interacting protein
Gene NameDeptor
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
48 RLRLHEEKVIKDRRH

Function

Negative regulator of the mTORC1 and mTORC2 complexes: inhibits the protein kinase activity of MTOR, thereby inactivating both complexes. DEPTOR inhibits mTORC1 and mTORC2 to induce autophagy. In contrast to AKT1S1/PRAS40, only partially inhibits mTORC1 activity

Protein Sequence

10 MEEGSSGGSG 20 SSDSNAGGSG 30 GVQQRELERM 40 AEVLVTGEQL 50 RLRLHEEKVI 60 KDRRHHLKTY 70 PNCFVAKELI 80 DWLIEHKEAS 90 DRETAIKLMQ 100 KLADRGIIHH 110 VCDEHKEFKD 120 VKLFYRFRKD 130 DGTFALDSEV 140 KAFMRGQRLY 150 EKLMSPETTL 160 LQPREEEGVK 170 YERTFMASEF 180 LDWLVQEGEA 190 TTRKEAEQLC 200 HRLMDHGIIQ 210 HVSNKHPFVD 220 SNLLYQFRMN 230 FRRRRRLMEL 240 LNETSPSSQE 250 THDSPFCLRK 260 QSHDSRKSTS 270 FMSVSPSKEI 280 KIVSAVRRSS 290 MSSCGSSGYF 300 SSSPTLSSSP 310 PVLCNPKSVL 320 KRPVTSEELL 330 TPGAPYARKT 340 FTIVGDAVGW 350 GFVVRGSKPC 360 HIQAVDPSGP 370 AAAAGMKVCQ 380 FVVSVNGLNV 390 LNVDYRTVSN 400 LILTGPRTIV MEVMEELDC

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005765 lysosomal membrane
Cellular Component GO:0005886 plasma membrane
Molecular Function GO:0005096 GTPase activator activity
Molecular Function GO:0005085 guanyl-nucleotide exchange factor activity
Molecular Function GO:0070300 phosphatidic acid binding
Molecular Function GO:0004860 protein kinase inhibitor activity
Molecular Function GO:0030291 protein serine/threonine kinase inhibitor activity
Biological Process GO:0000045 autophagosome assembly
Biological Process GO:0031669 cellular response to nutrient levels
Biological Process GO:0007186 G protein-coupled receptor signaling pathway
Biological Process GO:0010507 negative regulation of autophagy
Biological Process GO:0045792 negative regulation of cell size
Biological Process GO:0006469 negative regulation of protein kinase activity
Biological Process GO:0032007 negative regulation of TOR signaling
Biological Process GO:1904262 negative regulation of TORC1 signaling
Biological Process GO:1903940 negative regulation of TORC2 signaling
Biological Process GO:0010508 positive regulation of autophagy
Biological Process GO:2001236 regulation of extrinsic apoptotic signaling pathway
Biological Process GO:0038202 TORC1 signaling

Reference

[1] Chang J, Wu W, Qian P, Lu Z, He X et al.. Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue.. Front Cell Dev Biol 12:1472338. 2024. PMID: 39935788.