Overview
| Uniprot ID | Q5BJT0 |
|---|
| Protein Name | Arginine and glutamate-rich protein 1 |
|---|
| Gene Name | Arglu1 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 251 |
RQQKEEQKIILGKGK |
| 262 |
GKGKSRPKLSFSLKT |
Function
Dual function regulator of gene expression; regulator of transcription and modulator of alternative splicing. General coactivator of nuclear receptor-induced gene expression, including genes activated by the glucocorticoid receptor NR3C1. Binds to a subset of pre-mRNAs and to components of the spliceosome machinery to directly modulate basal alternative splicing; involved in simple and complex cassette exon splicing events (By similarity). Binds its own pre-mRNA and regulates its alternative splicing and degradation; one of the alternatively spliced products is a stable intronic sequence RNA (sisRNA) that binds the protein to regulate its ability to affect splicing (By similarity). Binding of the sisRNA stimulates phase separation and localization to nuclear speckles, which may contribute to activation of nuclear receptor-induced gene expression (By similarity). May also indirectly modulate alternative splicing (By similarity). Regulates transcription of genes involved in heart development, neuronal cell function, protein localization and chromatin localization. Regulates splicing of genes involved in neurogenesis and chromatin organization. Essential for central nervous system development (By similarity). Required for the estrogen-dependent expression of ESR1 target genes. Can act in cooperation with MED1 (By similarity)
Protein Sequence
10
MGRSRSRSSS
20
RSKHTKSSKH
30
NKKRSRSRSR
40
SRDKERVRKR
50
SKSRESKRNR
60
RRESRSRSRS
70
TNAAASRRER
80
ERASSPPDRI
90
DIFGRTVSKR
100
SSLDEKQKRE
110
EEEKKAEFER
120
QRKIRQQEIE
130
EKLIEEETAR
140
RVEELVAKRV
150
EEELEKRKDE
160
IEREVLRRVE
170
EAKRIMEKQL
180
LEELERQRQA
190
ELAAQKAREE
200
EERAKREELE
210
RILEENNRKI
220
AEAQAKLAEE
230
QLRIVEEQRK
240
IHEERMKLEQ
250
ERQRQQKEEQ
260
KIILGKGKSR
270
PKLSFSLKTQ
D
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005694 |
chromosome |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Cellular Component |
GO:0016607 |
nuclear speck |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Molecular Function |
GO:0036002 |
pre-mRNA binding |
| Molecular Function |
GO:0003713 |
transcription coactivator activity |
| Biological Process |
GO:0006397 |
mRNA processing |
| Biological Process |
GO:0000381 |
regulation of alternative mRNA splicing, via spliceosome |
| Biological Process |
GO:0008380 |
RNA splicing |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.