Search Results

Overview

Uniprot IDQ5FVM4
Protein NameNon-POU domain-containing octamer-binding protein
Gene NameNono
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
11 NKTFNLEKQNHTPRK
195 DRGRPSGKGIVEFSG
248 LPEKLVIKNQQFHKE
254 IKNQQFHKEREQPPR
341 LHNQEVQKRKQLELR
376 RRQQEGFKGTFPDAR
472 GAEFAPNKRRRY***
5 ***MQSNKTFNLEKQ

Function

DNA- and RNA binding protein, involved in several nuclear processes (By similarity). Binds the conventional octamer sequence in double-stranded DNA (By similarity). Also binds single-stranded DNA and RNA at a site independent of the duplex site (By similarity). Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ (By similarity). Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b (By similarity). Together with PSPC1, required for the formation of nuclear paraspeckles (By similarity). The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs (By similarity). The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 (By similarity). The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends (By similarity). In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex (By similarity). NONO is involved in transcriptional regulation (By similarity). The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity (By similarity). NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription (By similarity). Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer (PubMed:15860628). Important for the functional organization of GABAergic synapses (By similarity). Plays a specific and important role in the regulation of synaptic RNAs and GPHN/gephyrin scaffold structure, through the regulation of GABRA2 transcript (By similarity). Plays a key role during neuronal differentiation by recruiting TET1 to genomic loci and thereby regulating 5-hydroxymethylcytosine levels (By similarity). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (By similarity)

Protein Sequence

10 MQSNKTFNLE 20 KQNHTPRKHH 30 QHHHQQHHQQ 40 QQQQQQQQQQ 50 QPPPPIPANG 60 QQASSQNEGL 70 TIDLKNFRKP 80 GEKTFTQRSR 90 LFVGNLPPDI 100 TEEEMRKLFE 110 KYGKAGEVFI 120 HKDKGFGFIR 130 LETRTLAEIA 140 KVELDNMPLR 150 GKQLRVRFAC 160 HSASLTVRNL 170 PQYVSNELLE 180 EAFSVFGQVE 190 RAVVIVDDRG 200 RPSGKGIVEF 210 SGKPAARKAL 220 DRCSEGSFLL 230 TTFPRPVTVE 240 PMDQLDDEEG 250 LPEKLVIKNQ 260 QFHKEREQPP 270 RFAQPGSFEY 280 EYAMRWKALI 290 EMEKQQQDQV 300 DRNIKEAREK 310 LEMEMEAARH 320 EHQVMLMRQD 330 LMRRQEELRR 340 MEELHNQEVQ 350 KRKQLELRQE 360 EERRRREEEM 370 RRQQEEMMRR 380 QQEGFKGTFP 390 DAREQEIRMG 400 QMAMGGAMGI 410 NNRGAMPPAP 420 VPPGTPAPPG 430 PAAMMPDGTL 440 GLTPPTTERF 450 GQAATMEGIG 460 AIGGTPPAFN 470 RPAPGAEFAP NKRRRY

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005694 chromosome
Cellular Component GO:0001650 fibrillar center
Cellular Component GO:0016363 nuclear matrix
Cellular Component GO:0016607 nuclear speck
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0042382 paraspeckles
Cellular Component GO:0090575 RNA polymerase II transcription regulator complex
Molecular Function GO:0003682 chromatin binding
Molecular Function GO:0003677 DNA binding
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0106222 lncRNA binding
Molecular Function GO:0003723 RNA binding
Biological Process GO:0002218 activation of innate immune response
Biological Process GO:1904385 cellular response to angiotensin
Biological Process GO:0071456 cellular response to hypoxia
Biological Process GO:0007623 circadian rhythm
Biological Process GO:0006310 DNA recombination
Biological Process GO:0006281 DNA repair
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0045087 innate immune response
Biological Process GO:0006397 mRNA processing
Biological Process GO:0043066 negative regulation of apoptotic process
Biological Process GO:0045892 negative regulation of DNA-templated transcription
Biological Process GO:1903377 negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway
Biological Process GO:0042752 regulation of circadian rhythm
Biological Process GO:0006355 regulation of DNA-templated transcription
Biological Process GO:0008380 RNA splicing

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.