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Overview

Uniprot ID	Q5GFD9
Protein Name	Protein IMPACT
Gene Name	Impact
Organism	Rattus norvegicus

Kla Sites from experimental identification

Position	Flanking peptide
116	IRDALIQKSQITEPG
301	NSPEESAKSFGKKKV

Function

Translational regulator that ensures constant high levels of translation upon a variety of stress conditions, such as amino acid starvation, UV-C irradiation, proteasome inhibitor treatment and glucose deprivation. Plays a role as a negative regulator of the EIF2AK4/GCN2 kinase activity; impairs GCN1-mediated EIF2AK4/GCN2 activation, and hence EIF2AK4/GCN2-mediated eIF-2-alpha phosphorylation and subsequent down-regulation of protein synthesis. May be required to regulate translation in specific neuronal cells under amino acid starvation conditions by preventing GCN2 activation and therefore ATF4 synthesis. Through its inhibitory action on EIF2AK4/GCN2, plays a role in differentiation of neuronal cells by stimulating neurite outgrowth

Protein Sequence

10 MAEVESGSNQ 20 RQNEEIEAMA 30 AIYGEEWCVI 40 DEVAKIFCIR 50 ITDDMDDPKW 60 TLCLQVMLPS 70 EYPGTAPPVY 80 QLNAPWLKGQ 90 ERAELAKSLE 100 EIYMKNIGES 110 ILYQWVEKIR 120 DALIQKSQIT 130 EPGPDEKKKT 140 EEEEVEGEDD 150 PILEHPPENP 160 VKTWDLKISE 170 SAPEAEELPP 180 IAHGAPITDR 190 RSTFQAHLAP 200 VVCIDQVKKV 210 LAKLYENKKI 220 ASATHNIYAY 230 RIYCEDKQTF 240 LQDSEDDGET 250 AAGGRLLHLM 260 EILNVKNVMV 270 VVSRWYGGIL 280 LGPDRFKHIN 290 NCARNILVEK 300 NFTNSPEESA 310 KSFGKKKVKK DKKKGDH

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005737	cytoplasm
Molecular Function	GO:0003779	actin binding
Molecular Function	GO:0140311	protein sequestering activity
Molecular Function	GO:0043022	ribosome binding
Biological Process	GO:0034198	cellular response to amino acid starvation
Biological Process	GO:0140469	GCN2-mediated signaling
Biological Process	GO:0035556	intracellular signal transduction
Biological Process	GO:0001933	negative regulation of protein phosphorylation
Biological Process	GO:0000122	negative regulation of transcription by RNA polymerase II
Biological Process	GO:1990138	neuron projection extension
Biological Process	GO:0045666	positive regulation of neuron differentiation
Biological Process	GO:1990611	regulation of cytoplasmic translational initiation in response to stress
Biological Process	GO:0006446	regulation of translational initiation

Reference

[1] Yao Y, Bade R, Li G, Zhang A, Zhao H et al.. Global-Scale Profiling of Differential Expressed Lysine-Lactylated Proteins in the Cerebral Endothelium of Cerebral Ischemia-Reperfusion Injury Rats.. Cell Mol Neurobiol 43(5):1989-2004. 2023 Jul. PMID: 36030297.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.