Overview
| Uniprot ID | Q5I0K3 |
|---|
| Protein Name | Citramalyl-CoA lyase, mitochondrial |
|---|
| Gene Name | Clybl |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 80 |
EDGVAENKKNEARLR |
Function
Mitochondrial enzyme required to detoxify vitamin B12-poisoning metabolites. Acts as a citramalyl-CoA lyase by converting citramalyl-CoA into acetyl-CoA and pyruvate in the C5-dicarboxylate catabolism pathway, a pathway required to detoxify itaconate, a vitamin B12-poisoning metabolite. Also acts as a malyl-CoA thioesterase to detoxify malyl-CoA, a side product of citric acid cycle enzymes, which is toxic for the vitamin B12-dependent enzyme MMUT. Also acts as a malate synthase in vitro, converting glyoxylate and acetyl-CoA to malate. Also acts as a beta-methylmalate synthase in vitro, by mediating conversion of glyoxylate and propionyl-CoA to beta-methylmalate. Also has very weak citramalate synthase activity in vitro
Protein Sequence
10
MALCVLQNAV
20
RGAAALPRLK
30
ASLVASVCRP
40
GYSSLSNHKY
50
VPRRAVLYVP
60
GNDEKKIRKI
70
PSLKVDCAVL
80
DCEDGVAENK
90
KNEARLRIAK
100
TLEDFDLGTT
110
EKCVRINSVS
120
SGLAEADLET
130
FLQARVLPSS
140
LMLPKVEGPE
150
EIQWFSDKFS
160
LHLKGRKLEQ
170
PMNLIPFVET
180
AMGLLNFKAV
190
CEETLKIGPQ
200
VGLFLDAVVF
210
GGEDFRASIG
220
ATSNKDTQDI
230
LYARQKIVVT
240
AKAFGLQAID
250
LVYIDFRDED
260
GLLRQSREAA
270
AMGFTGKQVI
280
HPNQIAVVQE
290
QFTPTPEKIR
300
WAEELIAAFK
310
EHQQLGKGAF
320
TFQGSMIDMP
330
LLKQAQNIVT
LATSIKEK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Molecular Function |
GO:0047777 |
(S)-citramalyl-CoA lyase activity |
| Molecular Function |
GO:0016289 |
acyl-CoA hydrolase activity |
| Molecular Function |
GO:0000287 |
magnesium ion binding |
| Molecular Function |
GO:0004474 |
malate synthase activity |
| Biological Process |
GO:0106121 |
positive regulation of cobalamin metabolic process |
| Biological Process |
GO:0070207 |
protein homotrimerization |
| Biological Process |
GO:0106064 |
regulation of cobalamin metabolic process |
| Biological Process |
GO:0110052 |
toxic metabolite repair |
Reference
[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.