Search Results

Overview

Uniprot IDQ5JRA6
Protein NameTransport and Golgi organization protein 1 homolog
Gene NameMIA3
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1485 LRASVSTKCNLEDQV
1537 KEMALQKKLSQEEYE
1558 RLSAADEKAVSAAEE
1588 QKTERSFKNQIATHE

Function

Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. This protein is required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. It may participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. It is also specifically required for the secretion of lipoproteins by participating in their export from the endoplasmic reticulum (PubMed:19269366, PubMed:27138255). Required for correct assembly of COPII coat components at endoplasmic reticulum exit sites (ERES) and for the localization of SEC16A and membrane-bound ER-resident complexes consisting of MIA2 and PREB/SEC12 to ERES (PubMed:28442536)

Protein Sequence

10 MAAAPGLLVW 20 LLVLRLPWRV 30 PGQLDPSTGR 40 RFSEHKLCAD 50 DECSMLMYRG 60 EALEDFTGPD 70 CRFVNFKKGD 80 PVYVYYKLAR 90 GWPEVWAGSV 100 GRTFGYFPKD 110 LIQVVHEYTK 120 EELQVPTDET 130 DFVCFDGGRD 140 DFHNYNVEEL 150 LGFLELYNSA 160 ATDSEKAVEK 170 TLQDMEKNPE 180 LSKEREPEPE 190 PVEANSEESD 200 SVFSENTEDL 210 QEQFTTQKHH 220 SHANSQANHA 230 QGEQASFESF 240 EEMLQDKLKV 250 PESENNKTSN 260 SSQVSNEQDK 270 IDAYKLLKKE 280 MTLDLKTKFG 290 STADALVSDD 300 ETTRLVTSLE 310 DDFDEELDTE 320 YYAVGKEDEE 330 NQEDFDELPL 340 LTFTDGEDMK 350 TPAKSGVEKY 360 PTDKEQNSNE 370 EDKVQLTVPP 380 GIKNDDKNIL 390 TTWGDTIFSI 400 VTGGEETRDT 410 MDLESSSSEE 420 EKEDDDDALV 430 PDSKQGKPQS 440 ATDYSDPDNV 450 DDGLFIVDIP 460 KTNNDKEVNA 470 EHHIKGKGRG 480 VQESKRGLVQ 490 DKTELEDENQ 500 EGMTVHSSVH 510 SNNLNSMPAA 520 EKGKDTLKSA 530 YDDTENDLKG 540 AAIHISKGML 550 HEEKPGEQIL 560 EGGSESESAQ 570 KAAGNQMNDR 580 KIQQESLGSA 590 PLMGDDHPNA 600 SRDSVEGDAL 610 VNGAKLHTLS 620 VEHQREELKE 630 ELVLKTQNQP 640 RFSSPDEIDL 650 PRELEDEVPI 660 LGRNLPWQQE 670 RDVAATASKQ 680 MSEKIRLSEG 690 EAKEDSLDEE 700 FFHHKAMQGT 710 EVGQTDQTDS 720 TGGPAFLSKV 730 EEDDYPSEEL 740 LEDENAINAK 750 RSKEKNPGNQ 760 GRQFDVNLQV 770 PDRAVLGTIH 780 PDPEIEESKQ 790 ETSMILDSEK 800 TSETAAKGVN 810 TGGREPNTMV 820 EKERPLADKK 830 AQRPFERSDF 840 SDSIKIQTPE 850 LGEVFQNKDS 860 DYLKNDNPEE 870 HLKTSGLAGE 880 PEGELSKEDH 890 ENTEKYMGTE 900 SQGSAAAEPE 910 DDSFHWTPHT 920 SVEPGHSDKR 930 EDLLIISSFF 940 KEQQSLQRFQ 950 KYFNVHELEA 960 LLQEMSSKLK 970 SAQQESLPYN 980 MEKVLDKVFR 990 ASESQILSIA 1000 EKMLDTRVAE 1010 NRDLGMNENN 1020 IFEEAAVLDD 1030 IQDLIYFVRY 1040 KHSTAEETAT 1050 LVMAPPLEEG 1060 LGGAMEEMQP 1070 LHEDNFSREK 1080 TAELNVQVPE 1090 EPTHLDQRVI 1100 GDTHASEVSQ 1110 KPNTEKDLDP 1120 GPVTTEDTPM 1130 DAIDANKQPE 1140 TAAEEPASVT 1150 PLENAILLIY 1160 SFMFYLTKSL 1170 VATLPDDVQP 1180 GPDFYGLPWK 1190 PVFITAFLGI 1200 ASFAIFLWRT 1210 VLVVKDRVYQ 1220 VTEQQISEKL 1230 KTIMKENTEL 1240 VQKLSNYEQK 1250 IKESKKHVQE 1260 TRKQNMILSD 1270 EAIKYKDKIK 1280 TLEKNQEILD 1290 DTAKNLRVML 1300 ESEREQNVKN 1310 QDLISENKKS 1320 IEKLKDVISM 1330 NASEFSEVQI 1340 ALNEAKLSEE 1350 KVKSECHRVQ 1360 EENARLKKKK 1370 EQLQQEIEDW 1380 SKLHAELSEQ 1390 IKSFEKSQKD 1400 LEVALTHKDD 1410 NINALTNCIT 1420 QLNLLECESE 1430 SEGQNKGGND 1440 SDELANGEVG 1450 GDRNEKMKNQ 1460 IKQMMDVSRT 1470 QTAISVVEED 1480 LKLLQLKLRA 1490 SVSTKCNLED 1500 QVKKLEDDRN 1510 SLQAAKAGLE 1520 DECKTLRQKV 1530 EILNELYQQK 1540 EMALQKKLSQ 1550 EEYERQEREH 1560 RLSAADEKAV 1570 SAAEEVKTYK 1580 RRIEEMEDEL 1590 QKTERSFKNQ 1600 IATHEKKAHE 1610 NWLKARAAER 1620 AIAEEKREAA 1630 NLRHKLLELT 1640 QKMAMLQEEP 1650 VIVKPMPGKP 1660 NTQNPPRRGP 1670 LSQNGSFGPS 1680 PVSGGECSPP 1690 LTVEPPVRPL 1700 SATLNRRDMP 1710 RSEFGSVDGP 1720 LPHPRWSAEA 1730 SGKPSPSDPG 1740 SGTATMMNSS 1750 SRGSSPTRVL 1760 DEGKVNMAPK 1770 GPPPFPGVPL 1780 MSTPMGGPVP 1790 PPIRYGPPPQ 1800 LCGPFGPRPL 1810 PPPFGPGMRP 1820 PLGLREFAPG 1830 VPPGRRDLPL 1840 HPRGFLPGHA 1850 PFRPLGSLGP 1860 REYFIPGTRL 1870 PPPTHGPQEY 1880 PPPPAVRDLL 1890 PSGSRDEPPP 1900 ASQSTSQDCS QALKQSP

Gene Ontology

Classification GO ID Description
Biological Process GO:0007029 endoplasmic reticulum organization
Cellular Component GO:0070971 endoplasmic reticulum exit site
Cellular Component GO:0005788 endoplasmic reticulum lumen
Cellular Component GO:0005789 endoplasmic reticulum membrane
Cellular Component GO:0016020 membrane
Molecular Function GO:0038024 cargo receptor activity
Biological Process GO:0002042 cell migration involved in sprouting angiogenesis
Biological Process GO:0140052 cellular response to oxidised low-density lipoprotein particle stimulus
Biological Process GO:0090110 COPII-coated vesicle cargo loading
Biological Process GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport
Biological Process GO:0006887 exocytosis
Biological Process GO:0042953 lipoprotein transport
Biological Process GO:0007162 negative regulation of cell adhesion
Biological Process GO:0030336 negative regulation of cell migration
Biological Process GO:1903038 negative regulation of leukocyte cell-cell adhesion
Biological Process GO:2000402 negative regulation of lymphocyte migration
Biological Process GO:0002687 positive regulation of leukocyte migration
Biological Process GO:0070973 protein localization to endoplasmic reticulum exit site
Biological Process GO:0009306 protein secretion
Biological Process GO:0015031 protein transport
Biological Process GO:0035459 vesicle cargo loading
Biological Process GO:0042060 wound healing

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.