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Overview

Uniprot ID	Q5JSH3
Protein Name	WD repeat-containing protein 44
Gene Name	WDR44
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
261	APPPRKRKSELEFET
440	GKSVKRAKHLAEEYG

Function

Downstream effector for Rab11 which regulates Rab11 intracellular membrane trafficking functions such as endocytic recycling, intracellular ciliogenesis and protein export (PubMed:31204173, PubMed:32344433). ATK1-mediated phosphorylation of WDR44 induces binding to Rab11 which activates endocytic recycling of transferrin receptor back to the plasma membrane (PubMed:31204173). When bound to Rab11, prevents the formation of the ciliogenic Rab11-Rabin8/RAB3IP-RAB11FIP3 complex, therefore inhibiting preciliary trafficking and ciliogenesis (PubMed:31204173). Participates in neo-synthesized protein export by connecting the endoplasmic reticulum (ER) with the endosomal tubule via direct interactions with the integral ER proteins VAPA or VAPB and the endosomal protein GRAFs (GRAF1/ARHGAP26 or GRAF2/ARHGAP10), which facilitates the transfer of proteins such as E-cadherin, MPP14 and CFTR into a Rab8-Rab10-Rab11-dependent export route (PubMed:32344433)

Protein Sequence

10 MASESDTEEF 20 YDAPEDVHLG 30 GGYPVGSPGK 40 VGLSTFKETE 50 NTAYKVGNES 60 PVQELKQDVS 70 KKIIESIIEE 80 SQKVLQLEDD 90 SLDSKGKELS 100 DQATASPIVA 110 RTDLSNIPGL 120 LAIDQVLPEE 130 SQKAESQNTF 140 EETELELKKC 150 FPSDETCEKP 160 VDETTKLTQT 170 SSTEQLNVLE 180 TETEVLNKEA 190 VEVKGGGDVL 200 EPVSSDSLST 210 KDFAAVEEVA 220 PAKPPRHLTP 230 EPDIVASTKK 240 PVPARPPPPT 250 NFPPPRPPPP 260 SRPAPPPRKR 270 KSELEFETLK 280 TPDIDVPKEN 290 ITSDSLLTAS 300 MASESTVKDS 310 QPSLDLASAT 320 SGDKIVTAQE 330 NGKAPDGQTV 340 AGEVMGPQRP 350 RSNSGRELTD 360 EEILASVMIK 370 NLDTGEEIPL 380 SLAEEKLPTG 390 INPLTLHIMR 400 RTKEYVSNDA 410 AQSDDEEKLQ 420 SQPTDTDGGR 430 LKQKTTQLKK 440 FLGKSVKRAK 450 HLAEEYGERA 460 INKVKSVRDE 470 VFHTDQDDPS 480 SSDDEGMPYT 490 RPVKFKAAHG 500 FKGPYDFDQI 510 KVVQDLSGEH 520 MGAVWTMKFS 530 HCGRLLASAG 540 QDNVVRIWAL 550 KNAFDYFNNM 560 RMKYNTEGRV 570 SPSPSQESLS 580 SSKSDTDTGV 590 CSGTDEDPDD 600 KNAPFRQRPF 610 CKYKGHTADL 620 LDLSWSKNYF 630 LLSSSMDKTV 640 RLWHISRREC 650 LCCFQHIDFV 660 TAIAFHPRDD 670 RYFLSGSLDG 680 KLRLWNIPDK 690 KVALWNEVDG 700 QTKLITAANF 710 CQNGKYAVIG 720 TYDGRCIFYD 730 TEHLKYHTQI 740 HVRSTRGRNK 750 VGRKITGIEP 760 LPGENKILVT 770 SNDSRIRLYD 780 LRDLSLSMKY 790 KGYVNSSSQI 800 KASFSHDFTY 810 LVSGSEDKYV 820 YIWSTYHDLS 830 KFTSVRRDRN 840 DFWEGIKAHN 850 AVVTSAIFAP 860 NPSLMLSLDV 870 QSEKSEGNEK 880 SEDAEVLDAT 890 PSGIMKTDNT 900 EVLLSADFTG 910 AIKVFVNKRK NVS

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0010008	endosome membrane
Cellular Component	GO:0005794	Golgi apparatus
Cellular Component	GO:0048471	perinuclear region of cytoplasm
Molecular Function	GO:0140313	molecular sequestering activity
Molecular Function	GO:0031267	small GTPase binding
Biological Process	GO:0061824	cytosolic ciliogenesis
Biological Process	GO:1902018	negative regulation of cilium assembly
Biological Process	GO:0060627	regulation of vesicle-mediated transport

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[5] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.