Overview
| Uniprot ID | Q5M7A4 |
|---|
| Protein Name | Ubiquitin-like modifier-activating enzyme 5 |
|---|
| Gene Name | Uba5 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 40 |
CGRTRIQKMSDEVVD |
Function
E1-like enzyme which specifically catalyzes the first step in ufmylation. Activates UFM1 by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a UFM1-E1 thioester and free AMP. Activates UFM1 via a trans-binding mechanism, in which UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer. Trans-binding also promotes stabilization of the UBA5 homodimer, and enhances ATP-binding. Transfer of UFM1 from UBA5 to the E2-like enzyme UFC1 also takes place using a trans mechanism. Ufmylation plays a key role in various processes, such as ribosome recycling, response to DNA damage, interferon response or reticulophagy (also called ER-phagy) (By similarity). Ufmylation is essential for erythroid differentiation of both megakaryocytes and erythrocytes (By similarity)
Protein Sequence
10
MAESVERLLQ
20
RVEELEQELA
30
RERSRRIAGD
40
GHCGRTRIQK
50
MSDEVVDSNP
60
YSRLMALKRM
70
GVVSDYEKIR
80
TYAVAIVGVG
90
GVGSVTAEML
100
TRCGIGKLLL
110
FDYDKVELAN
120
MNRLFFQPYQ
130
AGMSKVQAAE
140
HTLRSINPDV
150
LFEVHNYNIT
160
TVEHFEHFMN
170
RISNGGLEEG
180
QPVDLVLSCV
190
DNFEARMAIN
200
TACNELGQTW
210
MESGVSENAV
220
SGHIQLMVPG
230
ESACFACAPP
240
LVVASNIDEK
250
TLKREGVCAA
260
SLPTTMGVVA
270
GILVQNVLKF
280
LLKFGTVSFY
290
LGYNAMQDFF
300
PTMFMKPNPQ
310
CDDKNCRKQQ
320
EEYKKRAPAQ
330
PTQETAPQEE
340
EEVVHEDNEW
350
GIELVSEVSE
360
EELKNSSGPV
370
PTLPEGITVA
380
YTVPKKREDS
390
VSEVTVEDSG
400
ESLEDLMARM
KKM
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005789 |
endoplasmic reticulum membrane |
| Cellular Component |
GO:0005794 |
Golgi apparatus |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0042803 |
protein homodimerization activity |
| Molecular Function |
GO:0071566 |
UFM1 activating enzyme activity |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0030218 |
erythrocyte differentiation |
| Biological Process |
GO:0030219 |
megakaryocyte differentiation |
| Biological Process |
GO:0050905 |
neuromuscular process |
| Biological Process |
GO:1990592 |
protein K69-linked ufmylation |
| Biological Process |
GO:0071569 |
protein ufmylation |
| Biological Process |
GO:0033146 |
regulation of intracellular estrogen receptor signaling pathway |
| Biological Process |
GO:0032649 |
regulation of type II interferon production |
| Biological Process |
GO:0034976 |
response to endoplasmic reticulum stress |
| Biological Process |
GO:0061709 |
reticulophagy |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.