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Overview

Uniprot IDQ5M7V8
Protein NameThyroid hormone receptor-associated protein 3
Gene NameThrap3
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
252 RERSPALKSPLQSVV
346 SGGAAYTKRYLEEQK
375 DKEKLKEKGGFSDAD
384 GFSDADVKMKSDPFA
386 SDADVKMKSDPFAPK
418 LRDDFEKKMADFHKE
439 KDKCKGRKEPEFDDE
452 DEPKFISKVIAGASK
459 KVIAGASKNQEEEKS
468 QEEEKSGKWESLHAG
476 WESLHAGKEKQRKAE
481 AGKEKQRKAEELEEE
516 HRGFVPEKNFRVTAY
524 NFRVTAYKAVQEKSS
548 SRDKLGNKGDFSSGK
555 KGDFSSGKSSFSITR
599 RDLVHSNKKEQEFRS
685 ISPSTFRKHGLTHEE
694 GLTHEELKSPREPGY
702 SPREPGYKAEGKYKD
706 PGYKAEGKYKDDPVD
708 YKAEGKYKDDPVDLR
872 WDPEYTPKSKKYYLH
875 EYTPKSKKYYLHDDR
917 RKSSTSPKWAHDKFS

Function

Involved in pre-mRNA splicing. Remains associated with spliced mRNA after splicing which probably involves interactions with the exon junction complex (EJC). Can trigger mRNA decay which seems to be independent of nonsense-mediated decay involving premature stop codons (PTC) recognition. May be involved in nuclear mRNA decay. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45 is proposed to sequester phosphorylated SFPQ from PTPRC/CD45 pre-mRNA in resting T-cells. Involved in cyclin-D1/CCND1 mRNA stability probably by acting as component of the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. Involved in response to DNA damage. Is excluced from DNA damage sites in a manner that parallels transcription inhibition; the function may involve the SNARP complex. Initially thought to play a role in transcriptional coactivation through its association with the TRAP complex; however, it is not regarded as a stable Mediator complex subunit. Cooperatively with HELZ2, enhances the transcriptional activation mediated by PPARG, maybe through the stabilization of the PPARG binding to DNA in presence of ligand. May play a role in the terminal stage of adipocyte differentiation. Plays a role in the positive regulation of the circadian clock. Acts as a coactivator of the CLOCK-BMAL1 heterodimer and promotes its transcriptional activator activity and binding to circadian target genes

Protein Sequence

10 MSKTNKSKSG 20 SRSSRSRSAS 30 RSRSRSFSKS 40 RSRSRSVSRS 50 RKRRLSSRSR 60 SRSYSPAHNR 70 ERNHPRVYQN 80 RDFRGHNRGY 90 RRPYYFRGRN 100 RGFYPWGQYN 110 RGGYGNYRSN 120 WQNYRQAYSP 130 RRGRSRSRSP 140 KRRSPSPRSR 150 SHSRNSDKSS 160 SDRSRRSSSS 170 RSSSNHSRVE 180 SSKRKSTKEK 190 KSSSKDSRPS 200 QAAGDNQGDE 210 AKEQTFSGGT 220 SQDIKGSESS 230 KPWPDATTYG 240 TGSASRASVS 250 DLSPRERSPA 260 LKSPLQSVVV 270 RRRSPRPSPV 280 PKPSPPLSNA 290 SQMGSSMSGG 300 AGYQSGAHQG 310 PFDHGSGSLS 320 PSKKSPVGKS 330 PPATGSAYGS 340 SQKEESAASG 350 GAAYTKRYLE 360 EQKTENGKDK 370 EQKQTNTDKE 380 KLKEKGGFSD 390 ADVKMKSDPF 400 APKTDTEKPF 410 RGSQSPKRYK 420 LRDDFEKKMA 430 DFHKEEMDEQ 440 DKDKCKGRKE 450 PEFDDEPKFI 460 SKVIAGASKN 470 QEEEKSGKWE 480 SLHAGKEKQR 490 KAEELEEEPF 500 TERSRKEERG 510 GSKRSESGHR 520 GFVPEKNFRV 530 TAYKAVQEKS 540 SSPPPRKTSE 550 SRDKLGNKGD 560 FSSGKSSFSI 570 TREAQVNVRM 580 DSFDEDLARP 590 SGLLAQERKL 600 CRDLVHSNKK 610 EQEFRSIFQH 620 IQSAQSQRSP 630 SELFAQHIVT 640 IVHHVKEHHF 650 GSSGMTLHER 660 FTKYLKRGNE 670 QEAAKTKKSP 680 EIHRRIDISP 690 STFRKHGLTH 700 EELKSPREPG 710 YKAEGKYKDD 720 PVDLRLDIER 730 RKKHKERDLK 740 RGKSRESVDS 750 RDSSHSRERS 760 TEKTEKTHKG 770 SKKQKKHRRA 780 RDRSRSSSSS 790 SQSSHSYKAE 800 EYPEEAEDRE 810 ESTTGFDKSR 820 LGTKDFVGPN 830 ERGGRARGTF 840 QFRARGRGWG 850 RGNYSGNNNN 860 NSNNDFQKRS 870 REEEWDPEYT 880 PKSKKYYLHD 890 DREGEGSDKW 900 MSRGRGRGAF 910 PRGRGRFMFR 920 KSSTSPKWAH 930 DKFSGEEGEI 940 EDDESGTENR 950 EEKDSLQPSA E

Gene Ontology

Classification GO ID Description
Molecular Function GO:0003712 transcription coregulator activity
Cellular Component GO:0035145 exon-exon junction complex
Cellular Component GO:0016592 mediator complex
Cellular Component GO:0016607 nuclear speck
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0003677 DNA binding
Molecular Function GO:0046966 nuclear thyroid hormone receptor binding
Molecular Function GO:0051219 phosphoprotein binding
Molecular Function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
Molecular Function GO:0003713 transcription coactivator activity
Biological Process GO:0007623 circadian rhythm
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0006397 mRNA processing
Biological Process GO:0048255 mRNA stabilization
Biological Process GO:0000956 nuclear-transcribed mRNA catabolic process
Biological Process GO:0042753 positive regulation of circadian rhythm
Biological Process GO:0045893 positive regulation of DNA-templated transcription
Biological Process GO:0048026 positive regulation of mRNA splicing, via spliceosome
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0000381 regulation of alternative mRNA splicing, via spliceosome
Biological Process GO:0008380 RNA splicing

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.