Search Results

Overview

Uniprot ID	Q5MNZ6
Protein Name	WD repeat domain phosphoinositide-interacting protein 3
Gene Name	WDR45B
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
266	AEDPKRNKQSSLASA
278	ASASFLPKYFSSKWS

Function

Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation (PubMed:28561066). Binds phosphatidylinositol 3-phosphate (PtdIns3P), and other phosphoinositides including PtdIns(3,5)P2, forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases and is recruited at phagophore assembly sites where it regulates the elongation of nascent phagophores downstream of WIPI2 (PubMed:28561066, PubMed:30797857). In the cellular response to starvation, may also function together with the TSC1-TSC2 complex and RB1CC1 in the inhibition of the mTORC1 signaling pathway (PubMed:28503735)

Protein Sequence

10 MNLLPCNPHG 20 NGLLYAGFNQ 30 DHGCFACGME 40 NGFRVYNTDP 50 LKEKEKQEFL 60 EGGVGHVEML 70 FRCNYLALVG 80 GGKKPKYPPN 90 KVMIWDDLKK 100 KTVIEIEFST 110 EVKAVKLRRD 120 RIVVVLDSMI 130 KVFTFTHNPH 140 QLHVFETCYN 150 PKGLCVLCPN 160 SNNSLLAFPG 170 THTGHVQLVD 180 LASTEKPPVD 190 IPAHEGVLSC 200 IALNLQGTRI 210 ATASEKGTLI 220 RIFDTSSGHL 230 IQELRRGSQA 240 ANIYCINFNQ 250 DASLICVSSD 260 HGTVHIFAAE 270 DPKRNKQSSL 280 ASASFLPKYF 290 SSKWSFSKFQ 300 VPSGSPCICA 310 FGTEPNAVIA 320 ICADGSYYKF 330 LFNPKGECIR 340 DVYAQFLEMT DDKL

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0005764	lysosome
Cellular Component	GO:0000407	phagophore assembly site
Cellular Component	GO:0034045	phagophore assembly site membrane
Molecular Function	GO:0080025	phosphatidylinositol-3,5-bisphosphate binding
Molecular Function	GO:0032266	phosphatidylinositol-3-phosphate binding
Molecular Function	GO:0030674	protein-macromolecule adaptor activity
Molecular Function	GO:0062078	TSC1-TSC2 complex binding
Biological Process	GO:0000045	autophagosome assembly
Biological Process	GO:0000422	autophagy of mitochondrion
Biological Process	GO:0009267	cellular response to starvation
Biological Process	GO:0061723	glycophagy
Biological Process	GO:0044804	nucleophagy
Biological Process	GO:0000425	pexophagy
Biological Process	GO:0034497	protein localization to phagophore assembly site

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.